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- 摘要
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- 实验方案
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Selection of a Spectral Index for Detection of Orange Spotting Disease in Oil Palm (Elaeis guineensis Jacq.) Using Red Edge and Neural Network Techniques
摘要： Spectral screening can play an important role in successful detection of viroid-infected oil palm seedlings from nursery stage prior to transplanting into the ?eld. Coconut cadang–cadang viroid (CCCVd) is the main causal agent of orange spotting (OS) disease. OS disease is an emerging disease in Malaysian plantation. In this study, a glasshouse experiment was conducted with ?fteen CCCVd-inoculated and ?ve healthy oil palm seedlings in the growing season of 2015. Spectral screening was performed using a hyperspectral spectroradiometer, Analytic Spectral Device HandHeld 2 (325–1075 nm). The red edge, a steep gradient in re?ectance between red and near-infrared bands (680–780 nm), was used for selection of red edge bands. A maximum point (i.e., 700 nm) and minimum point (i.e., 768 nm) of red edge were selected from healthy and inoculated spectra. Shifts of red edge in?ection point from healthy to inoculated spectra were also studied. Four well-known spectral indices, namely simple ratio, red edge normalized difference vegetation index, two-band enhanced vegetation index 2 (EVI2), and chlorophyll index red edge, were evaluated using selected red edge bands. The multilayer perceptron neural network model was used to establish a nonlinear relationship between selected spectral bands and each spectral index. EVI2 was selected as a best spectral index which resulted in zero errors at the training, testing, and validation datasets. The highest coef?cient of correlation (r = 1) was recorded between spectral bands (input values) and EVI2 (target values).
关键词： Orange spotting,Red edge,EVI2,Spectroradiometer,MLPNN
更新于2025-09-23 15:23:52
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Assessment of red-edge vegetation indices for crop leaf area index estimation
摘要： This study explores the potential of vegetation indices (VIs) for crop leaf area index (LAI) estimation, with a focus on comparing red-edge reflectance based (RE-based) and the visible reflectance based (VIS-based) VIs. Seven VIs were derived from multi-temporal RapidEye images to correlate with LAI of two crop species having contrasting leaf structures and canopy architectures: spring wheat (a monocot) and canola (a dicot) in northern Ontario, Canada. The relationship between LAI and the selected VIs (LAI-VI) was characterized using a semi-empirical model. The Markov Chain Monte Carlo (MCMC) sampling method was used to estimate the model parameters, including the extinction coefficient (KVI) and VI value for dense green canopy (VI∞). Results showed that crop-specific regression models were much closer to a generic regression model using the RE-based VIs than using the VIS-based VIs. Furthermore, the joint posterior probability distribution of the KVI and VI∞ of the RE-based VIs tended to converge for the two crops. This suggests that the RE-based VIs are not as sensitive to canopy structure, e.g., the average leaf angle (ALA), as the VIS-based VIs. This is also demonstrated by the sensitivity analyses using both PROSAIL simulations and field measurements. Hence, the RE-based VIs can be used to develop a more generic LAI estimation algorithm for different crops. Further studies are required to assess the impact of soil reflectance and other factors, such as illumination-target-viewing geometries and atmospheric conditions, on LAI retrieval.
