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[Advances in Experimental Medicine and Biology] || Photoresponsive Hydrogels with Photoswitchable Stiffness: Emerging Platforms to Study Temporal Aspects of Mesenchymal Stem Cell Responses to Extracellular Stiffness Regulation
摘要： An extensive number of cell-matrix interaction studies have identi?ed matrix stiffness as a potent regulator of cellular properties and behaviours. Perhaps most notably, matrix stiffness has been demonstrated to regulate mesenchymal stem cell (MSC) phenotype and lineage commitment. Given the therapeutic potential for MSCs in regenerative medicine, signi?cant efforts have been made to understand the molecular mechanisms involved in stiffness regulation. These efforts have predominantly focused on using stiffness-de?ned polyacrylamide (PA) hydrogels to culture cells in 2D and have enabled elucidation of a number of mechano-sensitive signalling pathways. However, despite proving to be a valuable tool, these stiffness-de?ned hydrogels do not re?ect the dynamic nature of living tissues, which are subject to continuous remodelling during processes such as development, ageing, disease and regeneration. Therefore, in order to study temporal aspects of stiffness regulation, researchers have developed and exploited novel hydrogel substrates with in situ tuneable stiffness. In particular, photoresponsive hydrogels with photoswitchable stiffness are emerging as exciting platforms to study MSC responses to extracellular stiffness regulation. This chapter provides an introduction to the use of PA hydrogel substrates, the molecular mechanisms of mechanotransduction currently under investigation and the development of these emerging photoresponsive hydrogel platforms.
关键词： Photoswitchable stiffness,Biophysical regulation,Photoresponsive,Cell-matrix interaction,Regenerative medicine,Polyacrylamide,Mechanotransduction,Stiffness regulation,Mesenchymal stem cells,Cell adhesion,Temporal,Hydrogel substrates
更新于2025-09-23 15:21:01
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A simple, static preservation system for shipping retinal pigment epithelium cell sheets
摘要： The ability to move cells and tissues from bench to bedside is an essential aspect of regenerative medicine. In this study, we propose a simple, static shipping system to deliver tissue-engineered cell sheets. Notably, this system is electronic-device-free and simplified to minimize the number of packing and opening steps involved. Shipping conditions were optimized, and application and verification of the system were performed using human iPS-cells-derived or fetal retinal pigment epithelium (RPE) cell sheets. The temperature of the compartments within the insulated container was stable at various conditions, and filling up the cell vessel with medium effectively prevented turbulence-induced mechanical damage to the RPE cell sheets. Furthermore, no abnormal changes were observed in RPE morphology, transepithelial electrical resistance, or mRNA expression after transit by train and car. Taken together, our simple shipping system has the potential to minimize the costs and human error associated with bench to bedside tissue transfer. This specially designed regenerative tissue shipping system, validated for use in this field, can be used without any special training. This study provides a procedure for easily sharing engineered tissues with the goal of promoting collaboration between laboratories and hospitals and enhancing patient care.
关键词： shipping,induced pluripotent stem cells,product packaging,retinal pigment epithelium,human,regenerative medicine,temperature
更新于2025-09-19 17:15:36
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Biomaterials and Regenerative Medicine in Ophthalmology || An introduction to ophthalmic biomaterials and their role in tissue engineering and regenerative medicine
摘要： The ultimate goal of the research and development of materials (other than drugs) for applications in medicine, which we call biomaterials, has always been to emulate natural materials. Since the natural target for biomaterials, ie, our body’s tissues and organs, is exceedingly complex, it is not surprising that in many instances the laboratory-made materials cannot match in their performance the natural entities they are meant to augment or replace. This is obviously different from the development of materials for industrial applications, which usually perform better than their natural counterparts (if the latter exist), and also evolve relatively fast, unhindered by the biological constraints inherent to living systems. For too long, an acceptable end performance in the short term was the main requirement from a biomaterial, with little attention paid to changing its bulk and/or surface properties through the manipulation of composition and/or structure, in order to maximize the clinical outcome. Over the past six decades or so, however, the progress in bringing the properties and functionality of biomaterials close to those of their biological targets has been remarkable. While the previous statements are valid for the ophthalmic biomaterials too, their development has shown some particular features. The general developments in the field of biomaterials have customarily been gauged through the achievements in the branches of orthopaedic biomaterials and, to a lesser extent, biomaterials for cardiology or dentistry, while the progress of biomaterials for the eye has usually been ignored or seldom presented.
