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过滤筛选
- 2018
- electromagnetic pulse
- cell proliferation
- cell membrane permeability
- cell response to electromagnetic stress
- apoptosis
- cancer therapy
- necrosis
- Intelligent Medical Engineering
- V.N. Karazin Kharkiv National University
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MORE EFFECTIVE SCREENING FOR EPIRETINAL MEMBRANES WITH MULTICOLOR SCANNING LASER OPHTHALMOSCOPE THAN WITH COLOR FUNDUS PHOTOGRAPHS
摘要： Purpose: To assess the ability of the multicolor scanning laser ophthalmoscope (MC-SLO) to screen for epiretinal membranes (ERMs). Methods: A retrospective cross-sectional study of 35 eyes of 32 patients with an ERM detected by optical coherence tomography and 46 eyes of 23 healthy volunteers. The detection of the ERM was graded into three visibility scores—1, not visible, 2, barely visible, and 3, clearly visible—by retina specialists or by ophthalmology residents. The sensitivity and speci?city of the detection with the merged image of the MC-SLO or color fundus photographs (CFPs) were calculated. Results: The sensitivity for ERM detection in the MC-SLO and CFP were 91.4% and 65.7% by specialists and 97.1% and 60.0% by residents. The speci?city for both devices was 100% by specialists and residents. The visibility score for the MC-SLO images were signi?cantly higher than that for the CFP by both specialists and residents. In addition, the visibility score for the MC-SLO determined by residents was signi?cantly higher than that for the CFP by specialists. Conclusion: The detection of an ERM is better with the MC-SLO images than with CFP. Furthermore, the ERM detection in the MC-SLO images by residents was comparable to that by specialists.
关键词： epiretinal membrane,multicolor scanning laser ophthalmoscope
更新于2025-09-12 10:27:22
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EFFECT OF LASER PHOTOCOAGULATION ON MACULAR EDEMA ASSOCIATED WITH MACULAR HOLES
摘要： To report the outcomes of laser therapy to barricade eccentric full-thickness macular hole with associated cystoid macular edema. We report two patients who developed an eccentric full-thickness macular hole with persistent cystoid macular edema after pars plan vitrectomy with and without internal limiting membrane peel for epiretinal membrane and the results of argon laser therapy. Barricade argon laser therapy was applied concentric to the full-thickness macular hole. Associated cystoid macular edema was noted to resolve within 1 to 3 months of therapy in both cases. Barricade laser therapy surrounding a macular hole can lead to resolution of associated cystoid macular edema. Pathogenic mechanisms to explain this favorable outcome are discussed.
关键词： cystoid macular edema,argon laser,epiretinal membrane,PPV/ILM,laser retinopexy,laser barricade,eccentric macular hole,macular hole
更新于2025-09-12 10:27:22
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A novel shaped-controlled fabrication of nanopore and its applications in quantum electronics
摘要： High-intensity (107–108 A m?2) electron beams can be used to fabricate nanoscale pores. this approach enables real-time observation of nanopore drilling and precise control of the diameter of the nanopore. nevertheless, it is not suitable for tuning the nanopore’s sidewall shape. in this study, we demonstrate the use of low-intensity electron beams to fabricate nanopores on a silicon nitride (Sinx) membrane. this technique allows the precise adjustment of the nanopore dimension and the shaping of its three-dimensional (3D) nanostructure. The 3D structures of the nanopore were evaluated by electron tomography, and series of oblique images were used in reconstructing the 3D images of nanopores using a weighted back-projection method. the sidewall shape of the nanopore was observed at different electron-beam conditions, and the formation mechanism was elucidated based on these results. the nanopore fabricated with this technique can be used as a template to develop electronics at the nanoscale based on which a quantum-dot device can be prepared with a simple evaporation process. The measured results show that the device can resolve well-defined electronic states that are characteristic for the behaviors of the quantum-dot device.
关键词： electron beam,nanopore,3D nanostructure,quantum electronics,silicon nitride membrane
更新于2025-09-12 10:27:22
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Model-based analysis of highly dynamic laser beam shaping using deformable mirrors
摘要： Deformable mirrors show large potential for the dynamic beam shaping in high power laser applications. A model which is based on influence functions is used to systematically investigate the beam shaping capabilities of several deformable mirrors based on the underlying technology and the number of actuators.
关键词： deformable mirror,dynamic beam shaping,high power,influence functions,piezoelectric mirror,membrane mirror
更新于2025-09-12 10:27:22
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[IEEE 2019 Compound Semiconductor Week (CSW) - Nara, Japan (2019.5.19-2019.5.23)] 2019 Compound Semiconductor Week (CSW) - InP membrane lasers and active-passive integration
摘要： In this paper we review the recent developments in the nanophotonic platform IMOS. High-performance optical amplifier based on twin-guide active-passive integration scheme has been demonstrated, enabling continuous wave operated lasers on IMOS. Future work will seek to integrate more advanced components, such as >67GHz detectors, polarization controllers and optical modulators in the same membrane.
