- 标题
- 摘要
- 关键词
- 实验方案
- 产品
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Laser Scanning Confocal Microscopy 3D Surface Metrology Applications
摘要： Surface metrology, measurement of solid surfaces topography, has become an important topic for many material scientists and engineers. Characterization of the surfaces can help researchers find new functional materials, improve device performance and so forth. ‘Seeing is believing.’ Visualization of fine 3-dimensional (3D) details of surfaces is critical in surface metrology studies. Among various observation and measurement techniques, Laser Scanning Confocal Microscopy (LSCM) becomes more and more popular due to the fact that it is non-contact and non-destructive to the samples, requires minimal sample preparation, and efficient automations and provides single nanometer level resolution. In this paper, we will introduce these features in detail, by using Olympus LEXT OLS5000, the newest 3D laser confocal scanning microscope.
关键词： Olympus LEXT OLS5000,3D Surface Metrology,Laser Scanning Confocal Microscopy
更新于2025-09-23 15:19:57
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Terrestrial laser scanninga??derived canopy interception index for predicting rainfall interception
摘要： Rainfall interception (RI) by forest canopies is an important process in hydrological cycling in forest ecosystems. However, accurately predicting RI is a challenging topic. In this study, a dimensionless descriptor, canopy interception index (CII), for predicting RI was defined. The terrestrial laser scanning was used to estimate CII in four temperate forest types, including Korean pine (Pinus koraiensis) plantation forest (KPF) stands, larch (Larix spp.) plantation forest (LPF) stands, mixed broadleaved forest (MBF) stands and Mongolian oak (Quercus mongolica) forest (MOF) stands. Using the measured RI values over the rainy seasons in 2017 and 2018, CII’s performance for predicting RI was tested, and also compared with several other indices (LAI: leaf area index, PAI: plant area index and ACH: average canopy height). The results indicated that CII was significantly and strongly related with RI for the four forest types together (R2 = 0.79), as well as for an individual forest type (R2 = 0.55~0.63). More importantly, its performance was better than those from LAI (R2 = 0.33~0.43), PAI (R2 = 0.40~0.53) and ACH (R2 = 0.35). All those results demonstrated that CII was an efficient index for accurately predicting RI. The potential applications of CII were also discussed.
关键词： dimensionless descriptor,terrestrial laser scanning,temperate forests,canopy interception index,rainfall interception
更新于2025-09-23 15:19:57
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[Developments in Earth Surface Processes] Remote Sensing of Geomorphology Volume 23 || Landslide analysis using laser scanners
摘要： The advent of light detection and ranging (LiDAR) (see Table 1 for acronyms) has revolutionized the study of landslides and geomorphology because it provides an extraordinarily fine resolution topography (Carter et al., 2001). Electronic components and computers at affordable prices have made this technique widely available since the beginning of the 21st century. The number of geoscientific publications is increasing at a nearly exponential rate since around 1990 demonstrating its impact on geosciences (Abellan et al., 2016).
关键词： LiDAR,landslides,geomorphology,topography,laser scanning
更新于2025-09-23 15:19:57
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Digital Modeling and Display of Ancient Architecture Based on Multi-Station Laser Scanning
摘要： In order to better display and protect ancient Chinese architecture, a three-dimensional model reconstruction method based on multi-station laser scanning is proposed. This method mainly includes several steps, such as point cloud data collection, preconditioning, multi-site cloud data fusion, point cloud data compression, 3D model reconstruction and texture mapping, environment rendering, video processing, and 3D roaming. According to the requirement of video rendering and virtual roaming, we focus on cloud processing, 3D modeling and 3D model display in this paper. Experimental results show that an famous building named Enshi Dong Drum Tower is well digitally reconstructed, so as to result in ethnic characteristics and cultural heritage protection in practical application.
关键词： 3D laser scanning,3D reconstruction,ancient architecture,point cloud data,digital modeling
更新于2025-09-23 15:19:57
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[IEEE 2019 2nd World Conference on Mechanical Engineering and Intelligent Manufacturing (WCMEIM) - Shanghai, China (2019.11.22-2019.11.24)] 2019 2nd World Conference on Mechanical Engineering and Intelligent Manufacturing (WCMEIM) - Deep Sea Laser Scanning System for Underwater In-Situ Measurement
摘要： In this paper, a novel laser scanning system for deep sea in-situ measurement was developed. Firstly, the main components of the prototype were introduced separately. Then, the performance of the system was tested and analyzed in a tank filled with water with a standard ball bar. Results showed that the laser scanning system in water can achieve high precision. In addition, the prototype was placed in deep sea to verify the performance of the in-situ measurement and the measured targets are well reconstructed.
