- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Photodynamic Therapy With Bengal Rose and Derivatives Against Leishmania amazonensis
摘要： Introduction: The treatment of cutaneous leishmaniasis (CL) is based primarily on the use of pentavalent antimonials, which may lead to many side effects limiting their use. Photodynamic therapy (PDT) is an alternative for the treatment of CL, and some xanthene dyes have the potential for use in PDT. Methods: The xanthenes rose bengal B (RB) and its derivatives rose bengal methyl ester (RBMET), and butyl ester (RBBUT) were analyzed for leishmanicidal activity against promastigotes and intracellular amastigotes of Leishmania amazonensis. Cytotoxicity was assessed in J774.A1 macrophages. Results: RB derivates RBMET (IC50 9.83 μM), and RBBUT (IC50 45.08 μM) showed leishmanicidal activity, however, were toxic to J774.A1 macrophages, resulting in low selectivity index. Conclusion: The RBMET and RBBUT showed to be effective against the L. amazonensis and the low selectivity index presented may not be a limitation for their use in PDT to CL treatment.
关键词： Photodynamic therapy,Xanthenes,Rose Bengal B,Leishmania amazonensis,Cutaneous leishmaniasis
更新于2025-09-23 15:22:29
-
Application of laser for treatment of cutaneous leishmaniasis: a review of literature
摘要： Cutaneous leishmaniasis (CL) is a major health problem in developing countries with high economic and health impact. Despite suggested treatment for CL, there is still no definite therapy for this infection, and many of these treatments are associated with serious local and systemic side effects. In the current paper, use of different laser types including continuous and fractional CO2, argon, PDL, erbium glass, and Nd:YAG have been reviewed. Based on our review, given the high reported efficacy and low side-effect profile, use of laser can be considered as a good alternative to standard treatment of cutaneous leishmaniasis (CL). Performing more studies using different types of lasers is recommended to evaluate the efficacy of this method for treatment of CL.
关键词： Treatment,Laser,Cutaneous leishmaniasis
更新于2025-09-23 15:19:57
-
Development of plasmonic ELISA for the detection of anti-Leishmania sp. IgG antibodies
摘要： Recently, a novel Enzyme-Linked Immunosorbent Assay (ELISA) strategy has emerged, known as “plasmonic ELISA” (pELISA), which enables the detection of disease biomarkers at low concentrations with the naked eye. For the ?rst time, this research has developed a signal-generation mechanism for the detection of anti-Leishmania sp. IgG antibodies with the naked eye using pELISA. The immunoassay incorporates an indirect ELISA with successive growth of gold nanoparticles to obtain blue or red-colored solutions in the presence or absence of anti-Leishmania sp. IgG antibodies in canine serum, respectively. The technique we developed was successfully tested in canine serum positive and negative for canine leishmaniasis (CanL), and was shown to be an e?ective method that could be used as an additional tool for CanL diagnosis. It will be particularly useful in resource-constrained countries, because it does not require sophisticated instruments to read the results, increasing the practicality of CanL detection in these areas.
关键词： Canine Leishmaniasis,Metal nanoparticles,Enzyme-linked immunosorbent assay
更新于2025-09-12 10:27:22
-
Combination of continuous wave CO <sub/>2</sub> laser and topical application of trichloroacetic acid 50% vs CO <sub/>2</sub> laser alone the treatment of cutaneous leishmaniasis (A case series of 6 patients)
摘要： Background: Cutaneous leishmaniasis (CL) is an endemic disease of Iran and despite self‐healing nature of this disease and many suggested treatments, scar is usually inevitable results of the affection with this cutaneous infection. CO2 laser in CL has a specific thermolysis effect on infected tissues and application of trichloroacetic acid (TCA) 50% solution for treatment of leishmaniasis scars showed promising results. Aim: To evaluate CO2 laser plus TCA 50% versus CO2 laser alone for treatment of CL. Patients/Methods: In the current report, we present 6 cases of noduloulcerative CL that were treated successfully by the combination of the continuous wave CO2 laser plus topical application of TCA 50%. Results: Five patients improved after 3 weeks of starting treatment, and 1 patient needed to repeat treatment. The scars were flat and good. Conclusions: The CO2 laser plus topical TCA 50% may be more effective in treatment of the cutaneous leishmaniasis and results in shorter healing time.
关键词： treatment,trichloroacetic acid,leishmaniasis,laser,CO2 laser
更新于2025-09-11 14:15:04