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Multistage delivery of CDs-DOX/ICG-loaded liposome for highly penetration and effective chemo-photothermal combination therapy
摘要： Nanoparticles (NPs) have proven to be effective drug carriers in diagnosis and therapy of cancer. But, they faced a contradictory issue that NPs with large size appear weak tumor penetration, meanwhile small size resulted in poor tumor retention. Herein, we fabricated doxorubicin conjugated carbon dots (CDs-DOX) and indocyanine green (ICG)-loaded liposomes (ICG-LPs) named CDs-ICG-LPs using a modified reverse phase evaporation process, and with high incorporation in the aqueous core. The CDs-ICG-LPs exhibited good monodispersity, excellent fluorescence/size stability, and consistent spectra characteristics compared with free ICG or DOX. Moreover, the CDs-ICG-LPs showed higher temperature response, faster DOX release under laser irradiation. In the meantime, the fluorescence of DOX and ICG in CDs-ICG-LPs was also visualized for the process of subcellular location in vitro. In comparison with chemo or photothermal treatment alone, the combined treatment of CDs-ICG-LPs with laser irradiation synergistically induced the apoptosis and death of DOX-sensitive HepG2 cells. In vivo antitumor activities demonstrated CDs-ICG-LPs could reach higher antitumor activity compared with CDs-DOX and ICG-LPs for H22 tumor cells, and suppressed H22 tumor growth in vivo. Notably, no systemic toxicity occurrence was observed after repeated dose of CDs-ICG-LPs with laser irradiation. Hence, the well-defined CDs-ICG-LPs exhibited great potential in targeting cancer imaging and chemo-photothermal therapy.
关键词： highly penetration,indocyanine green,chemo-photothermal,doxorubicin,liposome,Carbon dots
更新于2025-09-09 09:28:46
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An update on inflammatory choroidal neovascularization: epidemiology, multimodal imaging, and management
摘要： Inflammatory choroidal neovascular membranes are challenging to diagnose and manage. A number of uveitic entities may be complicated by the development of choroidal neovascularization leading to a decrease in central visual acuity. In conditions such as punctate inner choroidopathy, development of choroidal neovascularization is extremely common and must be suspected in all cases. On the other hand, in patients with conditions such as serpiginous choroiditis, and multifocal choroiditis, it may be difficult to differentiate between inflammatory choroiditis lesions and choroidal neovascularization. Multimodal imaging analysis, including the recently introduced technology of optical coherence tomography angiography, greatly aid in the diagnosis and management of inflammatory choroidal neovascularization. Management of these neovascular membranes consists of anti-vascular growth factor agents, with or without concomitant anti-inflammatory and/or corticosteroid therapy.
关键词： Posterior uveitis,angiography,Choroiditis,Uveitis,Fluorescein angiography,Optical coherence tomography angiography,EDI-OCT,Indocyanine green,Inflammatory choroidal neovascularization
更新于2025-09-09 09:28:46
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The application of fluorescence techniques in meningioma surgery—a review
摘要： Surgical resections of meningiomas, the most common intracranial tumor in adults, can only be curative if radical resection is achieved. Potentially, the extent of resection could be improved, especially in complex and/or high-grade meningiomas by fluorescence-guided surgery using 5-aminolevulinic acid (5-ALA), indocyanine green (ICG), or fluorescein. This review aims to summarize and evaluate these fluorescence-guided meningioma surgery techniques. PubMed and Embase were searched for relevant articles. Additionally, we checked reference lists for further studies. Forty-eight articles were included in the final analysis. 5-ALA fluoresced with varying sensitivity and selectivity in meningiomas and in invaded bone and dura mater. Although ICG was mainly applied for video angiography, one report shows tumor fluorescence 18–28 h post-ICG injection. Lastly, the use of fluorescein could aid in the identification of tumor remnants; however, detection of dural tail is highly questionable. Fluorescence-guided meningioma surgery should be a reliable, highly specific, and sensitive technique. Despite numerous studies reporting the use of fluorescent dyes, currently, there is no evidence that these tools improve the radical resection rate and long-term recurrence-free outcome in meningioma surgery without neurological deficits. Evidence regarding the effectiveness and increased safety of resection after the application of these fluorophores is currently lacking. Future research should focus on the development of a meningioma-targeted, highly sensitive, and specific fluorophore.
