- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Rapid detection of sulfamethoxazole in plasma and food samples with in-syringe membrane SPE coupled with solid-phase fluorescence spectrometry
摘要： In this work, in-syringe membrane solid-phase extraction (MSPE) device was fabricated for the on-site sampling of sulfamethoxazole (SMX) in food samples followed by solid-phase fluorescence spectra analysis. The samples and fluorescamine (FA) were added to a syringe for derivation. Then, the derivative of SMX was extracted by a membrane in the syringe SPE device. Subsequently, the derivative on the membrane was measured immediately without additional elution procedure. The method was successfully applied in plasma, milk, and egg samples for the trace SMX detection, with the recovery of 98 % to 102 %, RSDs from 1 % to 6 %. Compared with liquid chromatography, direct detection of the concentrated analyte significantly improved the sensitivity. Moreover, fluorescamine made it unnecessary to separate SMX from the interference. Consequently, it was a time-saving, low-cost, and easy-operation method, which demonstrated the potential of in-syringe SPE as a promising candidate for on-site analysis.
关键词： Sulfamethoxazole,Food samples,In-syringe solid-phase extraction,Solid-phase fluorescence,Fluorescamine
更新于2025-09-23 15:19:57
-
An ultra-selective fluorescence method with enhanced sensitivity for the determination of manganese (VII) in food stuffs using carbon quantum dots as nanoprobe
摘要： A novel fluorescence method was for the first time established for the efficient determination of permanganate (Mn(VII)) based on sulfur, chlorine and nitrogen co-doped carbon quantum dots (S,Cl,N-CQDs). The S,Cl,N-CQDs was prepared via a fast and simple acid-base neutralization method. The fluorescence of S,Cl,N-CQDs could be dramatically quenched by Mn(VII) ascribed to a combination of inner filter effect (IFE) and dynamic quenching. Under the optimal working conditions, the S,Cl,N-CQDs-based nanoprobe exhibited high selectivity and sensitivity towards Mn(VII) sensing. A good linear Stern-Volmer equation was obtained for Mn(VII) in a wide concentration range of 0.05–110 μM with a detection limit as low as 12.8 nM. The proposed fluorescence method also exhibits additional advantages of great simplicity, fast in analysis and low cost. The as-fabricated fluorescent nanoprobe was successfully applied for the determination of Mn(VII) monitoring in complex food matrix.
关键词： Cl,fluorescence quenching,Mn(VII),S,food samples,N-CQDs
更新于2025-09-19 17:13:59
-
Development of Rapid, Sensitive, and Effective Plasmonic Nanosensor for the Detection of Vitamins in Infact Formula and Milk Samples
摘要： The aim of the present study is to develop a surface plasmon resonance sensor for the detection of vitamin B2, vitamin B9, and vitamin B12 in food samples by using the molecular imprinting technique. The vitamin B2, vitamin B9, and vitamin B12 imprinted and the non-imprinted surface plasmon resonance sensor chip surfaces were characterized by using contact angle measurements, atomic force microscopy, ellipsometry, and Fourier transform infrared-attenuated total reflectance. The real-time detection of vitamin B2, vitamin B9, and vitamin B12 was analyzed by using aqueous solutions in the concentration range of 0.01 ng/mL ? 10 ng/mL for vitamin B2, 0.1 ng/mL ? 8.0 ng/mL for vitamin B9, and 0.01 ng/mL ? 1.5 ng/mL for vitamin B12. The limit of detection values was calculated as 1.6×10?4 ng/mL for vitamin B2, 13.5×10?4 ng/mL for vitamin B9, and 2.5×10?4 ng/mL for vitamin B12, respectively. Selectivity experiments were performed by using vitamin B1 and vitamin B6. The reproducibility of surface plasmon resonance sensors was investigated both on the same day and on different days for four times. Validation studies of the surface plasmon resonance sensors were performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS).
关键词： surface plasmon resonance,Vitamin,food samples,molecular imprinting
更新于2025-09-19 17:13:59