Non-Invasive Plasmonic Based Real Time Characterization of Cardiac Drugs on Cardiomyocytes Functional Behavior

摘要： In fabrication of cardiac tissue, an important factor is continuous measurement of its contraction features. A module that allows for a dynamic system capable of non-invasive and label-free monitoring of contraction profile under administering chemicals and drugs, is highly valuable for understanding accurate tissue mechanobiology. In this research, we have successfully demonstrated the use of Surface Plasmon Resonance (SPR) technology for the first time to characterize the contractility of cardiac cells in response to Blebbistatin and ATP as drug exposure in real-time. An optimal flow rate of 10 μlit/min was selected for continuous flow of warm media and 10 μM drug administration effect was detected with high spatiotemporal sensitivity on contracting cardiomyocytes. Our drug screening has identified the source of SPR periodic signal to be directly cell contraction rather than action potentials or calcium signaling. Per our results, SPR has high potential in applications in least interference real-time and label-free tissue characterizations and cellular properties analysis from a functional and structural point of view.