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A novel surface plasmon resonance sensor based on a functionalized graphene oxide/molecular-imprinted polymer composite for chiral recognition of <scp>l</scp> -tryptophan
摘要： Herein, a novel surface plasmon resonance (SPR) sensor based on a functionalized graphene oxide (GO)/molecular-imprinted polymer composite was developed for the chiral recognition of L-tryptophan (L-Trp). The composite's recognition element was prepared via a facile and green synthesis approach using polydopamine as both a reducer of GO and a functional monomer as well as a cross-linker for molecular imprinting. The composite was characterized via Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction, and Raman spectroscopy. After attaching the composite onto the gold surface of an SPR chip, the sensor was characterized using contact-angle measurements. The sensor exhibited excellent selectivity and chiral recognition for the template (i.e., L-Trp). Density functional theory computations showed that the difference in hydrogen bonding between the composite element and L-Trp and D-Trp played an important role in chiral recognition.
关键词： molecular-imprinted polymer,graphene oxide,surface plasmon resonance,L-tryptophan,chiral recognition
更新于2025-09-19 17:15:36
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Electroactive Au@Ag NP Assemblies Driven Signal Amplification for Ultrasensitive Chiral Recognition of D-/L-Trp
摘要： A novel ingenious and ultrasensitive chiral electrochemical transducer is proposed for the tryptophan (Trp) isomers detection by using electroactive Au@Ag NPs as electrochemical tags. Moreover, the large binding constant of D-Trp on NPs and strong interaction between D-Trp and Cu2+ cause electroactive Au@Ag NP to assembly on the electrode, generating strong differential pulse voltammetry (DPV) signals from the oxidation of Ag0 to Ag+. In sharp contrast to D-Trp, L-Trp leads to the assembly of Au@Ag NP oligomers on electrode, resulting in a weak DPV signal. The distinct DPV responses enable the developed electrochemical chiral transducer for the sensitive and accurate quantification of D-/L-Trp. The limit of detection (LOD) is 1.21 pM for D-Trp. This established electrochemical chiral sensor also achieves the specific determination of enantiomeric excess. In comparison to other reported approaches, this proposed electrochemical chiral sensor excels by its sensitivity, simplicity and good availability of electroactive Au@Ag NP assemblies. Target-induced colorimetric assays can be converted into electrochemical assays for the dual signal amplification in the field of ultrasensitive enantioselective chiral discrimination.
关键词： Au@Ag NPs,Chiral recognition,Trp enantiomers,Electroactive,Assemblies
更新于2025-09-19 17:15:36
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A highly efficient chiral sensing platform for tryptophan isomers based on a coordination self-assembly
摘要： Construction of convenient and effective method for enantiomer identification is of vital significance for biochemistry and medical science. Herein, we design an effective sensor for chiral recognition of tryptophan (Trp) enantiomers, and self-assembly of Cu2+-modified β-cyclodextrin on poly-L-arginine/multi-walled carbon nanotubes (Cu-β-CD/PLA/MWCNTs) is studied. Meanwhile, Cu2+ acts as a cap to prevent the release of the high energy water and compel Trp enantiomer into the smaller opening of β-cyclodextrin. Recognition of L-Trp is accomplished by the formation of hydrogen bonds between the amino of L-Trp and the the high energy water confined in cavity of Cu-β-CD. Compared with D-Trp, the sensor exhibits favorable chiral recognition toward L-Trp with a separation coefficient of 3.37. And the chiral sensor presents admirable enantiomers determination with excellent sensitivity, providing a good linear correlation in the range of 1×10-6 M~5.5×10-5 M, and the detection limit can reach 3.3×10-7 M (S/N=3). Besides, the proposed sensor has been able to predict the percentage of D-Trp in the racemic mixture, suggesting its potential applications in the enantiomer recognition field.
关键词： Chiral recognition,Cu-β-cyclodextrin,Tryptophan enantiomers,self-assembly
更新于2025-09-09 09:28:46