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Gold nanoparticles of different shape for bicolor lateral flow test
摘要： Spherical gold nanoparticles are the most commonly used marker in lateral flow assays. However, the widespread practice of using identical coloration for the test and control zones of test strips can lead to erroneous interpretations of the assay’s results. We propose an immunochromatographic test strip with lines of different colors. For this purpose, gold nanoparticles of different shapes were used, namely blue nanoflowers in the test zone and red gold nanospheres in the control zone. A detailed synthesis procedure for nanoparticles and their conjugates is considered and design parameters for optimal results are described. For the first time, nanoparticles of different shapes have been combined in the test strip with indirect labeling of specific antibodies (via their interaction with labeled secondary antibodies). Using the T-2 toxin (T2T) as an example, an instrumental detection limit of 30 pg/mL and a working range 0.06-0.9 ng/mL were achieved in an analysis of water-organic corn extracts.
关键词： gold nanoparticles,Immunochromatography,test strip,mycotoxin,indirect labeling,gold nanoflowers
更新于2025-09-23 15:23:52
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A Strategy for In-Situ Imaging of Cellular Alkaline Phosphatase Activity Using Gold Nanoflower Probe and Localized Surface Plasmon Resonance Technique
摘要： In this work, a simple and ultrasensitive localized surface plasmon resonance (LSPR) method that use Au nanoflowers (AuNFs) as probe was designed for in-situ monitoring of alkaline phosphatase (ALP) activity. The AuNFs was fabricated by hydrogen tetrechloroaurate-induced oxidative disruption of polydopamine-coated Au nanoparticles (AuNPs) and subsequently growth of Au nanopetals on AuNPs. The as-prepared AuNFs showed a much higher LSPR capability and stronger scattering color change than AuNPs. The strategy for in-situ cellular ALP activity detection relied on the deposition of Ag on AuNFs surface, which changed the morphology of AuNFs and led to a tremendous LSPR response and scattering color change. The deposition of Ag shell on AuNFs was related to ALP activity, where ALP catalyzed the hydrolysis of L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate to form L-ascorbic acid (AA), then AA reduced Ag+ to Ag and deposited onto AuNFs. With this concept, the ALP activity could be monitored with a detection limit of 0.03 μU L-1. Meanwhile, the ALP activity of single HepG2 cells and HEK 293 cells was tracked with proposed approach, which indicated the trace expression level of ALP in HEK 293T cell and overexpressed level of ALP in HepG2 cells. After treated with drugs, the cellular ALP activity of HepG2 cells was decreased with the treating time and dose increasing. Therefore, the proposed strategy could be used for tracking the cellular ALP activity, which paved a new avenue for cell studies, and hold great potential for discovering novel ALP-based drugs applications.
关键词： cellular activity,alkaline phosphatase,localized surface plasmon resonance,in-situ imaging,gold nanoflowers
更新于2025-09-23 15:21:21
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Gold nanoflowers grown in a porous Si/SiO2 matrix: the fabrication process and plasmonic properties
摘要： Highly branched metal nanostructures are of great interest for creating highly sensitive sensors of various molecules through the use of surface-enhanced Raman scattering. However, the method of manufacturing such nanostructures is often complex and difficult to reproduce. Herein, we report a simple, one-pot synthesis of flower-like gold nanostructures (AuNFs) in a porous Si/SiO2 matrix for potential surface enhanced Raman spectroscopy (SERS) applications. Templates covered with 500-nm diameter AuNFs with rough coral-like petals were obtained through a simple electroless reduction of gold(III) chloride salt in diluted hydrofluoric acid inside pores in the Si/SiO2 layer, which were obtained by swift heavy ion track technology. Detailed SERS analysis showed that single nanoflowers are capable of enhancing Raman signals and can be used as independent nanosensors. Having a few AuNFs in close proximity to each other enables more “hot spots” to be formed with an even greater increase in signal intensity. Using such substrates, reliable detection of 4-mercaptopyridine could be achieved down to 0.01 μM concentration. Finally, we theoretically demonstrate that the AuNFs are characterized by a wide-band plasmonic mode as well as by hot spots in the electromagnetic near-field distribution, which can result in a high enhancement factor for Raman scattering.
关键词： Surface-Enhanced Raman scattering,gold,nanoflowers,sensorics
更新于2025-09-11 14:15:04