- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Miniaturized pH Holographic Sensors for the Monitoring of Lactobacillus casei Shirota Growth in a Microfluidic Chip
摘要： Bioreactors have been used both to develop new, and to improve bioprocess yields for, biopharmaceutical products. However, efforts to miniaturize bioreactors, in order to save costs and accelerate process development times, have been limited by the lack of on-site monitoring capabilities available at such scales. In this study, small volume (3nL) non-consumptive holographic sensors were integrated into a glass-PDMS microfluidic chip to monitor via a blue-shift in the resultant holographic replay wavelength, the change in pH during microbial growth of Lactobacillus casei (L. casei) Shirota. Within the optimal growth pH range of L. casei, the accuracy of the miniaturized pH sensors was comparable to that of a conventional pH meter. Conceivably, this approach could be extrapolated to an array of miniaturized holographic sensors sensitive to different analytes, and thereby paving the way for reliable, real-time, non-invasive monitoring of microorganisms in a nanobioreactor.
关键词： microbioreactor,biosensor,pH monitoring,Holographic sensor,bacterial growth,microfluidic chip,nanobioreactor
更新于2025-09-23 15:22:29
-
Microfluidic Platform for Integrated Plasmonic Detection in Laminal Flow
摘要： In this work, we propose a novel approach to design robust microfluidic devices with integrated plasmonic nanoparticles. A strong electric field generated in the gaps between AuBPs – due to the generation of the so-called extrinsic ‘hot-spots’ – was subsequently proved by the volumetric SERS detection of molecules in continuous flow conditions by loading the analyte into the microfluidic channel via a syringe pump. In conclusion, our miniaturized portable microfluidic system aims to detect and identify in real-time with high accuracy analyte molecules in laminal flow, providing thus a groundwork for further complex biosensing applications.
关键词： Gold Bipyramids,Detection in Laminal Flow,LSPR,Microfluidic Chip,SERS
更新于2025-09-23 15:21:01
-
Design and preparation of centrifugal microfluidic chip integrated with SERS detection for rapid diagnostics
摘要： A microfluidic SERS chip integrated with blood separation and in-situ detection was designed and fabricated for the rapid detection of clinical blood samples. Each functional unit in the microfluidic SERS chip consist of separation-decantation cavity based on centrifugal separation principle, mixing channels and SERS detection chamber built with integrated nano-Au on Ag film microstructure. The serum creatinine was selected as a typical sample to demonstrate the capability of microfluidic SERS chip. It was found that the creatinine SERS characteristic peaks at 678 cm-1 can be effectively identified and the detection limit could be as low as 4.42 × 10-3 μmol mL-1 in water. The blood samples were also tested in microfluidic SERS chip. The whole separation and test process could be completed within 2 min, which is a significant improvement in the field of creatinine detection. The whole blood of six cases clinical blood samples were also tested, and the results were consistent with the enzymatic results. The developed microfluidic SERS chip has advantages including reduction of the required quantity of blood sample, reusable and easy to operate. It is expected to provide a new method for rapid diagnostics.
关键词： Surface enhanced Raman spectroscopy (SERS),blood separation,centrifugal microfluidic chip,Ag film@nano-Au substrate,rapid diagnostics
更新于2025-09-23 15:21:01
-
Advanced Design of Microfluidic Chip Based on SPP-LSP Plasmonic Coupling for SERS Detection with High Sensitivity and Reliability
摘要： In this work, we propose the preparation and investigation of advanced microfluidic Surface-enhanced Raman spectroscopy (SERS) chip with the design, allowing high SERS enhancement and analysis reproducibility. Proposed chip implements the creation of periodical metal structure (grating) inside the microfluidic chip and further immobilization of gold multibranched nanoparticles (AuMs) with shaped edges on the grating surface. Such approach allows achieving plasmonic coupling between the surface plasmon polariton wave, excited on the Au grating, and localized surface plasmon, excited on sharped edges of AuMs. As a result, the high enhancement of electric field in the space between AuMs was achieved, which results in the high SERS enhancement factor, confirmed by both, theoretical calculation and experimental measurements with typical SERS analyte – R6G. In particular, vanishingly small concentration of R6G is possible to detect using the proposed plasmonic coupling, which sensitivity significantly exceeds previously reported limits in the case of microfluidic SERS measurements. We also observed the dependency of SERS intensity on the microfluidic flow rate and demonstrated the perfect reliability of the SERS signal, measured in the microfluidic regimes under the constant flow rate.
关键词： SERS,plasmonic coupling,gold multibranched nanoparticles,microfluidic chip,R6G,Surface-enhanced Raman spectroscopy
更新于2025-09-11 14:15:04
-
Quantitative Determination of Urine Glucose: Combination of Laminar Flow in Microfluidic Chip with SERS Probe Technique
摘要： A surface-enhanced Raman scattering(SERS) sensing approach for urine glucose was developed based on the laminar flow technology in a cross-type microfluidic chip with SERS probes, 4-mercaptophenylboronic acid (MPBA) functionalized Ag nanoparticles. MPBA as the glucose receptor can identify and bind up with glucose at a molar ratio of 2:1, which can cause the aggregation of SERS probes at a certain position of the chip channel and further enhance the SERS signal of MPBA significantly. Thus, the quantitative SERS detection of glucose was achieved indirectly. No sample pretreatment and separation were needed in this method since the SERS detection was achieved in the gradient diffusion and molecular recognition processes between urine glucose and SERS probe in the laminar flow, which simplified the sample treatment procedures, saved detection time and made it feasible for clinic applications. This method shows a good linear relationship within human body’s normal physiological range and has high sensitivity and selectivity. The lowest detection concentration can reach 1.0 mg/dL.
关键词： Urine glucose,4-Mercaptophenylboronic acid,Microfluidic chip,Surface-enhanced Raman scattering(SERS)
更新于2025-09-10 09:29:36
-
[IEEE 2018 IEEE 3rd International Conference on Image, Vision and Computing (ICIVC) - Chongqing (2018.6.27-2018.6.29)] 2018 IEEE 3rd International Conference on Image, Vision and Computing (ICIVC) - Low Resolution Cell Image Edge Segmentation Based on Convolutional Neural Network
摘要： An low-resolution cell images captured by a lens-free imaging system is presented in this paper. The resolution of this cell images is impacted by the low and experimental cell segmentation methods to solve the original cell images is not robust and sensitive to noise. So based on the convolutional neural network, an optimized CSnet method is proposed in this paper for automatically segmenting cell. In the proposed method, the produced data set will be sent into the convolutional neural network firstly for training to obtain an optimized convolution neural network segmentation model. And then, the pre-divided images acquired by the lens-free imaging system are loaded into the segmentation model to get the segmentation images. Finally, our proposed method in this paper is tested in a neural network framework built in keras. The experimental results show that the accuracy of our proposed method can reach about 96%. At the same time, it also can implement batch segmentation automatically and make the problem of heavy task for segmentation better.
关键词： convolutional neural network,cell segmentation,Lensfree imaging,microfluidic chip
更新于2025-09-10 09:29:36