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Pulsed interleaved excitation-based line-scanning spatial correlation spectroscopy (PIE-lsSCS)
摘要： We report pulsed interleaved excitation (PIE) based line-scanning spatial correlation spectroscopy (PIE-lsSCS), a quantitative fluorescence microscopy method for the study of dynamics in free-standing lipid bilayer membranes. Using a confocal microscope, we scan multiple lines perpendicularly through the membrane, each one laterally displaced from the previous one by several ten nanometers. Scanning through the membrane enables us to eliminate intensity fluctuations due to membrane displacements with respect to the observation volume. The diffusion of fluorescent molecules within the membrane is quantified by spatial correlation analysis, based on the fixed lag times between successive line scans. PIE affords dual-color excitation within a single line scan and avoids channel crosstalk. PIE-lsSCS data are acquired from a larger membrane region so that sampling is more efficient. Moreover, the local photon flux is reduced compared with single-point experiments, resulting in a smaller fraction of photobleached molecules for identical exposure times. This is helpful for precise measurements on live cells and tissues. We have evaluated the method with experiments on fluorescently labeled giant unilamellar vesicles (GUVs) and membrane-stained live cells.
关键词： lipid bilayer membranes,dual-color excitation,live cells,line-scanning spatial correlation spectroscopy,fluorescence microscopy,tissues,GUVs,confocal microscope,photobleaching,Pulsed interleaved excitation
更新于2025-09-23 15:21:01
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IR laser line scanning treatments to improve levitation forces in MgTi0.06B2 bulk materials
摘要： Infrared (IR) laser-line scanning has been widely used to induce different surface microstructures in a broad range of materials. In this work, this laser configuration was applied on the surface of MgTi0.06B2 bulk samples in order to ascertain its effects on their superconducting properties, particularly on the magnetic levitation forces. The microstructural changes produced by this type of laser treatment were investigated by X-ray diffraction (XRD) and Scanning Electron Microscopy (SEM). It was observed that the thermal treatment induced by the laser improves grain connectivity in a layer of material just below the irradiated surface, in agreement with the observed improvement in critical current density values, Jc, which were estimated from isothermal magnetic hysteresis loops. A significant increment of both vertical (Fz) and lateral (Fx) magnetic levitation forces was achieved. Numerical calculations were performed to understand the experimental behaviour and to clarify how an improvement of Jc near the surface can improve the magnetic levitation force of these materials. In addition, the same studies were carried out in similar bulk samples but with nano-sized silver particle additions of 3 and 6 wt %, in the outer ring of the bulk, observing also an improvement of the levitation forces, albeit less than in samples without Ag because of the better performance of the original samples after laser treatment. These results are relevant to those studying superconductor fabrication and material fabrication modelling, essential for the development of technological applications of superconductors, and are based on microstructure control via application of a recently developed laser-line scan method.
关键词： MgTi0.06B2,Critical current,Magnetic levitation force,IR laser-line scanning
更新于2025-09-12 10:27:22
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Femtosecond Laser Inscribed Sapphire Fiber Bragg Grating for High Temperature and Strain Sensing
摘要： In this paper, a sapphire fiber Bragg grating (SFBG) is fabricated by femtosecond (fs) laser line-by-line scanning method. A third-order fiber Bragg grating is obtained in a single crystal sapphire fiber with a diameter of 60 μm. Compared to the SFBG written by point-by-point method, the SFBG written by the line-by-line method has a higher reflectivity and grating with about 15% reflectivity is achieved when the track length is about 40 μm. The temperature sensing characteristics of this SFBG from room temperature to 1600°C are studied, and the temperature sensitivity of SFBG is 34.96 pm/°C from 1000°C to 1600°C. The strain characteristics are also tested at the temperature of 26°C, 500°C, 1000°C and 1600°C, and the strain sensitivities are 1.42 pm/με, 1.42pm/με, 1.44 pm/με and 1.45 pm/με, respectively. These characteristics show that this SFBG has potential applications in harsh environment structural health monitoring.
关键词： structural health monitoring,femtosecond laser,line-by-line scanning,Sapphire fiber Bragg grating
更新于2025-09-11 14:15:04
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Monitoring circulating tumor cells in vivo by a confocal microscopy system
摘要： Circulating tumor cells (CTCs) play a key role in cancer metastasis but are very difficult to detect. in vivo monitoring CTCs has been recognized as an important technique for cancer research and clinical diagnosis. Recently, a noninvasive method, in vivo flow cytometry (IVFC) has been developed to enable continuous, real-time, and long-duration detection of CTCs in animal models by detecting CTC fluorescence in blood vessels excited by lasers. In this study, we present a simple optical scheme for direct noninvasive CTC detection using confocal microscopes. We demonstrate that line scanning of confocal microscopy can provide effective and quantitative CTC detection in live mice during cancer development. Rare CTC signals can be acquired at the early stage of the tumor development after implantation of subcutaneous tumor and monitored continuously to the end. Signals from CTC clusters can also be acquired and distinguished from single CTCs. Our results suggest confocal microscopy is a simple and reliable method for biologists and doctors to use for cancer research.
关键词： line scanning,circulating tumor cells,confocal microscopy,CTC clusters
更新于2025-09-04 15:30:14