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- 2019
- Root Mean Square Error (RMSE)
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- Grid
- Photovoltaic
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Cholesterol Functionalization of Gold Nanoparticles Enhances Photo-Activation of Neural Activity
摘要： Gold nanoparticles (AuNPs) attached to the extracellular leaflet of the plasma membrane of neurons can enable the generation of action potentials (APs) in response to brief pulses of light. Recently described techniques to stably bind AuNP bioconjugates directly to membrane proteins (ion channels) in neurons enable robust AP generation mediated by the photoexcited conjugate. However, a strategy that binds the AuNP to the plasma membrane in a non-protein-specific manner could represent a simple, single-step means of establishing light-responsiveness in multiple types of excitable neurons contained in the same tissue. Based on the ability of cholesterol to insert into the plasma membrane, here we test whether AuNP functionalization with linear dihydrolipoic acid-poly(ethylene) glycol (DHLA-PEG) chains that are distally terminated with cholesterol (AuNP-PEG-Chol) can enable light-induced AP generation in neurons. Dorsal root ganglion (DRG) neurons of rat were labelled with 20 nm diameter spherical AuNP-PEG-Chol conjugates wherein ~30% of the surface ligands (DHLA-PEG-COOH) were conjugated to PEG-Chol. Voltage recordings under current-clamp conditions showed that DRG neurons labeled in this manner exhibited a capacity for AP generation in response to microsecond and millisecond pulses of 532 nm light, a property attributable to the close tethering of AuNP-PEG-Chol conjugates to the plasma membrane facilitated by the cholesterol moiety. Light-induced AP and subthreshold depolarizing responses of the DRG neurons were similar to those previously described for AuNP conjugates targeted to channel proteins using large, multicomponent immunoconjugates. This likely reflected the AuNP-PEG-Chol’s ability, upon plasmonic light absorption and resultant slight and rapid heating of the plasma membrane, to induce a concomitant transmembrane depolarizing capacitive current. Notably, AuNP-PEG-Chol delivered to DRG neurons by inclusion in the buffer contained in the recording pipette/electrode enabled similar light-responsiveness, consistent with the activity of AuNP-PEG-Chol bound to the inner (cytofacial) leaflet of the plasma membrane. Our results demonstrate the ability of AuNP-PEG-Chol conjugates to confer timely stable and direct responsiveness to light in neurons. Further, this strategy represents a general approach for establishing excitable cell photosensitivity that could be of substantial advantage for exploring a given tissue’s suitability for AuNP-mediated photo-control of neural activity.
关键词： nanoparticle functionalization,cholesterol,action potential,neural photo-activation,optocapacitance,gold nanoparticles,photosensitivity,dorsal root ganglion cell
更新于2025-09-23 15:23:52
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[IEEE IGARSS 2018 - 2018 IEEE International Geoscience and Remote Sensing Symposium - Valencia (2018.7.22-2018.7.27)] IGARSS 2018 - 2018 IEEE International Geoscience and Remote Sensing Symposium - Validation of Wave and Wind Product of the New Phase Saral Using Buoys Data
摘要： Since July 4th 2016, the SARAL/Altika has been moved in a new orbit, and it has continuously provided wave height measurements for more than 1 year. Before using these data, the measurements need to be validated. Based on the in-situ buoys from the National Data Buoy Center (NDBC), the SARAL Ka-band significant wave height (SWH) and Sea Surface Wind (SSW) measurements were validated and have been corrected using a linear regression with in-situ measurements. Compared with NDBC data, the validation results of SARAL show a RMS (Root Mean Square) of 0.28 m for SARAL SWH measurements and RMS of 1.30m/s for SARAL SSW speed indicating capability of AltiKa providing SWH and wind speed products with reliable accuracy. Therefore the accuracy of SARAL SWH products is higher than that of Jason-1/2 SWH data, and does not require any correction.
关键词： Sea Surface Wind,SARAL/AltiKa,Validation,Root Mean Square,Significant Wave Height
更新于2025-09-23 15:22:29
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DWARF4 accumulation in root tips is enhanced via blue light perception by cryptochromes
摘要： Brassinosteroid (BR) signalling is known to be coordinated with light signalling in aboveground tissue. Many studies focusing on the shade avoidance response in aboveground tissue or hypocotyl elongation in darkness have revealed the contribution of the BR signalling pathway to these processes. We previously analysed the expression of DWARF 4 (DWF4), a key BR biosynthesis enzyme, and revealed that light perception in aboveground tissues triggered DWF4 accumulation in root tips. To determine the required wavelength of light and photoreceptors responsible for this regulation, we studied DWF4-GUS marker plants grown in several monochromatic light conditions. We revealed that monochromatic blue LED light could induce DWF4 accumulation in primary root tips and root growth as much as white light, while monochromatic red LED could not. Consistent with this, a cryptochrome1/2 double mutant showed retarded root growth under white light while a phytochromeA/B double mutant did not. Taken together, our data strongly indicated that blue light signalling was important for DWF4 accumulation in root tips and root growth. Furthermore, DWF4 accumulation patterns in primary root tips were not altered by auxin or sugar treatment. Therefore, we hypothesize that blue light signalling from the shoot tissue is different from auxin and sugar signalling.
