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Green Fluorescent Protein-Based Glucose Indicators Report Glucose Dynamics in Living Cells
摘要： Glucose is the most important energy source for living animals. Here, we developed a series of single fluorescent protein (FP)-based glucose indicators, named as "Green Glifons", to understand the hierarchal and mutual relationships between molecules involved in energy metabolism. Three indicators showed a different EC50 for glucose (50 μM, 600 μM and 4,000 μM), producing a ~7-fold change in fluorescence intensity in response to glucose. The indicators could visualize glucose dynamics in the cytoplasm, plasma membrane, nucleus and mitochondria of living HeLa cells and in vivo, in the pharyngeal muscle of C. elegans and could measure murine blood glucose levels. Finally, the indicators were applicable to dual-color imaging, revealing the dynamic interplay between glucose and Ca2+ in mouse pancreatic MIN6 m9 β cells. We propose that these indicators will facilitate and contribute to in vivo and multi-color imaging of energy metabolism.
关键词： biosensors,artificial sweeteners,dual-color imaging,C. elegans,live cell imaging,glucose,blood glucose level,fluorescent protein
更新于2025-11-21 11:24:58
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An Electrically Modulated Singlea??Color/Duala??Color Imaging Photodetector
摘要： An electrically modulated single-/dual-color imaging photodetector with fast response speed is developed based on a small molecule (COi8DFIC)/perovskite (CH3NH3PbBr3) hybrid film. Owing to the type-I heterojunction, the device can facilely transform dual-color images to single-color images by applying a small bias voltage. The photodetector exhibits two distinct cut-off wavelengths at ≈544 nm (visible region) and ≈920 nm (near-infrared region), respectively, without any power supply. Its two peak responsivities are 0.16 A W?1 at ≈525 nm and 0.041 A W?1 at ≈860 nm with a fast response speed (≈102 ns). Under 0.6 V bias, the photodetector can operate in a single-color mode with a peak responsivity of 0.09 A W?1 at ≈475 nm, showing a fast response speed (≈102 ns). A physical model based on band energy theory is developed to illustrate the origin of the tunable single-/dual-color photodetection. This work will stimulate new approaches for developing solution-processed multifunctional photodetectors for imaging photodetection in complex circumstances.
关键词： organic/perovskite photodetectors,response speed,dual-color imaging
更新于2025-09-23 15:21:01
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Dual-color near-field imaging by means of thin-film plasmonic waveguide with precise beam control of multiple wavelengths
摘要： We describe an improved optical beam control technique combined with a waveguide probe for imaging optical near-field using multiple wavelengths. The beam control technique varies the incident angle of pseudocollimated beams whose spot diameter is ～10 μm such that it is equal to an arbitrary angle with ～0.03○ precision and the beam position to ～0.1 μm precision. This helps resonantly excite surface plasmons using visible 660 nm and near-infrared 850 nm on an ～10 μm width three-dimensional microwaveguide. We demonstrate the operation of this system by imaging the optical near-field and capturing the interference of surface plasmons in a gold microstructure. This system achieved dual-color imaging of the optical near-field of different modulation frequencies with submicrometer lateral spatial resolution.
关键词： surface plasmons,optical beam control,multiple wavelengths,dual-color imaging,submicrometer lateral spatial resolution,waveguide probe
更新于2025-09-12 10:27:22