关键词： Sensitivity analysis,Crops,RapidEye,Leaf area index,red-edge,Vegetation index
更新于2025-09-23 15:23:52
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Sulfolobus acidocaldarius Microvesicles Exhibit Unusually Tight Packing Properties as Revealed by Optical Spectroscopy
摘要： In this study, we used optical spectroscopy to characterize the physical properties of microvesicles released from the thermoacidophilic archaeon Sulfolobus acidocaldarius (Sa-MVs). The most abundant proteins in Sa-MVs are the S-layer proteins, which self-assemble on the vesicle surface forming an array of crystalline structures. Lipids in Sa-MVs are exclusively bipolar tetraethers. We found that when excited at 275 nm, intrinsic protein fluorescence of Sa-MVs at 23 °C has an emission maximum at 303 nm (or 296 nm measured at 75 °C), which is unusually low for protein samples containing multiple tryptophans and tyrosines. In the presence of 10–11 mM of the surfactant n-tetradecyl-β-d-maltoside (TDM), Sa-MVs were disintegrated, the emission maximum of intrinsic protein fluorescence was shifted to 312 nm, and the excitation maximum was changed from 288 nm to 280.5 nm, in conjunction with a significant decrease (>2 times) in excitation band sharpness. These data suggest that most of the fluorescent amino acid residues in native Sa-MVs are in a tightly packed protein matrix and that the S-layer proteins may form J-aggregates. The membranes in Sa-MVs, as well as those of unilamellar vesicles (LUVs) made of the polar lipid fraction E (PLFE) tetraether lipids isolated from S. acidocaldarius (LUVPLFE), LUVs reconstituted from the tetraether lipids extracted from Sa-MVs (LUVMV) and LUVs made of the diester lipids, were investigated using the probe 6-dodecanoyl-2-dimethylaminonaphthalene (Laurdan). The generalized polarization (GP) values of Laurdan in tightly packed Sa-MVs, LUVMV, and LUVPLFE were found to be much lower than those obtained from less tightly packed DPPC gel state, which echoes the previous finding that the GP values from tetraether lipid membranes cannot be directly compared with the GP values from diester lipid membranes, due to differences in probe disposition. Laurdan’s GP and red-edge excitation shift (REES) values in Sa-MVs and LUVMV decrease with increasing temperature monotonically with no sign for lipid phase transition. Laurdan’s REES values are high (9.3–18.9 nm) in the tetraether lipid membrane systems (i.e., Sa-MVs, LUVMV and LUVPLFE) and low (0.4–5.0 nm) in diester liposomes. The high REES and low GP values suggest that Laurdan in tetraether lipid membranes, especially in the membrane of Sa-MVs, is in a very motionally restricted environment, bound water molecules and the polar moieties in the tetraether lipid headgroups strongly interact with Laurdan’s excited state dipole moment, and “solvent” reorientation around Laurdan’s chromophore in tetraether lipid membranes occurs very slowly compared to Laurdan’s lifetime.
关键词： glycerol dialkyl glycerol tetraether (GDGT),liposomes,intrinsic protein fluorescence,microvesicles,red edge excitation shift (REES),glycerol dialkyl calditol tetraether (GDNT),generalized polarization (GP),thermoacidophilic archaea,membrane probe,Laurdan
更新于2025-09-11 14:15:04
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[IEEE IGARSS 2018 - 2018 IEEE International Geoscience and Remote Sensing Symposium - Valencia, Spain (2018.7.22-2018.7.27)] IGARSS 2018 - 2018 IEEE International Geoscience and Remote Sensing Symposium - Estimation Model for Dust-Retention Content of Main Green Plants in South China Based on the Red Edge of Reflectance
摘要： Estimating dust-retention content (DRC) on plants leaves is important to the protection and improvement of the atmospheric environment, which can be helpful to the human health and social development. Due to lacking of well-used estimation model, the current studies about dust-retention based on remote sensing are scarce. This paper aims to establish an estimation model for DRC of main green plants based on 56 in situ samples collected in 2014-2016 from Guangzhou, South China. After pre-processing and laboratory analysis, the correlation coefficient between leaf reflectance spectral data and DRC was calculated under MATLAB environment. The results showed that the relationship between red edge (720 nm) of reflectance spectra and DRC is relatively higher. Therefore, a model for estimating DRC based on red edge position was established. It was found that the exponential model showed a high calibration accuracy (DRC: 0.019-1.1 g/m2, R2=0.71, N=40, P-value < 0.001) and had an acceptable validation accuracy (DRC: 0.154-0.978 g/m2, RMSE = 0.157 g/m2, MRE=33.8%, N=16). Although the red edge of vegetation was generally used to monitor the health status of plants, the results of this study indicated that red edge of reflectance can be applied for estimating the DRC on plants leaves as well. Based on the DRC retrieval model, there is potential to monitor and estimate the dynamic changes of dust-retention in a large region from multi-source satellite and unnamed aerial vehicle platform.
