- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
<p>A multifunctional-targeted nanoagent for dual-mode image-guided therapeutic effects on ovarian cancer cells</p>
摘要： Nanomedicine has emerged as a novel therapeutic modality for cancer treatment and diagnosis. Lipid–polymer hybrid nanoparticles (LPHNPs) are core–shell nanoparticle (NP) structures comprising polymer cores and lipid shells, which exhibit complementary characteristics of both polymeric NPs and liposomes. However, it is difficult to wrap perfluoropentane (PFP) into core–shell NPs in the existing preparation process, which limits its application in the integration of diagnosis and treatment. Methods: The folate-targeted LPHNPs-loaded indocyanine green/PFP-carrying oxygen (TOI_HNPs) using a combination of two-step method and solution evaporation technique for the first time. The essential properties and dual-mode imaging characteristics of developed NPs were determined. The cellular uptake of TOI_HNPs was detected by confocal microscopy and flow cytometry. The SKOV3 cell viability and apoptosis rate were evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay and flow cytometry. The ROS was demonstrated by fluorescence microplate reader and the expression of hypoxia-inducible factor 1-alpha (HIF-1α) and IL-6 was detected by Western blot. Results: TOI_HNPs showed spherical morphology with particle size about (166.83±5.54) nm and zeta potential at -(30.57±1.36) mV. It exhibited better stability than lipid NPs and higher encapsulation efficiency as well as active targeting ability than poly (lactic-co-glycolic acid) (PLGA) NPs. In addition, the novel NPs could also act as the contrast agents for ultrasound and photoacoustic imaging, providing precision guidance and monitoring. Furthermore, TOI_HNPs-mediated photo–sonodynamic therapy (PSDT) caused more serious cell damage and more obvious cell apoptosis, compared with other groups. The PSDT mediated by TOI_HNPs induced generation of intracellular ROS and downregulated the expression of HIF-1α and IL-6 in SKOV3 cells. Conclusion: This kind of multifunctional-targeted nanoagent may provide an ideal strategy for combination of high therapeutic efficacy and dual-mode imaging guidance.
关键词： core-shell nanoparticle,ultrasound,photo-sonodynamic therapy,phase transformation,photoacoustic imaging,laser
更新于2025-11-14 17:03:37
-
Preparation and application of polystyrene-grafted alumina core-shell nanoparticles for dielectric surface passivation in solution-processed polymer thin film transistors
摘要： Polystyrene-grafted alumina nanoparticles were synthesized by silane coupling between dimethylchlorosilane-terminated polystyrene (PS) and gamma-type alumina nanoparticles and characterized. The surface grafting density of the polystyrene chains on the nanoparticles was estimated to be 0.13 molecules per square nanometer. The Al2O3-PS nanoparticles are solution processable in organic solvents, including ethyl acetate, butyl acetate, and toluene, which enabled preparation of blends with polystyrene or poly(methyl methacrylate). The dielectric constant of the Al2O3-PS nanoparticle/polymer blend films is composition tunable from 2.59 to 7.79. The alumina-PS nanoparticles and their blends with polymers were found to form efficient surface passivation films on the oxide dielectric layer in organic field-effect transistors (OFETs).
关键词： core-shell nanoparticle,alumina-polymer gate insulator,organic field-effect transistor,surface modification
更新于2025-09-09 09:28:46