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Peptide Conjugated CuS Nanocomposites for NIR Triggered Ablation of Pseudomonas aeruginosa Biofilm
摘要： The Gram-negative bacteria Pseudomonas aeruginosa is one famous bacterial strain owing to its ability to effectively form biofilms, which is a front-line mechanism of bacterial tolerance. Herein, the near-infrared-induced nanocomposites were one-step prepared by modifying copper sulfide nanoparticle with peptide to effectively eradicate Pseudomonas aeruginosa biofilm through electrostatic interaction, photodynamic effect and photothermal effect. These nanocomposites could rapidly adhere to the surface of bacteria, and irreversible damage the bacterial membrane under near-infrared laser irradiation. Furthermore, the nanocomposites could selectively eliminate bacteria over mammalian cell without distinct toxicity to NIH 3T3 cells. The nanocomposites will exert a far-reaching impact on the future design of biocompatible near-infrared-induced antibacterial agents, exhibiting its potential applications in Gram-negative bacteria and biofilm infections.
关键词： copper sulfide nanoparticles,near-infrared laser,Gram-negative bacterial biofilm,photothermal and photodynamic effect
更新于2025-11-14 17:03:37
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Integrin α <sub/>γ</sub> β <sub/>3</sub> -targeted [ <sup>64</sup> Cu]CuS Nanoparticles for PET/CT Imaging and Photothermal Ablation Therapy
摘要： Copper sulfide (CuS) nanoparticles have been considered one of the most clinical relevant nanosystems because of their straightforward chemistry, small particle size, low toxicity, and intrinsic theranostic characteristics. In our previous studies, radioactive [64Cu]CuS nanoparticles were successfully developed to be used as efficient radiotracers for positron emission tomography and for photothermal ablation therapy of cancer cells using near-infrared laser irradiation. However, the major challenge of CuS nanoparticles as a theranostic platform is the lack of a means for effective targeted delivery to the tumor site. To overcome this challenge, we designed and synthesized angiogenesis-targeting [64Cu]CuS nanoparticles, which are coupled with cyclic RGDfK peptide [c(RGDfK)] through polyethylene glycol (PEG) linkers using click chemistry. In assessing their tumor-targeting efficacy, we found that the tumor uptakes of [64Cu]CuS-PEG-c(RGDfK) nanoparticles at 24 h after intravenous injection were significantly greater (8.6%±1.4% injected dose/gram of tissue) than those of nontargeted [64Cu]CuS-PEG nanoparticles (4.3%±1.2% injected dose/gram of tissue, p < 0.05). Irradiation of tumors in mice administered [64Cu]CuS-PEG-c(RGDfK) nanoparticles induced 98.7% necrotic areas. In contrast, irradiation of tumors in mice administered non-targeted CuS-PEG nanoparticles induced 59% necrotic areas (p < 0.05). The angiogenesis-targeting [64Cu]CuS nanoparticles may serve as a promising platform for image-guided ablation therapy with high efficacy and minimal side effects in future clinical translation of this novel class of multifunctional nanomaterials.
关键词： PET/CT imaging,RGD peptide,Copper sulfide nanoparticles,photothermal ablation therapy,integrin αvβ3,theranostics
更新于2025-09-23 15:21:21