关键词： Fluorescence-guided surgery,Meningioma,Indocyanine green,5-Aminolevulinic acid,Intraoperative imaging,Fluorescein
更新于2025-09-04 15:30:14
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Quantitative Assessment of the Blood Perfusion of the Gastric Conduit by Indocyanine Green Imaging
摘要： Background: Indocyanine green (ICG) ?uorescence imaging has been used to assess the blood perfusion of the gastrointestinal tract in surgery. Especially, it was used to determine the best anastomotic site. However, in previous studies, ICG ?uorescence was judged subjectively based on the visual appearance. This study evaluated the usefulness of our novel method to quantitatively assess the blood perfusion of the gastric conduit in esophagectomy based on ICG ?uorescence. Materials and methods: Twenty patients who underwent esophagectomy with gastric conduit reconstruction were prospectively investigated. Using a camera in contact with the surface of the stomach, ICG images were quantitatively evaluated using “ROIs”, a software program that quanti?ed the ?uorescence intensity and created a time-?uorescence intensity curve to assess the blood perfusion three times intraoperatively. Results: No postoperative esophago-gastrostomy leakage developed. However, after preparing the gastric conduit and just before anastomosis, the maximum increase in ?uorescence intensity (FImax) signi?cantly decreased (48.8 (cid:1) 26.0 and 31.5 (cid:1) 14.9 versus 84.9 (cid:1) 28.2; P < 0.001 and P < 0.001, respectively), and the time to reach FImax was signi?cantly extended (60 (cid:1) 35.4 and 58.3 (cid:1) 34.9 versus 18.9 (cid:1) 6.5; P < 0.001 and P < 0.002, respectively), in comparison to the control value. Just before anastomosis, 18 cases (90%) showed an identical pattern characterized by an obtuse and low arterial in?ow peak and a slow rise of ?uorescence intensity over time, indicating a decreased blood ?ow. Conclusions: The quantitative analysis of ICG ?uorescence imaging could objectively prove a decrease in blood perfusiondwhich could not be determined macroscopicallydin the gastric conduit before esophageal reconstruction. The results from the present and further studies may indicate its clinical value.
关键词： Gastric conduit,Anastomotic leakage,Indocyanine green,Esophagectomy,Blood perfusion,Quantitative assessment
更新于2025-09-04 15:30:14
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Indocyanine green‐enhanced fluorescence‐guided video‐assisted axillary surgery for chronic lymphocele
摘要： Introduction: Lymphocele occurs frequently after regional lymph node dissection. Surgery with multiple lymphatic ligation represents the only reliable treatment for long-standing chronic lymphocele. Materials and Surgical Technique: We report the case of a man with chronic axillary seroma after axillary lymphadenectomy for melanoma. The lymphocele was unresponsive to conservative management and successfully treated with indocyanine green lymphography-guided video-assisted axillary surgery. Discussion: Indocyanine green-enhanced fluorescence video-assisted identification and ligation of lymph vessels can simply and effectively treat recurrent chronic lymphocele after axillary or groin lymphadenectomy.
