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Detection of Various Biomarkers and Enzymes via Nanocluster-Based Fluorescence Turn-On Sensing Platform
摘要： The profiling of disease-related biomarkers is an essential procedure for the accurate diagnosis and intervention of metabolic disorders. Therefore, the development of ultrasensitive and highly selective fluorogenic biosensors for diverse biomarkers is extremely desirable. There is still a considerable challenge to prepare nanocluster-based fluorescence turn-on probes capable of recognizing multiple biomolecules. We herein provide a novel nanocluster-based chemical information processing system (CIPS) for the programmable detecting various metabolites and enzymes. This CIPS consists of the biocatalytic reactions between substrates and their respective oxidases to generate H2O2, which was then employed to synthesize bright fluorescent silver nanoclusters (Ag NCs). Utilizing this system, we are able to accurately probe a series of substrates/ corresponding oxidases with high sensitivity and specificity, including glucose/ glucose oxidase, uric acid/ uric acid oxidase, sarcosine/ sarcosine oxidase, choline/ choline oxidase, xanthine/ xanthine oxidase, and lactic acid/ lactic acid oxidase. Furthermore, this metabolite profiling CIPS can integrate with binary logic operations, which create an intelligently tool for the high throughput screening of various diseases in vitro (e.g., diabetes, gout, prostate cancer, cardiovascular disease, and lactic acidosis).
关键词： nanocluster-based fluorescence turn-on sensing platform,silver nanoclusters,chemical information processing system,biomarkers,enzymes
更新于2025-09-09 09:28:46
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A comprehensive review on thin film-based nano-biosensor for uric acid determination: arthritis diagnosis
摘要： This article aims to provide a survey on the development of thin film-based nano-biosensor over the decade, which includes sensing principles, electrode types and various thin film matrices. The theoretical and experimental results suggest that the thin film-based sensor are suitable for highly sensitive detection of low concentration bio molecules and can be used for early diagnosis and screening of arthritis, kidney diseases and other cardiovascular diseases. This review concludes that enzyme immobilisation and surface functionalisation methods were used to increase sensitivity of the biosensor and new combination of polymer nano-composites with metal oxide thin films results in increased efficiency due to its high biocompatibility.
关键词： nanomaterials,enzymes,thin films,uric acid,immobilisation,biocompatibility
更新于2025-09-09 09:28:46
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UV irradiation induces resistance against fruit rot disease and improves the quality of harvested mangosteen
摘要： Fruit rot disease and short shelf life are the limiting factors of mangosteen fruit. This study focused on investigating the effect of ultraviolet (UV) irradiation in controlling fruit rot disease and on quality improvement in harvested mangosteen. Mangosteen fruit artificially inoculated with L. theobromae were treated with UV-B or UV-C irradiation at dosages of 0 (control), 6, 13, 26, and 40 kJ m?2 and stored at 25 °C for 7 d. The results indicate that UV-C treatment exhibited higher fungicidal potential in preventing fruit rot disease than UV-B treatment. UV-C treatment at a dose of 13 kJ m?2 was the most effective in reducing disease incidence and lesion diameter, followed by UV-C at a dose of 26 kJ m?2 and a lower dosage (6 kJ m?2) showed no significant difference from the untreated fruit. Therefore, UV-C irradiation at doses of 13 kJ m?2 was selected to investigate the induction of disease resistance and improvement of mangosteen quality. Naturally infected mangosteen fruit were exposed to UV-C irradiation at a dose of 13 kJ m?2 and untreated fruit served as the control. All fruit samples were kept at 13 °C for 21 d. The results showed that UV-C treatment reduced fruit rot disease and induced the activity of key plant defense-related enzymes, including phenylalanine ammonia lyase (PAL), peroxidase (POD), chitinase and β-1,3-glucanase, besides increasing the content of total phenolics and hydrogen peroxide (H2O2). In addition, the fruit treated with UV-C could delay weight loss, respiration rate, color changes of peel and calyx and maintain total chlorophyll in the calyx of mangosteen. The results suggest that UV-C application is an alternative approach to suppress fruit rot disease and improve the postharvest quality of mangosteen.
关键词： Mangosteen fruit,Fruit rot disease,Defense related enzymes,UV irradiation
更新于2025-09-04 15:30:14