关键词： light signalling,brassinosteroid,DWF4,phytochromes,cryptochromes,hypocotyl elongation,photoreceptor,root growth,BR biosynthesis
更新于2025-09-23 15:22:29
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[IEEE 2018 37th Chinese Control Conference (CCC) - Wuhan (2018.7.25-2018.7.27)] 2018 37th Chinese Control Conference (CCC) - On-line Detection and Analysis of Alloy Steel Elements Based on the LIBS Technology and Random Forest Regression
摘要： The Laser Induced Breakdown Spectroscopy (LIBS) technology can be used to detect the elements in the alloy steel in real time. Quantitative analysis method of the traditional LIBS technology mainly has the calibration method and calibration free method, but there are two shortcomings: low prediction accuracy and over fitting. Random Forest Regression (RFR) algorithm can be used for classification and regression, can effectively avoid 'overfitting' phenomenon. Therefore, in this paper, we combine the random forest regression algorithm with laser induced breakdown spectroscopy applied to the detection of the concentration of alloy steel elements in the metallurgy industry. At the same time, compared with partial least squares method based on the LIBS, the results show that the random forest algorithm combined with the LIBS technology has the higher prediction accuracy, lower root mean square error and better robustness.
关键词： PLS,root mean square error,quantitative analysis,LIBS,RFR
更新于2025-09-23 15:22:29
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Photodegradation of 2-Chlorophenol in TiO2/UV System: Phytotoxicity Assessment of Treated Solutions Against Seedling Growth of Turnip and Tomato
摘要： The photodegradation and detoxification of 2-chlorophenol (2-CP) in TiO2 suspensions were studied using monochromatic UV-lamp. The effect of pH on the photodegradation of 2-CP was investigated. Removal of 98% of initial 2-CP was achieved within 210 under optimum concentration of 2-CP (60 mg/L) and TiO2 (80 mg/L). The apparent first-order rate constant kapp of 2-CP is estimated to be 0.0043 min–1 at pH 4, while a 3-fold increase in kapp was observed at pH 9. Phytotoxicity effects of treated 2-CP on seed germination, root elongation and plant growth of tomato (Lycopersicon esculentum) and turnip (Brassica rapa) seeds were also carried out with the aim of water reuse and environment protection. No statistically differences have been also found to exist for seed germination of tomato and turnip between deionized water (control) and phototreated 2-CP solution, indicating a significant removal of the overall toxicity. In the 10th day of germination, the average root elongation increases at 210 min of photocatalytic degradation yielding 7.1 ± 0.9 and 5.6 ± 0.66 cm for turnip and tomato seeds, respectively. Turnip plants irrigated with the phototreated 2-CP solution grow in a similar manner as the plant irrigated with the tap water. The phototreated 2-CP can be used in the irrigation of agriculture garden but only after further treatment.
关键词： root elongation,seed germination,TiO2,2-chlorophenol
更新于2025-09-23 15:22:29
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THROUGH THE LOOKING GLASS: Real-Time Imaging in Brachypodium Roots and Osmotic Stress Analysis
摘要： To elucidate dynamic developmental processes in plants, live tissues and organs must be visualised frequently and for extended periods. The development of roots is studied at a cellular resolution not only to comprehend the basic processes fundamental to maintenance and pattern formation but also study stress tolerance adaptation in plants. Despite technological advancements, maintaining continuous access to samples and simultaneously preserving their morphological structures and physiological conditions without causing damage presents hindrances in the measurement, visualisation and analyses of growing organs including plant roots. We propose a preliminary system which integrates the optical real-time visualisation through light microscopy with a liquid culture which enables us to image at the tissue and cellular level horizontally growing Brachypodium roots every few minutes and up to 24 h. We describe a simple setup which can be used to track the growth of the root as it grows including the root tip growth and osmotic stress dynamics. We demonstrate the system’s capability to scale down the PEG-mediated osmotic stress analysis and collected data on gene expression under osmotic stress.
