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Sharp pH-responsive mannose prodrug polypeptide nanoparticles encapsulating a photosensitizer for enhanced near infrared imaging-guided photodynamic therapy
摘要： Mannose has been reported as a novel drug to kill cancer cells. The prodrug of mannose will promote its targeted delivery and enrichment at the tumor site and cancer cells. Here, a pH-sensitive polypeptide copolymer with a tertiary amine group has been prepared and a mannose molecule was conjugated to the polymer through the formation of a Schiff base. At the same time, an iodinated boron dipyrromethene (BDPI) photosensitizer with high singlet oxygen generation efficacy and near infrared (NIR) fluorescence was encapsulated by the nanoparticles, which makes it a potential pH-sensitive NIR imaging-guided chemotherapy/PDT agent. In vitro and in vivo studies reveal that in a tumor acidic environment, the protonation of the tertiary amine group destroyed the nanostructure of the nanoparticles, resulting in increased BDPI release. Meanwhile, the bond cleavage of the Schiff base led to the release of conjugated mannose and synergistic inhibition of tumor cell growth with the PDT effect was realized. The combination of these two kinds of tumor suppression effects and photodynamic therapy made this pH-sensitive polypeptide delivery system show great potential for further cancer therapy.
关键词： polypeptide nanoparticles,mannose prodrug,pH-responsive,photodynamic therapy,photosensitizer,near infrared imaging
更新于2025-09-16 10:30:52
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A Randomized, Vehicle-Controlled Phase 3 Study of Aminolevulinic Acid Photodynamic Therapy for the Treatment of Actinic Keratoses on the Upper Extremities
摘要： BACKGROUND Blue-light aminolevulinic acid photodynamic therapy (ALA-PDT) after broad-area application and 3-hour incubation is ef?cacious for actinic keratosis (AK) lesion clearance on upper extremities, with use of occlusive dressing signi?cantly increasing ef?cacy. OBJECTIVE To prove the safety and ef?cacy of ALA-PDT versus vehicle (VEH-PDT) in the spot treatment of multiple AKs on upper extremities. METHODS Aminolevulinic acid or VEH was spot applied only to lesions on one upper extremity 3 hours before blue-light exposure. Treated extremity was covered with occlusive dressing during incubation. Identical treatment was repeated at Week 8 if AK lesions were present in the treated area. RESULTS Thirty-one percent (42/135) of subjects treated with ALA-PDT had complete clearance at Week 12, compared with 13% (17/134) of the subjects treated with VEH-PDT (p = .0001). The mean AK lesion clearance rate for ALA-treated subjects at Weeks 8 and 12 was 53% and 69%, respectively, compared with 26% and 30% for the VEH-treated group (p < .0001, linear mixed model). Safety pro?le observed in this study is consistent with previous studies/reports in the literature, and the therapy was well tolerated overall. CONCLUSION Aminolevulinic acid-PDT spot treatment using a 3-hour occluded incubation was superior to VEH-PDT for AK lesion clearance of the upper extremity.
关键词： actinic keratosis,photodynamic therapy,aminolevulinic acid,upper extremities,blue light
更新于2025-09-16 10:30:52
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Determination of Infrared Laser Energy Dose for Cancer Cells Inactivation as a Candidate of Photodynamic Therapy
摘要： The aim of this research is to find out the effect of GaAlAs diode laser 808 nm exposure on MCF-7 breast cancer cells in vitro with and without the addition of a photosensitizer. Methylene blue (C16H18N3SCl) with concentration of 2 μM is used as the photosensitizer based on the preliminary results of cytotoxicity assay. Energy dose is set in the range of 23,043 to 322,062 J/cm2. Laser exposure with the addition of the photosensitizer generates the highest percentage of cell death of 20.80% at energy dose of 184,344 J/cm2 and cell death begin to decrease at energy dose above this value. This behavior is likely caused by photodegradation and photobleaching effect of the photosensitizer due to the longer exposure time. However, the percentage of cell death without the addition of photosensitizer is increased proportionally to the increase of energy dose and achieved 32,45% cell mortality at 299,559 J/cm2. This study not only shows that the exposure of infrared laser can be used to inactivate cancer cells but also determines its optimum energy dose, makes it a possible candidate for photodynamic therapy in the future.