关键词： high precision,laser scanning system,in-situ measurement,deep sea
更新于2025-09-23 15:19:57
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Editorial: Advances in Label Free Tissue Imaging With Laser Scanning Microscopy Techniques
摘要： Significant efforts are being spent at the time being for transferring various laser scanning microscopy (LSM) techniques to the realm of tissue characterization, because of their potential to circumvent some of the most important disadvantages of traditional histopathology approaches based on excisional biopsy and tissue staining. Although conventional histopathology is currently regarded as a golden standard for the diagnosing pathologies that reflect in tissular modification (e.g., cancers), limitations such as long diagnosis time, invasiveness, artifacts, sampling error, time consumption, high costs, and interpretive variability make such approaches to be impractical in many scenarios, while also placing considerable pressure on the sustainability of healthcare systems around the world. The potential of LSM techniques to contribute to overcoming these aspects derives from their “non-invasive” character. They can exploit various endogenous optical signals generated by tissues upon interaction with a laser beam and are able to provide optical sections (virtual biopsies) that reflect the tissular architecture at controlled depths. Many studies reported to date showed that LSM techniques can provide label-free information of similar pathologic relevance to the information collected for characterization/confirmation purposes with traditional histopathology approaches. These techniques are thus capable of probing optical properties of tissues with deep implications for resolving important anatomical and physiological aspects which represent hallmarks for disease predisposition and progression. To date techniques such as Confocal Laser Scanning Microscopy (CLSM) [1], Fluorescence Lifetime Imaging (FLIM) [2], Two-Photon Excited Fluorescence Microscopy (TPEF) [2–6], Second Harmonic Generation Microscopy (SHG) [5, 6], Third Harmonic Generation Microscopy (THG) [4], Coherent Anti-Stokes Raman Scattering Microscopy (CARS) [3, 7], as well as other LSM variants such as the Brillouin Microscopy [8] have already been demonstrated to be powerful tools for investigating tissue morphology, functionality, and biochemical composition with high spatial and temporal resolution. In the opinion of many, these techniques, together with investigations approaches based on their combined use, will soon become the central element of the default tissue characterization frameworks for both ex vivo and in vivo assays. Furthermore, emerging LSM techniques exploiting various ingenious strategies to achieve superresolved images in a label-free manner [9–12] are also likely to be transferred soon toward applications addressing tissue imaging.
关键词： tissue imaging,two photon excitation fluorescence microscopy,Brillouin microspectroscopy,laser scanning microscopy,second harmonic generation microscopy
更新于2025-09-23 15:19:57
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Assessment of a house affected by ground movement using terrestrial laser scanning and numerical modeling
摘要： This study analyzed the internal mechanical state of houses disturbed by underground coal mining, especially those that are free from damage in subsidence areas. Traditional surveying and terrestrial laser scanning (TLS) were utilized as the external monitoring methods to obtain the outside displacement of the disturbed houses. The results were then used to estimate the internal mechanical state of houses using simulation software. Three high-quality houses in Paifang Village, China were scanned using TLS in their pre- and post-mining stages. The magnitude of movement and deformation was highly related to the location of the building in the panel, which was enlarged near the center of the subsidence basin. ANSYS was used to compute the internal state of the houses with a focus on the structural columns, ring beams, and walls of the houses under changing tensile conditions. Results showed that the structural column and ring beam can resist tensile deformation, whereas the window corner was the principal point of stress concentration with potential risk of cracking. Mastering the internal stress state of undamaged buildings in mining-affected areas can help to strengthen the premaintenance of houses and guide the construction of new anti-deformation houses. The results of this work may benefit the construction of anti-deformation buildings in mining regions.
关键词： Underground coal mine,Internal mechanical state of houses,Terrestrial laser scanning,Numerical simulation,Traditional surveying
更新于2025-09-23 15:19:57
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Characterization of Nanoencapsulated Food Ingredients || Confocal laser scanning microscopy (CLSM) of nanoencapsulated food ingredients
摘要： Encapsulation is a process of entrapping bioactive compounds (e.g., antioxidants, antimicrobials, essential fatty acids, probiotics, vitamins, etc.) within a protecting wall material such as lipid-based or biopolymeric carriers. Encapsulation can help to enhance the stability and bioaccessibility as well as controlled release of bioactive materials and mask unwanted properties of encapsulated ingredients. Nanoparticles have a diameter range from 0.1 nm to 1 μm and show great potential to develop new products and applications in food and packaging industries. The performance of nanocarriers formed by encapsulation processes fundamentally depends on various technical aspects such as carrier wall thickness, morphology, and quality. Therefore, measuring and monitoring of coating thickness and morphology of the nanocarriers is an essential process to control the nanoencapsulation procedure. An excessively thick coating layer would lead to delayed release of bioactive materials, increase of coating time, and consequently, encapsulation costs, while an excessively thin capsule would cause interruption in the release and stability of nanoparticles. Spectroscopic and microscopic techniques are important tools for analyzing the morphology, structure, and coating thickness of nanoparticles. Different electron microscopies have been introduced to investigate nano- and micro-carriers. Scanning electronic microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), near-IR spectra, laser-induced breakdown spectroscopy (LIBS), and confocal laser scanning microscopy (CLSM) are some of these light and microscopic methods.