关键词： dust-retention content,green plants,red edge,South China,estimation model
更新于2025-09-10 09:29:36
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Evaluating Leaf and Canopy Reflectance of Stressed Rice Plants to Monitor Arsenic Contamination
摘要： Arsenic contamination is a serious problem in rice cultivated soils of many developing countries. Hence, it is critical to monitor and control arsenic uptake in rice plants to avoid adverse effects on human health. This study evaluated the feasibility of using re?ectance spectroscopy to monitor arsenic in rice plants. Four arsenic levels were induced in hydroponically grown rice plants with application of 0, 5, 10 and 20 μmol¨ L′1 sodium arsenate. Re?ectance spectra of upper fully expanded leaves were acquired over visible and infrared (NIR) wavelengths. Additionally, canopy re?ectance for the four arsenic levels was simulated using SAIL (Scattering by Arbitrarily Inclined Leaves) model for various soil moisture conditions and leaf area indices (LAI). Further, sensitivity of various vegetative indices (VIs) to arsenic levels was assessed. Results suggest that plants accumulate high arsenic amounts causing plant stress and changes in re?ectance characteristics. All leaf spectra based VIs related strongly with arsenic with coef?cient of determination (r2) greater than 0.6 while at canopy scale, background re?ectance and LAI confounded with spectral signals of arsenic affecting the VIs’ performance. Among studied VIs, combined index, transformed chlorophyll absorption re?ectance index (TCARI)/optimized soil adjusted vegetation index (OSAVI) exhibited higher sensitivity to arsenic levels and better resistance to soil backgrounds and LAI followed by red edge based VIs (modi?ed chlorophyll absorption re?ectance index (MCARI) and TCARI) suggesting that these VIs could prove to be valuable aids for monitoring arsenic in rice ?elds.
关键词： SAIL model,spectral re?ectance,vegetative indices,arsenic uptake,leaf chlorophyll,red edge,plant stress,soil re?ectance,LAI,rice
更新于2025-09-09 09:28:46
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Rotation and translation dynamics of coumarin 153 in choline chloride-based deep eutectic solvents
摘要： The equilibrium and dynamic solvation responses of coumarin 153 (C153) in a range of deep eutectic solvents (DESs) based on choline chloride with either urea (molar ratio 1:2, ChCl:U), glycerol (1:2, ChCl:G), ethylene glycol (1:2, ChCl:E), or malonic acid (1:1, ChCl:Mal) were investigated using both steady-state and time-resolved ?uorescence emission spectroscopy at room temperature (298 K). From steady-state ?uorescence data, “red-edge effects” were observed in all the DESs studied, attributed to spatial heterogeneity of the DES matrix. Time-resolved Stokes shifts were used to quantify dynamic solvation with the solvation response function in DES found to be a biexponential function of time, which were used to obtain average solvation times ((cid:104)τs(cid:105)) which are generally faster in DES than in ionic liquids of comparable viscosity. Average solvation times showed a partial correlation with viscosity between different DESs. The choline chloride-glycerol DES showed deviation from the viscosity trend observed in the other DES for both dynamic and steady-state results. Rotational reorientation times obtained from dynamic anisotropy (r(t)) measured for these DESs showed a partial correlation with viscosity between different DESs. Determination of the DES rotational coupling with C153 showed more “slip”-like behavior than the previously reported ionic liquids and dipolar solvents.
关键词： solvation dynamics,red-edge effects,deep eutectic solvents,rotational reorientation,coumarin 153
更新于2025-09-09 09:28:46
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A Liquid Crystal Tunable Filter-Based Hyperspectral LiDAR System and Its Application on Vegetation Red Edge Detection
摘要： In this letter, a hyperspectral light detection and ranging (HSL) with 10-nm spectral resolution was designed and tested using a supercontinuum laser source. The major difference between the prototyped HSL and similar instruments was that a liquid crystal tunable ?lter (LCTF) was installed before the avalanche photodiode detector and utilized as a spectroscopic device. The design allowed continuous wavelength selection of the backscattered echoes in the time dimension. Moreover, for general accuracy evaluation of range measurement and spectral measurement, laboratory experiments for vegetation red edge detection were performed using the prototyped HSL to assess its feasibility on agriculture application. Yellow and green leaves from aloe and dracaena plants were measured by the LCTF-HSL for detecting the corresponding “red edge” position. Spectral pro?les measured by an SVC-HR-1024 spectrometer which is designed by SVC company were used as a reference to evaluate the measurements of HSL. The comparison results showed that the red edge positions extracted from the two individual measurements were similar, thus indicating that the LCTF-based high-resolution HSL was effective for this application.