关键词： MCF-7 cells,photodynamic therapy,photochemical,methylene blue,diode laser,photothermal
更新于2025-09-16 10:30:52
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Antimicrobial Efficacy of Photodynamic Therapy, Diode Laser, and Sodium Hypochlorite and Their Combinations on Endodontic Pathogens
摘要： Background: Sterilization of the root canal system is of prime importance for a successful root canal therapy. Lasers and photodynamic therapy (PDT) have become the latest choice to eradicate microorganisms in the root canal. Objective(s): To evaluate and compare the antimicrobial activity of diode laser, photodynamic therapy, and sodium hypochlorite along with their combinations on endodontic pathogens: Enterococcus faecalis and Streptococcus mutans. Methods: A total of 120 uniradicular teeth were stored in 5.2% NaOCl solution to remove organic residues and the crowns were sectioned at the cemento-enamel junction (CEJ) to obtain root canal length of 15 mm. Canals were shaped using step-back technique up to #40 K-file and were autoclaved. The selected teeth (specimen) were randomly divided into two equal groups with sixty root canals inoculated with E. faecalis (Group E) and remaining sixty teeth with S. mutans (Group S). Further, the groups were subdivided according to the disinfection technique used. Ten teeth from each subgroup were disinfected with a diode laser, photo activated disinfection (PAD), sodium hypochlorite, a combination of sodium hypochlorite and diode laser, a combination of sodium hypochlorite and PAD respectively. Ten teeth in each group served as control without any disinfection. The treated specimens were transferred to test tubes containing 5ml sterile Luria Bertani broth, incubated and the bacterial count, optical density in each root specimen was calculated and compared. The pairwise comparison of colonies across the subgroups was done by the Kruskal-Wallis test and within the subgroups was carried out using the Mann-Whitney U test. The statistical implication was tested at 5% and the study was performed using SPSS 18.0 ver. (SPSS Inc.). Results: A significant reduction (98%) in the E. faecalis count was observed when the NaOCl was used in combination with the diode laser or PAD. PAD along with 3% NaOCl presents the advantage of utilizing a lower wavelength laser beam. Hence, PAD in combination with NaOCl can be an alternative and better option for root canal disinfection for both the endodontic pathogens, E. faecalis and S. mutans.
关键词： E. faecalis,disinfectant,PDT,sodium hypochlorite,PAD,photodynamic therapy,Diode laser,S. mutans
更新于2025-09-16 10:30:52
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[IEEE 2018 IEEE 8th International Conference Nanomaterials: Application & Properties (NAP) - Zatoka, Ukraine (2018.9.9-2018.9.14)] 2018 IEEE 8th International Conference Nanomaterials: Application & Properties (NAP) - Efficient Two-Photon Luminescence for Bioimaging Using Polymer Conjugations of Graphene Quantum Dots Based Materials
摘要： In this study, examination results revealed that conjugated polymers containing nitrogen and sulfur atoms lead to a higher quantum confinement of emissive energy trapped on the surface of material (graphene quantum dot (GQD)-polymers), resulting in a high luminescence quantum yield and impressive two-photon properties. Additionally, the GQD-polymers generated nonreactive oxygen species-dependent oxidative stress on cells. Furthermore, we demonstrated the effective use of two-photon excitation-mediated high two-photon luminescence intensity in an acidic environment enabled GQD-polymers to act as a promising contrast probe. When cancer cells are labeled with specific antibody GQD-polymers conjugates, molecular-specific imaging can be performed deep into a tissue phantom with extremely high signal-to-noise ratios. In situations in which imaging depths are limited by the maximum available power that can be delivered to the three-dimensional (3D) bioimaging plane without causing damage to tissue, GQD-polymers might provide sufficient brightness to extend the maximum depth of imaging. Moreover, we demonstrated that the use of GQD-polymers can expand the capabilities of two-photon imaging to allow noninvasive 3D bioimaging of a variety of new molecular signatures.
关键词： photostability,reactive oxygen species,three-dimensional bioimaging,photodynamic therapy,two-photon excitation,contrast probe,graphene,quantum dot-polymer,two-photon luminescence,two-photon,contrast agent,nitrogen-doped graphene quantum dots
更新于2025-09-16 10:30:52
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Photodynamic Therapy Versus Glucose for the Treatment of Telangiectasia: A Randomised Controlled Study in a Rabbit Ear Model
摘要： This experimental animal study shows that photodynamic therapy (PDT) of telangiectasia signi?cantly reduces the treated vein length and area temperature without causing severe histological damage, such as necrosis or ulceration. These preliminary ?ndings should stimulate further tests using this new approach for the treatment of telangiectasia.
关键词： Hypertonic glucose solution,Photodynamic therapy,Telangiectasia,Rabbit ear model
更新于2025-09-16 10:30:52
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ZnCl2 Enabled Synthesis of Highly Crystalline and Emissive Carbon Dots with Exceptional Capability to Generate O2a??a??
摘要： Highly crystalline and emissive carbon dots (CDs) are ?rstly synthesized via an ionothermal method in organic solvents. ZnCl2 is utilized as the pyrolysis-promoting agent to prepare emissive CDs with tunable wavelengths, directly by carbonization from different O and N precursors under a mild reaction condition. Excellent synthetic yield and photoluminescent quantum yield are obtained. The resulting CDs exhibit excellent capability to photogenerate superoxide radical anion (O2 photooxidative cyclization reactions.