关键词： video-assisted surgery,lymphocele,Indocyanine green
更新于2025-09-04 15:30:14
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Near-infrared nanoparticles based on indocyanine green-conjugated albumin: a versatile platform for imaging-guided synergistic tumor chemo-phototherapy with temperature-responsive drug release
摘要： The aim of this study was to develop a multifunctional theranostic agent based on BSA nanoparticles (NPs), which loaded artemisinin (ART) and co-conjugated with indocyanine green (ICG) and arginine-glycine-aspartic acid (RGD) peptide (RGD-indocyanine green-Bovine Serum Albumin- artemisinin [IBA] NPs). The physicochemical parameters of RGD-IBA NPs were characterized in terms of the particle size, zeta potential, morphology, entrapment efficiency, drug loading, in vitro release behavior, photothermal and photodynamic effect, and in vitro anticancer ability. In vivo fluorescence and thermal imaging as well as antitumor studies were also evaluated. The tumor chemotherapeutic effects of ART and the ability of fluorescence imaging, hyperthermia generation and reactive oxygen species production of ICG and tumor-targeting RGD were integrated to achieve RGD-IBA NPs for imaging-guided tumor-targeted chemotherapy/photothermal/photodynamic therapy (chemo-phototherapy). The RGD-IBA NPs showed enhanced physiological stability and photo-stability compared with free ART and ICG. In addition, they were temperature-responsive; their sizes increased with increasing temperature between 25°C and 55°C, thereby leading to drug release upon the irradiation with near infrared (NIR) laser. In vivo fluorescence images of tumor-bearing mice showed that the RGD-IBA NPs could highly and passively reach the targeted tumor region with maximum accumulation at 24 hours post-intravenous injection. The in vitro and in vivo results demonstrated that the RGD-IBA NPs not only have good biocompatibility, but also are highly efficient tumor synergistic chemo-phototherapeutic agents. Through this study, it was found that RGD-IBA NPs could potentially be a very promising tumor theranostic agent.
关键词： chemo-phototherapy,indocyanine green,imaging-guided tumor therapy,artemisinin,theranostic
更新于2025-09-04 15:30:14
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Feasibility of indocyanine green fluorescence imaging for intraoperative identification of parathyroid glands during thyroid surgery
摘要： Background: This study assessed the feasibility of near-infrared fluorescence imaging with indocyanine green (ICG) to identify the parathyroid glands (PGs) intraoperatively and to assess their perfusion after thyroid resection. Methods: Patients undergoing elective thyroidectomy were enrolled in this prospective study. An intravenous bolus of 7.5 mg ICG was administered twice: the first bolus to identify the PGs before resection of the thyroid and the second to assess vascularization of the PGs after resection. Results: A total of 30 operations in 26 patients were included. In 17 surgeries (56.7%), fluorescence imaging was of added value, especially to confirm the presence of a suspected PG. No intraoperative or postoperative complications occurred because of the use of ICG. Conclusion: Near-infrared fluorescence imaging with the use of ICG for intraoperative identification of the PGs and the assessment of its vascularization is feasible and safe and can provide more certainty about the location of the PGs.
关键词： near-infrared fluorescence imaging,thyroidectomy,NIRF,indocyanine green,parathyroid gland
更新于2025-09-04 15:30:14
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Central serous chorioretinopathy in elderly subjects: angiographic and tomographic characteristics
摘要： Purpose To investigate the angiographic, tomographic, and clinical characteristics of idiopathic central serous chorioretinopathy (CSC) in elderly patients. Methods The patients were divided into two groups according to a cutoff age of 60 years at baseline. Patients underwent spectral domain optical coherence tomography, fluorescein angiography, and indocyanine green angiography. Angiographic and tomographic features were compared between the two groups (young vs. elderly group). Results Of 176 patients, 26 patients (15.1%) were 60 years or older. Complete resolution of subretinal fluid after treatment was noted in 72.0% of the elderly group and 90.8% of the young group (P = 0.021). The elderly group showed worse baseline and final vision, more bilateral involvement, and lower male preponderance than the young group (P < 0.05, respectively). The elderly group was also associated with a higher frequency of retinal pigment epithelium depigmentation, foveal thinning, and double-layer sign compared with the young group (P < 0.05, respectively). Conclusion CSC in elderly patients was associated with a lower resolution of serous detachment, increased impairment of retinal pigment epithelial layers, foveal thinning, and worse visual outcome, suggesting a chronic insult to the choroidal vessels involving more severe damage to the outer retinal layers.
关键词： Pachychoroid,Polypoidal choroidal vasculopathy,Fluorescein angiography,Photo dynamic therapy,Imaging,Optical coherence tomography,Indocyanine green angiography,Central serous chorioretinopathy
更新于2025-09-04 15:30:14