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Bacteria-Targeted Supramolecular Photosensitizer Delivery Vehicles for Photodynamic Ablation Against Biofilms
摘要： Photodynamic therapy (PDT) is believed to be a potent method for biofilm treatments. However, undesired damage to normal cells may be caused due to the nonselective nature of PDT. Therefore, targeted PDT is preferred on one hand to enhance antimicrobial effects and on the other hand to reduce cytotoxicity to normal cells. For this purpose, novel bacteria-targeted photosensitizer delivery micelles are fabricated, taking advantage of α-cyclodextrin (α-CD)/polyethylene glycol (PEG) supramolecular assembly. Hydrophilic antimicrobial peptide (AMP) Magainin I is covalently bound with PEG, working as a bacterial targeting group as well as the stabilizing shell of the supramolecular micelles. Photosensitizer Chlorin e6 (Ce6) is grafted onto α-CD. The micelles exhibit excellent bacterial targeting effects. Compared to α-CD-Ce6, the supramolecular micelles possess enhanced biofilm killing ability against Gram (?) Pseudomonas aeruginosa biofilms and Gram (+) methicillin-resistant Staphylococcus aureus (MRSA) biofilms while reducing cytotoxicity to NIH/3T3 model cells.
关键词： antimicrobial peptides,biofilms,targets,cytocompatibility,photodynamic therapy
更新于2025-09-23 15:21:01
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Study on Fe-xGO Composites Prepared by Selective Laser Melting: Microstructure, Hardness, Biodegradation and Cytocompatibility
摘要： The problem of the degradation rate being too slow is a key technical bottleneck to clinical applications for pure iron (Fe), a promising candidate biodegradable metal. This work used powders of pure Fe and graphene oxide (GO) to prepare Fe-xGO composites (x = 0.4 wt.%, 0.8 wt.%, 1.2 wt.%, and 1.6 wt.%) via selective laser melting (SLM), aiming to obtain a higher degradation rate. The microstructure, hardness, biodegradation and cytocompatibility were investigated. The degradation rate of the SLMed Fe-xGO composites was faster than that of SLMed Fe, due to incorporating GO into Fe. The GO content had a significant effect on the microstructure, hardness and degradation rate. The SLMed Fe-0.8 GO composite presented the finest, relatively uniform grains, had the maximum degradation rate, density and hardness, and had good cytocompatibility. The mechanisms were also clarified.
关键词： biodegradation,Fe-xGO composites,hardness,cytocompatibility,selective laser melting,microstructure
更新于2025-09-16 10:30:52
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Bioinspired crystallization, sensitized luminescence and cytocompatibility of citrate-functionalized Ca-substituted europium phosphate monohydrate nanophosphors
摘要： Biocompatible nanosystems exhibiting long-lifetime (~millisecond) luminescence features are particularly relevant in the field of bioimaging. In this study, citrate-functionalized calcium-doped europium phosphates nanophosphors of the rhabdophane type were prepared at different synthesis times by a bioinspired crystallization route, consisting in thermal decomplexing of Ca2+/Eu3+ /citrate/phosphate/carbonate solutions. The general formula of this material is Ca?Eu1-?(PO4)1-?(HPO4)?·nH2O, with ??ranging from 0 to 0.58 and n~1. A thorough characterization of the nanoparticles has been carried out by XRD (including data processing with Topas 6.0), HR-TEM, TEM, FTIR, TG/DTA, ICP, dynamic light scattering (DLS), electrophoretic mobility, and fluorescence spectroscopy. Based on these results a crystallization mechanism involving the filling of cationic sites with Ca2+ ions associated to a concomitant adjustment of the PO4/HPO4 ratio was proposed. Upon calcium doping, the aspect ratio of the nanoparticles as well as of the crystalline domains decreased and the relative luminescence intensity (R.L.I.) could be modulated. Neither the pH nor the ionic strength, nor the temperature (from 25 to 37 oC) affected significantly the R.L.I. of particles after resuspension in water, leading to rather steady luminescence features usable in a large domain of conditions. This new class of luminescent compounds has been proved to be fully cytocompatible relative to GTL-16 human carcinoma cells and showed an improved cytocompatibility as the Ca2+ content increased when contacted with the more sensitive m17.ASC murine mesenchymal stem cells. These biocompatible nanoparticles thus appear as promising new tailorable tools for biomedical applications as luminescent nanoprobes.
关键词： Nanophosphors,Citrate-functionalized,Luminescence,Bioimaging,Cytocompatibility,Calcium-doped europium phosphates
更新于2025-09-10 09:29:36