关键词： ZnCl2,photodynamic therapy,carbon dots,photocatalysis,ionothermal synthesis
更新于2025-09-16 10:30:52
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Corea??shell polymeric nanoparticles co-loaded with photosensitizer and organic dye for photodynamic therapy guided by fluorescence imaging in near and short-wave infrared spectral regions
摘要： Background: Biodistribution of photosensitizer (PS) in photodynamic therapy (PDT) can be assessed by fluorescence imaging that visualizes the accumulation of PS in malignant tissue prior to PDT. At the same time, excitation of the PS during an assessment of its biodistribution results in premature photobleaching and can cause toxicity to healthy tissues. Combination of PS with a separate fluorescent moiety, which can be excited apart from PS activation, provides a possibility for fluorescence imaging (FI) guided delivery of PS to cancer site, followed by PDT.
关键词： Polymeric nanoparticles,Poly-N-isopropylacrylamide,Electronic excitation energy transfer,Photodynamic therapy,Short wave infrared fluorescence bioimaging
更新于2025-09-16 10:30:52
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Fractional 1,927?nm Thulium Laser Plus Photodynamic Therapy Compared and Combined for Photodamaged Décolleté Skin: A Side‐by‐Side Randomized Controlled Trial
摘要： Background and Objectives: Décolleté photodamage is a common condition typically treated with light and energy‐based devices. This study investigated the efficacy and safety of a fractional 1,927 nm thulium laser (TL) alone and combined with photodynamic therapy (PDT). Study Design/Materials and Methods: In a 12‐week follow‐up study, participant décolletés were divided into four treatment areas and randomized to receive a single treatment with field‐directed TL, PDT, combination TL‐PDT, or lesion‐directed curettage control. All actinic keratoses (AKs) underwent lesion‐directed curettage before randomization. TL was delivered at 20 mJ/mb, 500 mJ/cm2 fluence, 5 W, and 8 (n = 6 pts.) or 16 (n = 6 pts.) passes. PDT was performed with 16% methyl aminolevulinate (MAL) creme incubated for 3 h, followed by red light‐emitting diode light at 37 J/cm2. Outcome measures included clinical assessment of overall photodamage and specific subcomponents, assisted by optical coherence tomography (OCT) imaging. Results: Twelve women with moderate to severe photodamage on the décolleté and a cumulative total of 184 thin grade I AKs were included. Field‐directed treatments TL and combination TL‐PDT equally improved the overall photodamage, mottled pigmentation, and rhytides compared with lesion‐directed control (P < 0.05). The skin texture improved by TL alone and was further improved by combining TL and PDT (P < 0.05). Median AK complete responses were similar for field‐directed interventions TL‐PDT (100%), TL (90%), PDT (82%), and lesion‐directed curettage control (52%) (P = 0.464). Patients presented with mild local skin responses, slightly more pronounced when combining TL with PDT versus individual treatments (P < 0.05). No scarring or adverse events were observed. Conclusions: The 1,927 nm fractional thulium laser is an effective, tolerable, and safe field‐directed treatment for décolleté photodamage. Provided alone, TL proved to be as effective as combined TL‐PDT for overall photodamage, while a greater improvement in skin texture was achieved using TL and PDT in combination.
关键词： décolleté,optical coherence tomography,skin photodamage,thulium laser,non‐ablative laser,actinic keratosis,skin rejuvenation,photodynamic therapy
更新于2025-09-12 10:27:22
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Evaluations of antibacterial effects of 5-aminolevulinic acids in combination with light emitting diode (LED: 635nm) with different disinfection methods
摘要： This study evaluates the in vitro antibacterial effects of 5-ALA (photoactive dye) in regard to different disinfection methods (2% Chlorhexidine gluconate, 1% Sodium Hypochlorite) on cariogenic bacteria (Streptococcus mutans and Streptococcus sobrinus). After inoculation of dentin specimens with S. mutans and S. sobrinus separately, the reduction in bacterial count was evaluated by the colony counting method. A total of sixty specimens were assigned to six groups (Ten per each group, with five in each group inoculated with S. mutans and the other five inoculated with S.sobrinus); group one: positive control where no treatment was performed, group two: 1% NaOCl was applied, group three: 2% CHX was used, group four: 5-ALA with LED light (635nm) was applied, group five: 5-ALA was used without LED light activation, group six: LED light was used without dye application. There was a significant reduction in the number of S. mutans in the group treated with 5-ALA with LED light, followed by CHX and then NaOCl, whilst minimum bacterial reduction was detected in the groups that were treated with LED light alone and 5-ALA without LED. However, NaOCl exhibited a better effect regarding reduction of S. sobrinus, followed by 5-ALA and LED light and then CHX. All the disinfected groups showed a significant relation with the positive control non-treated group(p<0.05).
关键词： Caries disinfection,5-Aminolevulinic acid,Streptococcus mutans,Photodynamic therapy,Streptococcus sobrinus
更新于2025-09-12 10:27:22