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A Simple Way to Simultaneously Release the Interface Stress and Realize the Inner Encapsulation for Highly Efficient and Stable Perovskite Solar Cells
摘要： Protein post-translational modification by the small ubiquitin-like modifier (SUMO) is a mechanism that allows a diverse response of cells to stress. Five SUMO family members, SUMO1?5, are expressed in mammals. We hypothesized that because kidney epithelial cells are often subject to stresses arising from various physiological conditions, multiple proteins in the kidney will be SUMOylated. Here, we profiled SUMO1- and SUMO2-modified proteins in a polarized epithelial cell model of the renal cortical collecting duct (mpkCCD14 cells). Modified forms of SUMO1 or SUMO2, with a histidine tag and a Thr to Lys mutation preceding the carboxyl-terminal di-gly motif, were expressed in mpkCCD14 cells, allowing SUMO-conjugated proteins to be purified and identified. Protein mass spectrometry identified 1428 SUMO1 and 1957 SUMO2 sites, corresponding to 741 SUMO1 and 971 SUMO2 proteins. Gene ontology indicated that the function of the majority of SUMOylated proteins in mpkCCD14 cells was related to gene transcription. After treatment of the mpkCCD14 cells for 24 h with aldosterone, the levels of SUMOylation at a specific site on the proton and oligopeptide/antibiotic cotransporter protein Pept2 were greatly increased. In conclusion, the SUMOylation landscape of mpkCCD14 cells suggests that protein modification by SUMOylation is a mechanism within renal epithelial cells to modulate gene transcription under various physiological conditions.
关键词： ion transport,post-translational modification,proteomics,kidney,epithelia
更新于2025-09-19 17:13:59
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Design and commissioning of an ion guide system for In-Gas Laser Ionization and Spectroscopy experiments
摘要： Radio-frequency (RF) ion guides, also known as Linear Paul Traps, are powerful devices to efficiently transport ion beams from high to low pressure regions while keeping good ion optical properties. A set of ion guides comprising three different RF quadrupole (RFQ) structures has been designed using ion-trajectory simulations to improve the performance of the In-Gas Laser Ionizations and Spectroscopy (IGLIS) technique currently under development at KU Leuven. Results of the commissioning tests for the total transport efficiency and transient time through the ion guides as well as the longitudinal energy spread and transverse emittance are found to be in agreement with ion trajectory simulations considering a realistic ion-atom interaction potential.
关键词： Radioactive ion beams,Ion transport,Radiofrequency quadrupole,Nuclear physics,Ion guide,Laser spectroscopy,Exotic nuclei
更新于2025-09-16 10:30:52
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Ion Transport in Laser-Induced Graphene Cation-Exchange Membrane Hybrids
摘要： Ion exchange membranes hybridized with laser-induced graphene (LIG) might lead to membranes with functional surface effects such as antifouling, antibacterial or joule heating, however, understanding the change in the electrical properties of the membrane is essential. Here we studied LIG-modified ion-exchange polymeric membranes using electrochemical impedance spectroscopy (EIS). The conductivity of the anionic sulfonated polyethersulfone membranes and the effective capacitance of the membrane-electrolyte interface were obtained by fitting the EIS spectra to an electrochemical equivalent circuit and compared to LIG-modified non-ionic polyethersulfone films. The transport selectivity (as relative permeability) of counter-ions (K+, Na+, Mg2+, Ca2+) across the membrane was quantified using the membrane’s conductivities obtained from the EIS measurements. The total ohmic resistance of the membrane was directly correlated to the polymer thickness, (with negligible contribution from the conductive LIG layer), thereby establishing EIS as a rapid, low-cost and non-invasive method to accurately probe substrate usage in LIG modification.
关键词： laser-induced graphene,electrochemical impedance spectroscopy,capacitance,ion exchange membranes,conductivity,ion transport
更新于2025-09-16 10:30:52
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Synthesis of carbon quantum dots in a Nafion matrix: Precursor effect on the ion transport properties
摘要： Carbon quantum dots have been prepared in a Nafion? matrix via the hydrothermal treatment of organic precursors, and the swelling and ion transport properties of the prepared composite materials have been characterized.
关键词： Nafion matrix,ion transport properties,carbon quantum dots,hydrothermal treatment
更新于2025-09-16 10:30:52