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- 实验方案
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Fluorescence-Guided Cancer Diagnosis and Surgery by a Zero Cross-Talk Ratiometric Near-Infrared γ-Glutamytranspeptidase Fluorescent Probe
摘要： The ability to early detect cancer in an accurate and rapid fashion is of critical importance for cancer diagnosis and accurate resection in surgery. γ-Glutamyltranspeptidase (GGT) is overexpressed in several human cancers while keeps low expression in normal microenvironment, and thus recognized as an important cancer biomarker. To date, rational design of a zero cross-talk ratiometric near-infrared (NIR) GGT fluorescent probe for efficiently cancer diagnosis in various biological samples is still a big challenge. In this work, a zero cross-talk ratiometric NIR GGT fluorescent probe named Cy-GSH is developed. Cy-GSH shows highly sensitive to GGT, which is desired for cancer early diagnosis. Upon additional GGT, a large emission shift from 805 nm to 640 nm is observed, which is suitable for visualizing deeply located cancer in vivo. In addition, successful monitoring GGT activity in bloods, cells, tissues and in vivo, makes Cy-GSH possess great potentials for the clinical cancer early diagnosis. Furthermore, accurate visualizing tumors and metastases in mouse models illuminates that the probe may be a convenient tool for fluorescence-guided cancer surgery. To our knowledge, this is the first report to describe the strategy of a zero cross-talk ratiometric NIR GGT fluorescent probe for cancer early diagnosis and fluorescence-guided surgery.
关键词： fluorescent probe,γ-Glutamyltranspeptidase,GGT,cancer diagnosis,fluorescence-guided surgery
更新于2025-09-04 15:30:14
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Nitrogen-Doped Durian Shell Derived Carbon Dots for Inner Filter Effect Mediated Sensing of Tetracycline and Fluorescent Ink
摘要： Photoluminescent carbon dots have gained increasing attention in recent years due to their unique optical properties. Herein, a facile one-pot hydrothermal process is used to develop nitrogen-doped carbon dots (NCDs) with durian shell waste as the precursor and Tris base as the doping agent. The synthesized NCDs showed a quantum yield of 12.93% with a blue fluorescence under UV-light irradiation and maximum emission at 414 nm at an excitation wavelength of 340 nm. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy showed the presence of nitrogen and oxygen functional groups on the NCD surface. The particles were quasi-spherical with an average particle diameter of 6.5 nm. The synthesized NCDs were resistant to photobleaching and stable under a wide range of pH but were negatively affected by increasing temperature. NCDs showed high selectivity to Tetracycline as the fluorescence of NCDs was quenched significantly by Tetracycline as a result of the inner filter effect. Based on sensitivity experiments, a linear relationship (R2 = 0.989) was developed over a concentration range of 0–30 μM with a detection limit of 75 nM (S/N = 3). The linear model was validated with two water samples (lake water and tap water) with relative recoveries of 98.6–108.5% and an RSD of <3.5%.
关键词： Fluorescent ink,Tetracycline detection,Durian shell waste,Nitrogen doping,Carbon dots
更新于2025-09-04 15:30:14
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A ratiometric fluorescent probe for hydrazine detection with large fluorescence change ratio and its application for fluorescence imaging in living cells
摘要： Hydrazine and its derivatives are one of the most important raw chemical materials that have extensive utilization in many areas. It is also widely applied as pharmaceutical derivatives, such as the antipsychotic drug, anti-infective drug, and antitumor drug. However, hydrazine is a highly toxic reagent which can bring severe damage to human and animals. Herein, we have reported a new benzothiazole substituted naphthalene based probe for hydrazine with huge fluorescence change ratio. The fluorescence change ratio at F390/F509 is up to 200-fold in the presence of hydrazine in 60 min. The probe exhibited negligible cytotoxicity toward HeLa cells and was applied for hydrazine detection successfully in HeLa cells.
关键词： Fluorescent probe,ratiometric,cell imaging,hydrazine detection
更新于2025-09-04 15:30:14
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Polymeric Micelles Encapsulating a Small Molecule SO <sub/>2</sub> Fluorescent Probe Exhibiting Novel Analytical Performance and Enhanced Cellular Imaging Ability
摘要： Because of the limited knowledge on the relationship between molecular structure and analytical performance, developing a small molecule fluorescent probe with desirable response properties is usually a laborious work. On the other hand, the application of small molecule fluorescent probe in biological samples is always limited due to the unwanted interaction between dyes and biomacromolecules. Polymer micelles, thanks to its unique core?shell structure, may have the potential to improve these situations. However, utilization of polymer micelles to improve these situations is rarely explored. Herein, we engineered the first micellar SO2 nanoprobe Nano-Cz by self-assembly of a carbazole-based SO2 small molecule probe and an amphiphilic copolymer (DSPE-mPEG2000). The optical and cell imaging experiments revealed that Nano-Cz can work in 100% aqueous environment and act as an effective mitochondrial-targeting ratio SO2 nanoprobe. Compared with the single small molecule probe, Nano-Cz showed extraordinary large dynamic response range (0?0.7 mM vs 0?50 μM), eliminated signal interference from DNA and superior cellular imaging performance. These results clearly show the ability of polymer micelles in modulating sensors’ analytical performance and reducing the signal interference from the unwanted interaction between small molecule probe and biomacromolecule, indicating that polymer micelles encapsulating single small molecule probe can provide us an alternative strategy to explore sensors with various performance and promote the biological application of fluorescent sensors. In addition, we hope that more and more polymer micelles would be used to construct biosensors in the future.