关键词： laser,photonic integration,photonic membrane
更新于2025-09-12 10:27:22
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Development of Ratiometric Fluorescence Sensors Based on CdSe/ZnS Quantum Dots for the Detection of Hydrogen Peroxide
摘要： In this study, carboxyl group functionalized-CdSe/ZnS quantum dots (QDs) and aminofluorescein (AF)-encapsulated polymer particles were synthesized and immobilized to a sol–gel mixture of glycidoxypropyl trimethoxysilane (GPTMS) and aminopropyl trimethoxysilane (APTMS) for the fabrication of a hydrogen peroxide-sensing membrane. CdSe/ZnS QDs were used for the redox reaction of hydrogen peroxide (H2O2) via a reductive pathway by transferring electrons to the acceptor that led to fluorescence quenching of QDs, while AF was used as a reference dye. Herein, the ratiometric fluorescence intensity of CdSe/ZnS QDs and AF was proportional to the concentration of hydrogen peroxide. The fluorescence membrane (i.e., QD–AF membrane) could detect hydrogen peroxide in linear detection ranges from 0.1 to 1.0 mM with a detection limit (LOD) of 0.016 mM and from 1.0 to 10 mM with an LOD of 0.058 mM. The sensitivity of the QD–AF membrane was increased by immobilizing horseradish peroxidase (HRP) over the surface of the QD–AF membrane (i.e., HRP–QD–AF membrane). The HRP–QD–AF membrane had an LOD of 0.011 mM for 0.1–1 mM H2O2 and an LOD of 0.068 mM for 1–10 mM H2O2. It showed higher sensitivity than the QD–AF membrane only, although both membranes had good selectivity. The HRP–QD–AF membrane could be applied to determine the concentration of hydrogen peroxide in wastewater, while the QD–AF membrane could be employed for the detection of α-ketobutyrate.
关键词： ratiometric fluorescence QD membrane,CdSe/ZnS QDs,hydrogen peroxide,α-ketobutyrate,redox reaction
更新于2025-09-12 10:27:22
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Photocatalytic filtration reactors equipped with bi-plasmonic nanocomposite/poly acrylic acid-modified polyamide membranes for industrial wastewater treatment
摘要： In this study, two new composite membranes with antifouling and anti-biofouling properties were prepared through the modification of commercial polyamide (PA) discs using combination of in-situ polymerization of polyacrylic acid (PAA) and grafting of two synthesized bi-plasmonic Au-Ag and Ag-Au photocatalysts. The synthesis and characterization of the photocatalysts in batch mode were discussed in details as primary studies. Two intense 405-nm and 532-nm lasers for Ag-Au and Au-Ag photocatalysts, respectively and a solar-simulated xenon lamp for both photocatalysts were applied for photodegradation studies and the results were compared. In addition, the effect of other parameters such as type and amount of photocatalysts, and initial concentration of pollutants on the degradation efficiency of ofloxacin (OFX) and methylene blue (MB) as the model pollutant drug and dye were comprehensively investigated and the Langmuir-Hinshelwood adsorption model was used for evaluation of kinetics, degradation rate and half-life time of the reactions. After selection of xenon lamp as the optimum light source, the photodegradation of OFX and MB was evaluated in a dead-end membrane reactor (MR) and flux performance, antifouling property and pollutant removal of the membranes were evaluated using pharmaceutical and textile wastewater samples. In addition, the antibacterial activity of the prepared membranes was evaluated using Gram-negative E. coli bacteria as the model microorganism using thin film assay and Kirby-Bauer disk diffusion methods to examine the anti-biofouling potential of the constructed reactors. It was demonstrated that the prepared MR is able to produce cleaner water with more stable ?ux performance and good membrane fouling/biofouling properties in energy saving manner with respect to the unmodified ones.
关键词： Membrane ?ltration,Wastewater treatment,Permeate ?ux,Bi-plasmonic nanoparticles,Photodegradation,Antifouling/anti-biofouling properties
更新于2025-09-11 14:15:04
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Two-dimensional plasmonic biosensing platform: Cellular activity detection under laser stimulation
摘要： Combining biosensors with nanoscience provides great advantages such as being label-free and real time, highly sensitive, and small in size, as well as providing a low limit of detection and integration to other systems. That is why plasmonics finds various applications in drug detection, food safety, agriculture, photothermal therapy, etc. In this paper, we have fabricated a two-dimensional plasmonic grating biosensor using a soft lithography technique, which has eliminated some disadvantages of conventional plasmonic structures like expensive fabrication cost, inflexibility, and lack of mass production. On the other hand, we benefited from infrared neural stimulation for regulating membrane depolarization, which was based on photothermal mechanism and provided a contact-free and high spatial/temporal resolution. Eventually, the membrane depolarization of two different cell types of Hep G2 and mesenchymal stem cells cultured on two-dimensional plasmonic structure has been investigated under infrared neural stimulation. After preparing the soft plasmonic crystal, its reflection spectra and respective ellipsometry parameters were analyzed before and after cell culture with/without stimulation (near-infrared immune region ～1450 nm). By comparing the obtained ellipsometry results for HEP G2 and mesenchymal stem cells, it is observed that the behavior of two cell types with respect to IR stimulation was the same as well as providing us the possibility of distinguishing the level of membrane depolarization under various stimulating frequencies. The strength of this integrated system for membrane depolarization detection has been shown experimentally, which can open new avenues toward neuroplasmonic application in the future.