关键词： Encapsulation,Nanoencapsulated food ingredients,Confocal laser scanning microscopy,Bioactive compounds,Nanoparticles
更新于2025-09-23 15:19:57
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The effect of different obturation methods on sealer penetration alongside apically separated rotary nickela??titanium instruments: A confocal laser scanning microscopy study
摘要： The effects of different obturation techniques on calcium silicate-based sealer penetration in the presence of apically separated rotary files were evaluated. Forty-eight extracted mandibular incisors were used. ProTaper F2 rotary files were separated at the apical thirds. Samples were divided into four groups (n = 12) according to obturation technique used: (a) cold lateral compaction (CLC); (b) single cone; (c) bulk-fill (BF) without a core material; and (d) thermoplastic injection (TI). Specimens were sectioned horizontally at 1 and 3 mm from the apex and studied using a confocal scanning laser microscope. The maximum tubule penetration depth and percentage of penetration were measured. Data were statistically analyzed using parametric and nonparametric tests with a significance level of 5%. Regarding penetration depth, a significant difference was found at 1 mm (p < .05), while no significant difference was found at 3 mm (p > .05). At the 3 mm level, all of the obturation techniques showed similar penetration depths. Regarding penetration percentage, the values of the CLC and TI groups were statistically less when compared with the BF group at 1 and 3 mm levels, respectively (p < .05). Under the limitations of this in vitro study, results suggest that the obturation technique may present a significant effect on sealer penetration.
关键词： sealer penetration,separated instruments,obturation technique,confocal laser scanning microscope
更新于2025-09-23 15:19:57
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Under-canopy UAV laser scanning for accurate forest field measurements
摘要： Surveying and robotic technologies are converging, offering great potential for robotic-assisted data collection and support for labour intensive surveying activities. From a forest monitoring perspective, there are several technological and operational aspects to address concerning under-canopy flying unmanned airborne vehicles (UAV). To demonstrate this emerging technology, we investigated tree detection and stem curve estimation using laser scanning data obtained with an under-canopy flying UAV. To this end, we mounted a Kaarta Stencil-1 laser scanner with an integrated simultaneous localization and mapping (SLAM) system on board an UAV that was manually piloted with the help of video goggles receiving a live video feed from the onboard camera of the UAV. Using the under-canopy flying UAV, we collected SLAM-corrected point cloud data in a boreal forest on two 32 m 32 m test sites that were characterized as sparse ( = 42 trees) and obstructed ( = 43 trees), respectively. Novel data processing algorithms were applied for the point clouds in order to detect the stems of individual trees and to extract their stem curves and diameters at breast height (DBH). The estimated tree attributes were compared against highly accurate field reference data that was acquired semi-manually with a multi-scan terrestrial laser scanner (TLS). The proposed method succeeded in detecting 93% of the stems in the sparse plot and 84% of the stems in the obstructed plot. In the sparse plot, the DBH and stem curve estimates had a root-mean-squared error (RMSE) of 0.60 cm (2.2%) and 1.2 cm (5.0%), respectively, whereas the corresponding values for the obstructed plot were 0.92 cm (3.1%) and 1.4 cm (5.2%). By combining the stem curves extracted from the under-canopy UAV laser scanning data with tree heights derived from above-canopy UAV laser scanning data, we computed stem volumes for the detected trees with a relative RMSE of 10.1% in both plots. Thus, the combination of under-canopy and above-canopy UAV laser scanning allowed us to extract the stem volumes with an accuracy comparable to the past best studies based on TLS in boreal forest conditions. Since the stems of several spruces located on the test sites suffered from severe occlusion and could not be detected with the stem-based method, we developed a separate work flow capable of detecting trees with occluded stems. The proposed work flow enabled us to detect 98% of trees in the sparse plot and 93% of the trees in the obstructed plot with a 100% correction level in both plots. A key benefit provided by the under-canopy UAV laser scanner is the short period of time required for data collection, currently demonstrated to be much faster than the time required for field measurements and TLS. The quality of the measurements acquired with the under-canopy flying UAV combined with the demonstrated efficiency indicates operational potential for supporting fast and accurate forest resource inventories.
关键词： Stem volume,Under-canopy flight,SLAM,Airborne laser scanning,Stem curve,UAV
更新于2025-09-23 15:19:57