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oe1(光电查) - 科学论文

2 条数据
?? 中文（中国） ▾

  • Near-infrared light controlled fluorogenic labeling of glycoengineered sialic acids <i>in vivo</i> with upconverting photoclick nanoprobe

    摘要： Near-infrared light controlled fluorogenic labeling of glycoengineered sialic acids in vivo with upconverting photoclick nanoprobe. Sialic acid serves as an important determinant for profiling cell activities in diverse biological and pathological processes. The precise control of sialic acid labeling to visualize its biological pathways under endogenous conditions is significant but still challenging due to the lack of reliable methods. Herein, we developed an effective strategy to spatiotemporally label the sialic acids with a near-infrared (NIR) light activated upconverting nanoprobe (Tz-UCNP). With this photoclickable nanoprobe and a stable N-alkene-D-mannosamine (Ac4ManNIPFA), metabolically synthesized alkene sialic acids on the cell surface were labeled and imaged in real time through fluorogenic cycloaddition. More importantly, we achieved spatially selective visualization of sialic acids in specific tumor tissues of the mice under NIR light activation in a spatially controlled manner. This in situ controllable labeling strategy thus enables the metabolic labeling of specific sialic acids in complex biological systems.

    关键词： fluorogenic labeling,sialic acids,Near-infrared light,upconverting nanoprobe,photoclick chemistry

    更新于2025-09-23 15:21:01

  • Fluorogenic “photoclick” labelling of DNA using a Cy3 dye

    摘要： Two 2’-deoxyuridines as new building blocks for automated DNA synthesis carry a small aryltetrazole as “photoclickable” group at their 5-positions. The postsynthetic “photoclick” labeling of such presynthesized DNA using a maleimide-modified Cy3 dye shows an up to 17-fold fluorogenicity due to an energy transfer between the pyrazoline moiety and the Cy3 fluorophore in the DNA products. This concept is also applicable to other maleimide-modified dyes.

    关键词： Fluorogenic labeling,DNA,tetrazole,photoclick reaction,energy transfer,Cy3 dye

    更新于2025-09-19 17:15:36