关键词： root,real-time imaging,osmotic stress,Casparian bands,Brachypodium,PDMS,neutral red,PEG-6000
更新于2025-09-23 15:22:29
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Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats
摘要： Purpose The purpose of this study was to evaluate the effectiveness of a photosensitizer hypericin-glucamine, activated by LED amber (34.10 J/cm2) as an adjuvant to scaling and root planing (SRP), on the treatment of experimental periodontal disease (PD) in rats. Methods In a 15-day period, PD was induced in the mandibular molars through ligature placement. The animals (n = 60) were randomly divided into four groups: control (with induction of periodontal disease), scaling and root planing (SRP), antimicrobial photodynamic therapy (aPDT), and SRP + aPDT. At 7, 15, and 30 days after treatments, animals were euthanized. Digital microtomography, histometric, and stereometric analyses were performed to calculate the bone loss of mandibular second molars, and the tissue repair was analyzed histologically. The data were subjected to statistical analysis (α = 5%). Results Histologically, the control group periodontium showed several morphological changes, but an evident gradual reduction in the inflammatory process was observed in the subsequent periods. The SRP, aPDT, and SRP + aPDT groups presented the same, but in less intensity. The stereometric analysis showed a significantly higher proportion of fibroblasts in SRP group (p < 0,001) and SRP + aPDT group (p < 0.0001) compared to that in the control group at 7 days post treatment. Conclusions We conclude that scaling and root planing with or without photodynamic therapy with hypericin-glucamine increased density of the fibroblast and cell density. However, there is no statistically significant difference between SRP and SRP + aPDT.
关键词： Periodontal disease,Hypericin-glucamine,Scaling and root planing
更新于2025-09-23 15:22:29
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[IEEE 2019 24th Microoptics Conference (MOC) - Toyama, Japan (2019.11.17-2019.11.20)] 2019 24th Microoptics Conference (MOC) - Patterned emission of organic light emitting diodes with laser irradiation
摘要： Self-healing networks aim to detect cells with service degradation, identify the fault cause of their problem, and execute compensation and repair actions. The development of this type of automatic system presents several challenges to be confronted. The first challenge is the scarce number of historically reported faults, which greatly complicates the evaluation of novel self-healing techniques. For this reason, in this paper, a system model to simulate faults in Long-Term Evolution (LTE) networks, along with their most significant key performance indicators, is proposed. Second, the expert knowledge required to build a self-healing system is usually not documented. Therefore, in this paper, a methodology to extract this information from a collection of reported cases is proposed. Finally, following the proposed methodology, an automatic fuzzy-logic-based system for fault identification in LTE networks is designed. Evaluation results show that the fuzzy system provides fault identification with a high success rate.
关键词： Long-Term Evolution (LTE),Diagnosis,fuzzy logic,fault identification,troubleshooting,root cause analysis,self-healing,fault management
更新于2025-09-23 15:21:01
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On the root mean square error (RMSE) calculation for parameter estimation of photovoltaic models: A novel exact analytical solution based on Lambert W function
摘要： In the literature, one can find a lot of methods and techniques employed to estimate single diode solar photovoltaic (PV) cell parameters. The efficiency of these methods is usually tested by calculating the Root Mean Square Error (RMSE) between the measured and estimated values of the solar PV cell output current. In this work, first, the values of RMSE calculated using 69 different methods published in many journal papers for the well-known RTC France solar PV cell are presented and discussed. Second, a novel exact analytical solution for RMSE calculation based on the Lambert W function is proposed. The results obtained show that the RMSE values were not calculated correctly in most of the methods presented in the literature since the exact expression of the calculated cell output current was not used. Third, the precision of calculation of the methods used for analytical solving of Lambert W equation is presented and discussed. Fourth, the applicability of the proposed solution methodology in accordance with current-voltage characteristics measured in the laboratory for solar modules of Clean Energy Trainer Setup is checked. Identification of its unknown parameters is presented using three optimization techniques. Further, the proposed solution methodology is proven for Solarex MSX–60 PV module, and the most promising 5-parameter single diode parameters are estimated based on minimization of the precise RMSE values calculated. Finally, this work aimed to develop a good base for proper investigation and implementation of optimization algorithms to solve the parameter estimation problem of 5-parameter single diode PV equivalent circuits.
关键词： Root mean square error,5-parameter single diode model,PV parameter estimation,Optimization,RTC France solar cell,Lambert W function
更新于2025-09-23 15:21:01
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[IEEE 2019 IEEE 16th India Council International Conference (INDICON) - Rajkot, India (2019.12.13-2019.12.15)] 2019 IEEE 16th India Council International Conference (INDICON) - Performance Comparison Between Bipolar and Unipolar Switching Scheme for a Single-Phase Inverter Based Stand-alone Photovoltaic System
摘要： Self-organizing network (SON) mechanisms reduce operational expenditure in cellular networks while enhancing the offered quality of service. Within a SON, self-healing aims to autonomously solve problems in the radio access network and to minimize their impact on the user. Self-healing comprises automatic fault detection, root cause analysis, fault compensation, and recovery. This paper presents a root cause analysis system based on fuzzy logic. A genetic algorithm is proposed for learning the rule base. The proposed method is adapted to the way of reasoning of troubleshooting experts, which ease knowledge acquisition and system output interpretation. Results show that the obtained results are comparable or even better than those obtained when the troubleshooting experts define the rules, with the clear benefit of not requiring the experts to define the system. In addition, the system is robust, since fine tuning of its parameters is not mandatory.
关键词： genetic algorithms,self-organizing networks (SONs),Fuzzy systems,troubleshooting,root cause analysis,self-healing,supervised learning
更新于2025-09-23 15:21:01