关键词： infrared neural stimulation,membrane depolarization,ellipsometry,soft lithography,plasmonic biosensor
更新于2025-09-11 14:15:04
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Sulfolobus acidocaldarius Microvesicles Exhibit Unusually Tight Packing Properties as Revealed by Optical Spectroscopy
摘要： In this study, we used optical spectroscopy to characterize the physical properties of microvesicles released from the thermoacidophilic archaeon Sulfolobus acidocaldarius (Sa-MVs). The most abundant proteins in Sa-MVs are the S-layer proteins, which self-assemble on the vesicle surface forming an array of crystalline structures. Lipids in Sa-MVs are exclusively bipolar tetraethers. We found that when excited at 275 nm, intrinsic protein fluorescence of Sa-MVs at 23 °C has an emission maximum at 303 nm (or 296 nm measured at 75 °C), which is unusually low for protein samples containing multiple tryptophans and tyrosines. In the presence of 10–11 mM of the surfactant n-tetradecyl-β-d-maltoside (TDM), Sa-MVs were disintegrated, the emission maximum of intrinsic protein fluorescence was shifted to 312 nm, and the excitation maximum was changed from 288 nm to 280.5 nm, in conjunction with a significant decrease (>2 times) in excitation band sharpness. These data suggest that most of the fluorescent amino acid residues in native Sa-MVs are in a tightly packed protein matrix and that the S-layer proteins may form J-aggregates. The membranes in Sa-MVs, as well as those of unilamellar vesicles (LUVs) made of the polar lipid fraction E (PLFE) tetraether lipids isolated from S. acidocaldarius (LUVPLFE), LUVs reconstituted from the tetraether lipids extracted from Sa-MVs (LUVMV) and LUVs made of the diester lipids, were investigated using the probe 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan). The generalized polarization (GP) values of Laurdan in tightly packed Sa-MVs, LUVMV, and LUVPLFE were found to be much lower than those obtained from less tightly packed DPPC gel state, which echoes the previous finding that the GP values from tetraether lipid membranes cannot be directly compared with the GP values from diester lipid membranes, due to differences in probe disposition. Laurdan’s GP and red-edge excitation shift (REES) values in Sa-MVs and LUVMV decrease with increasing temperature monotonically with no sign for lipid phase transition. Laurdan’s REES values are high (9.3–18.9 nm) in the tetraether lipid membrane systems (i.e., Sa-MVs, LUVMV and LUVPLFE) and low (0.4–5.0 nm) in diester liposomes. The high REES and low GP values suggest that Laurdan in tetraether lipid membranes, especially in the membrane of Sa-MVs, is in a very motionally restricted environment, bound water molecules and the polar moieties in the tetraether lipid headgroups strongly interact with Laurdan’s excited state dipole moment, and “solvent” reorientation around Laurdan’s chromophore in tetraether lipid membranes occurs very slowly compared to Laurdan’s lifetime.
关键词： glycerol dialkyl glycerol tetraether (GDGT),liposomes,intrinsic protein fluorescence,microvesicles,red edge excitation shift (REES),glycerol dialkyl calditol tetraether (GDNT),generalized polarization (GP),thermoacidophilic archaea,membrane probe,Laurdan
更新于2025-09-11 14:15:04
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Self‐assembly of lipid rafts revealed by fluorescence correlation spectroscopy in living breast cancer cells
摘要： In the study, imaging this polarisation Lipids and proteins in the plasma membrane are laterally heterogeneous and formalised as lipid rafts featuring unique biophysical properties. However, self-assembly mechanism of lipid raft cannot be revealed even its physical properties and components were determined in specific physiological two-photon processes. generalised and fluorescence correlation spectroscopy were used to study the fusion of lipid rafts through the membrane phase and the lateral diffusion of lipids in living breast cancer cells. A self-assembly model of lipid rafts and lipid associated with to phase was membrane demonstrate the lipid sorting ability of lipid rafts in the plasma membrane. The results showed that the increased proportion of slow subdiffusion of GM1-binding cholera toxin B-subunit (CT-B) is accompanied with an increased liquid-ordered domain during the β-estradiol-induced fusion of lipid rafts. And slow subdiffusion of CT-B was vanished with the depletion of lipid rafts. Whereas the dialkylindocarbocyanine (DiIC18) diffusion was not specifically regulated by lipid rafts. This study will open up a new insight for uncovering the self-assembly of lipid rafts in specific pathophysiological processes.
关键词： Lipid rafts,Self-assembly,Membrane phase,Fluorescence correlation spectroscopy,Anomalous diffusion
更新于2025-09-11 14:15:04