关键词： ophthalmic biomaterials,artificial cornea,poly(methyl methacrylate),contact lenses,hydrogels,cornea,regenerative medicine,tissue engineering
更新于2025-09-10 09:29:36
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[Fundamental Biomedical Technologies] Advanced High-Resolution Tomography in Regenerative Medicine (Three-Dimensional Exploration into the Interactions between Tissues, Cells, and Biomaterials) || In-Line X-Ray Phase Tomography of Bone and Biomaterials for Regenerative Medicine
摘要： The aim of this chapter is to present recent developments in X-ray tomography using in-line phase contrast and their applications to mineralised tissue, whether bone or artificial biomaterials, at micro- and nanoscale. Recently, the main efforts in reconstruction algorithms for in-line X-ray phase contrast imaging have been to push resolution towards the nanoscale and extend the possibilities for quantitative imaging to more general objects. The first is made possible by the use of X-ray optics and the second by the introduction of more advanced priors in the reconstruction. We summarise here these developments and outline recent applications of these techniques, namely, nano-tomography of the ultrastructure of bone and micro-tomography of bone formation in artificial bone grafts as well as in healthy growing mice. While still relatively little used in the field of regenerative medicine, we hope that these examples will stimulate further studies in this field.
关键词： mineralised tissue,regenerative medicine,biomaterials,bone,X-ray tomography,micro-tomography,in-line phase contrast,nano-tomography
更新于2025-09-09 09:28:46
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An NIR-II Fluorescence/Dual Bioluminescence Multiplexed Imaging for In Vivo Visualizing the Location, Survival, and Differentiation of Transplanted Stem Cells
摘要： The in vivo distribution, viability, and differentiation capability of transplanted stem cells are vital for the therapeutic efficacy of stem cell–based therapy. Herein, an NIR-II fluorescence/dual bioluminescence multiplexed imaging method covering the visible and the second near-infrared window from 400 to 1700 nm is successfully developed for in vivo monitoring the location, survival, and osteogenic differentiation of transplanted human mesenchymal stem cells (hMSCs) in a calvarial defect mouse model. The exogenous Ag2S quantum dot–based fluorescence imaging in the second near-infrared window is applied for visualizing the long-term biodistribution of transplanted hMSCs. Endogenous red firefly luciferase (RFLuc)-based bioluminescence imaging (BLI) and the collagen type 1 promoter–driven Gaussia luciferase (GLuc)-based BLI are employed to report the survival and osteogenic differentiation statuses of the transplanted hMSCs. Meanwhile, by integrating the three imaging channels, multiple dynamic biological behaviors of transplanted hMSCs and the promotion effects of immunosuppression and the bone morphogenetic protein 2 on the survival and osteogenic differentiation of transplanted hMSCs are directly observed. The novel multiplexed imaging method can greatly expand the capability for multifunctional analysis of the fates and therapeutic capabilities of the transplanted stem cells, and aid in the improvement of stem cell–based regeneration therapies and their clinical translation.
关键词： near-infrared fluorescence imaging,bioluminescence imaging,transplanted stem cells,stem cell fate,regenerative medicine
更新于2025-09-09 09:28:46
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Imaging approaches in functional assessment of implantable myogenic biomaterials and engineered muscle tissue
摘要： The fields of tissue engineering and regenerative medicine utilize implantable biomaterials and engineered tissues to regenerate damaged cells or replace lost tissues. There are distinct challenges in all facets of this research, but functional assessments and monitoring of such complex environments as muscle tissues present the current strategic priority. Many extant methods for addressing these questions result in the destruction or alteration of tissues or cell populations under investigation. Modern advances in non-invasive imaging modalities present opportunities to rethink some of the anachronistic methods, however, their standard employment may not be optimal when considering advancements in myology. New image analysis protocols and/or combinations of established modalities need to be addressed. This review focuses on efficacies and limitations of available imaging modalities to the functional assessment of implantable myogenic biomaterials and engineered muscle tissues.
关键词： Tissue Engineering,Imaging,Regenerative Medicine,Implantable myogenic biomaterials,Engineered muscles
更新于2025-09-09 09:28:46