关键词： sensing performance,biological application,sulfur dioxide,polymer micelles,fluorescent sensor
更新于2025-09-04 15:30:14
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Indian Gooseberry-Derived Tunable Fluorescent Carbon Dots as a Promise for In Vitro/In Vivo Multicolor Bioimaging and Fluorescent Ink
摘要： We report the synthesis of eco-friendly fluorescent nitrogen-doped carbon dots (NCDs) using the renewable resource of Phyllanthus emblica juice as a precursor by the hydrothermal process at 200 °C for 12 h. The synthesized NCDs emitted bright fluorescence without any pretreatment of the sample under the excitation of UV light and exhibited excitation-dependent fluorescence emission. The NCDs have nitrogen-containing and oxygen-containing functional groups such as amino, hydroxyl, and carboxyl on the surface of the carbon structure. Furthermore, the NCDs exhibited excellent water dispersibility with prolonging stability and good biocompatibility. On the basis of the good optical properties, the NCDs have potentially been used as a promising staining agent on HCT-116 human colon cancer cells and Caenorhabditis elegans (nematodes) for multicolor cellular imaging. In the cell cytoplasm, the NCDs showed rapid uptake and high cytocompatibility on cellular morphology with bright fluorescence emission. Furthermore, the NCDs were used as fluorescent ink for writing and drawing with anticoagulation. In addition, the NCDs were significantly utilized as a fluorescent ink for thumb impression, which glows instantly under the illumination of UV light and does not require a secondary treatment. Hence, the synthesized NCDs can be used as ideal multicolor fluorescent probes for bioimaging applications and as fluorescent ink instead of traditional fluorescent ink.
关键词： nitrogen-doped,hydrothermal synthesis,carbon dots,fluorescent ink,bioimaging
更新于2025-09-04 15:30:14
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On/off rhodamine-BODIPY based fluorimetric/colorimetric sensor for detection of mercury(II) in half-aqueous medium
摘要： A turn on/off rhodamine-BODIPY (RB) fluorescent sensor has been designed for selective and sensitive detection of Hg(II) cation in half-aqueous solution. For this purpose, a Bodipy derivative having dual-cores is bilaterally bound to a rhodamine containing dual-amine moieties. The newly prepared sensor reacts with mercury (II) salt to generate a product with different optical parameters. These are changes confirmed by both absorption, emission and excitation measurements. “A blue shift is observed as the ‘Turn-off’ change the pink color to transparent upon the addition of Hg (II) ions which is assigned to suppression of fluorescence resonance energy transfer (FRET) upon mercury (II) ion affinity. Selectivity towards Hg(II) ion is found to depend upon cavity size created of the amino moieties of rhodamine and the carbonyls of Bodipy to the metal ion. Sensor RB is selective and sensitive to Hg(II) with a detection limit (1.94 (?0.2) 10-8 M). The three-way fluorescent probe can be selectively applied for the detection of mercury (II) ion in real water samples.
关键词： Bodipy,emission,mercury,absorption,fluorescent,Rhodamine
更新于2025-09-04 15:30:14
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Co-localization coefficients of a target-switchable fluorescent probe can serve as an indicator of mitochondrial membrane potential
摘要： The mitochondrial membrane potential (MMP) definitely reflects mitochondrial function. Thus, it is very essential to found a physical parameter as MMP indicator. At present, available parameters are either fluorescent intensity of monochromatic probes such as rhodamine 123 or a ratio of fluorescent intensity at different wavelengths of dual-color dyes such as JC-1, but the inconvenience in practice as well as serious effect of loading concentrations on experimental results limited their application. To address this concern, herein, we found a reliable and easily obtainable co-localization coefficient (CLC) of a fluorescent probe as new MMP indicator and developed a target switchable fluorescent probe (Mito-Lyso) to attain the aim. Due to intrinsic nature, Mito-Lyso exclusively stains mitochondria with normal MMP and a subsequent decreasing of MMP results in release of some Mito-Lyso. Importantly, the released Mito-Lyso can reversibly transfer between mitochondria and lysosomes. Thus, CLCs of Mito-Lyso and a commercial lysosomal probe (NIR-Lyso) can be MMP-dependent. CLCs gradually increased from 0.20 to 0.8 with the decreasing of MMP and then returned to 0.3 with the recovering of MMP, which better proves that the CLC is a valuable MMP indicator. Furthermore, both the design principle and action mechanism of Mito-Lyso has been explained in detail for the development of this type of probes.
关键词： Mito-Lyso,fluorescent probe,co-localization coefficient,mitochondrial membrane potential,target-switchable
更新于2025-09-04 15:30:14
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On the Fluorescent, Steric and Electronic Factors Affecting the Detection of Metallic Ions Using an Imidazolyl-Phenolic Derived Fluorescent Probe
摘要： The imidazolyl-phenolic probe used at the present study has its photophysic properties regulated by a tautomeric equilibrium. After the absorption of a photon, an excited state intramolecular proton transfer process generates a ketonic species, responsible for the 440 nm emission (in CH3CN/H2O, 95:5, v/v). Addition of Cu2+, Al3+, Cr3+ and Fe3+ suppresses emission through a combination of dynamic and static-like quenching, as indicated by Stern-Volmer plots, with a higher sensitivity for Cu2+ (KSV = 1.90 × 105 and 2.40 × 104 L mol–1, respectively, for Cu2+ and Fe3+). The trivalent ions led to the formation of a locked-enol tautomer that emits at shorter wavelengths; this coordinated compound is also quenched at metallic ions concentrations above 20 μmol L–1, due to a collisional process. When compared to another imidazolyl-phenolic probe, experimental and simulated data revealed that fluorescent, steric and electronic effects regulate their sensitivity towards the ions.
关键词： iron(III),copper(II),fluorescent sensor,ESIPT,tautomerization
更新于2025-09-04 15:30:14