- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats
摘要： Purpose The purpose of this study was to evaluate the effectiveness of a photosensitizer hypericin-glucamine, activated by LED amber (34.10 J/cm2) as an adjuvant to scaling and root planing (SRP), on the treatment of experimental periodontal disease (PD) in rats. Methods In a 15-day period, PD was induced in the mandibular molars through ligature placement. The animals (n = 60) were randomly divided into four groups: control (with induction of periodontal disease), scaling and root planing (SRP), antimicrobial photodynamic therapy (aPDT), and SRP + aPDT. At 7, 15, and 30 days after treatments, animals were euthanized. Digital microtomography, histometric, and stereometric analyses were performed to calculate the bone loss of mandibular second molars, and the tissue repair was analyzed histologically. The data were subjected to statistical analysis (α = 5%). Results Histologically, the control group periodontium showed several morphological changes, but an evident gradual reduction in the inflammatory process was observed in the subsequent periods. The SRP, aPDT, and SRP + aPDT groups presented the same, but in less intensity. The stereometric analysis showed a significantly higher proportion of fibroblasts in SRP group (p < 0,001) and SRP + aPDT group (p < 0.0001) compared to that in the control group at 7 days post treatment. Conclusions We conclude that scaling and root planing with or without photodynamic therapy with hypericin-glucamine increased density of the fibroblast and cell density. However, there is no statistically significant difference between SRP and SRP + aPDT.
关键词： Periodontal disease,Hypericin-glucamine,Scaling and root planing
更新于2025-09-23 15:22:29
-
Photoacoustic imaging for monitoring periodontal health: A first human study
摘要： The gold-standard periodontal probe is an aging tool that can detect periodontitis and monitor gingival health but is highly error-prone, does not fully characterize the periodontal pocket, and causes pain. Photoacoustic imaging is a noninvasive technique that can address these limitations. Here, a range of ultrasound frequencies between 16-40 MHz were used to image the periodontium and a contrast medium based on cuttlefish ink was used to label the pockets. A 40 MHz ultrasound frequency could spatially resolve the periodontal anatomy, including tooth, gum, gingival margin, and gingival thickness of tooth numbers 7-10 and 22-27. The photoacoustic-ultrasound measurements were more precise (0.01 mm) than those taken with physical probes by a dental hygienist. Furthermore, the full geometry of the pockets could be visualized with relative standard deviations of 10% (n = 5). This study shows the potential for non-invasive monitoring of periodontal health with photoacoustic-ultrasound imaging in the dental clinic.
关键词： periodontal disease,gingival thickness,periodontal charting,Photoacoustic imaging,melanin nanoparticles,diagnosis,pocket depth
更新于2025-09-23 15:21:01
-
Adjunctive antimicrobial photodynamic therapy using methylene blue/ethanol formulation in experimental periodontitis in diabetic rats: short-term results
摘要： The aim of this study was to evaluate the effect of an MB experimental formulation (ethanol 20%) in aPDT used as an adjuvant to scaling and root planing (SRP) in the periodontal treatment of diabetic rats. Forty male Wistar rats received streptozotocin-intraperitonial injections to induce diabetes. After 14 days, 5 animals were allocated in the non-ligate group (NLG), and 35 animals received ligature at the first right mandibular molar to induce periodontitis. After 7 days, the ligature was removed and the animals were randomized into 4 groups: LG (without treatment, n = 5), SRPG (SRP, n = 10), aPDTW (SRP+aPDT-MB/water, n = 10), and aPDTEt (SRP + aPDT-MB/water/ethanol/carboxymethylcellulose, n = 10). Animals were euthanized after 7 days. Data of bone loss (BL) area, degree of inflammatory cell response, and collagen fibers percentages were statistically analyzed (p < 0.05). Percentage of animals that presented mild and severe inflammatory infiltrate was 10% and 40% for SRPG, 20% and 30% for aPDTW, and 50% and 0% for aPDTEt, respectively. BL area (mm2) was statistically higher in the LG (0.39 ± 0.15) than NLG (0.05 ± 0.02). aPDTEt showed the lowest value of BL (0.08 ± 0.03), followed by aPDTW (0.21 ± 0.15) and SRPG (0.31 ± 0.18). Statistical differences were verified between aPDTEt and SRPG. In relation to the LG, aPDTEt, aPDTW, and SRPG recovered the equivalent 80%, 46%, and 20% of the BL. aPDTEt showed collagen content statistically higher than SRPG and LG, and presented higher mean values than NLG (p > 0.05). Our findings showed aPDTEt presented promising results. aPDT using MB/ethanol can have potential as an adjunctive periodontal treatment in diabetics.
关键词： Alveolar bone loss,Periodontal disease,Root planing,Phototherapy
更新于2025-09-19 17:15:36
-
Comparison between 940 nm Laser Diode and Topical 5 % Potassium Nitrate for Tooth Desensitization. Randomized Controlled Clinical trial
摘要： Purpose: To compare the effectiveness of two dental desensitization therapies, 940nm laser diode and potassium nitrate, in patients with dental hypersensitivity (DH) symptoms. Methods: This randomized clinical trial included 30 patients who initially underwent basic periodontal therapy and presented gingival recessions Class I and II (Miller 1985) with symptoms of DH. Subjects were randomly assigned into two groups. The first group (n = 15) received laser 940 nm application for 90 seconds on the surface of the recession and were given a tube of glycerin to be applied daily in order to avoid generating bias. Patients in the second group (n = 15) were exposed to inactive laser simulating the actual application of the laser, and they were given a desensitizing gel containing 0.5 g of potassium nitrate to be applied twice daily during 14 days. Controls were carried out at 15 minutes, 8 days, 15 days, and 4 weeks. Data obtained were filled out in an Excel table. For comparison between groups the Mann Whitney U and t student tests were used (p=0.05). Results: All patients involve in the study experienced reduction in DH (p<0.01) and differences between the two interventions performed. Conclusions: Laser and nitrate therapies were effective to manage DH after basic periodontal therapy. However, a statistically significant higher sensitivity reduction was observed in laser therapy group.
关键词： diode laser,dentinal sensitivity,periodontal disease,gingival recession,potassium nitrate
更新于2025-09-11 14:15:04