关键词： red edge,liquid crystal tunable ?lter (LCTF),Hyperspectral light detection and ranging (LiDAR) (HSL)
更新于2025-09-09 09:28:46
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Potential of UAV-Based Active Sensing for Monitoring Rice Leaf Nitrogen Status
摘要： Unmanned aerial vehicle (UAV) based active canopy sensors can serve as a promising sensing solution for the estimation of crop nitrogen (N) status with great applicability and ?exibility. This study was endeavored to determine the feasibility of UAV-based active sensing to monitor the leaf N status of rice (Oryza sativa L.) and to examine the transferability of handheld-based predictive models to UAV-based active sensing. In this 3-year multi-locational study, varied N-rates (0–405 kg N ha?1) ?eld experiments were conducted using ?ve rice varieties. Plant samples and sensing data were collected at critical growth stages for growth analysis and monitoring. The portable active canopy sensor RapidSCAN CS-45 with red, red edge, and near infrared wavebands was used in handheld mode and aerial mode on a gimbal under a multi-rotor UAV. The results showed the great potential of UAV-based active sensing for monitoring rice leaf N status. The vegetation index-based regression models were built and evaluated based on Akaike information criterion and independent validation to predict rice leaf dry matter, leaf area index, and leaf N accumulation. Vegetation indices composed of near-infrared and red edge bands (NDRE or RERVI) acquired at a 1.5 m aviation height had a good performance for the practical application. Future studies are needed on the proper operation mode and means for precision N management with this system.
关键词： RapidSCAN,ultra low-level airborne,active canopy sensor,sensing distance evaluation,red edge
更新于2025-09-04 15:30:14
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Adjustment of Sentinel-2 Multi-Spectral Instrument (MSI) Red-Edge Band Reflectance to Nadir BRDF Adjusted Reflectance (NBAR) and Quantification of Red-Edge Band BRDF Effects
摘要： Optical wavelength satellite data have directional re?ectance effects over non-Lambertian surfaces, described by the bidirectional re?ectance distribution function (BRDF). The Sentinel-2 multi-spectral instrument (MSI) acquires data over a 20.6? ?eld of view that have been shown to have non-negligible BRDF effects in the visible, near-infrared, and short wave infrared bands. MSI red-edge BRDF effects have not been investigated. In this study, they are quanti?ed by an examination of 6.6 million (January 2016) and 10.7 million (April 2016) pairs of forward and back scatter re?ectance observations extracted over approximately 20? × 10? of southern Africa. Non-negligible MSI red-edge BRDF effects up to 0.08 (re?ectance units) across the 290 km wide MSI swath are documented. A recently published MODIS BRDF parameter c-factor approach to adjust MSI visible, near-infrared, and short wave infrared re?ectance to nadir BRDF-adjusted re?ectance (NBAR) is adapted for application to the MSI red-edge bands. The red-edge band BRDF parameters needed to implement the algorithm are provided. The parameters are derived by a linear wavelength interpolation of ?xed global MODIS red and NIR BRDF model parameters. The ef?cacy of the interpolation is investigated using POLDER red, red-edge, and NIR BRDF model parameters, and is shown to be appropriate for the c-factor NBAR generation approach. After adjustment to NBAR, red-edge MSI BRDF effects were reduced for the January data (acquired close to the solar principal where BRDF effects are maximal) and the April data (acquired close to the orthogonal plane) for all the MSI red-edge bands.
关键词： Landsat,NBAR,POLDER,bidirectional re?ectance distribution function (BRDF),Africa,Sentinel-2,red-edge,MODIS
更新于2025-09-04 15:30:14