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[IEEE 2018 International Symposium on Micro-NanoMechatronics and Human Science (MHS) - Nagoya, Japan (2018.12.9-2018.12.12)] 2018 International Symposium on Micro-NanoMechatronics and Human Science (MHS) - Uniform Transfection: Shock Wave Generation in Laser Ablation and Microcontact Printing
摘要： With the increasing need of regenerative medicines, cell therapy, and biopharmaceutical drug, it is necessary to develop a method for massively parallel delivery and uniform transfection. This research explores the possibility of using μCP (using cell adhesive and cell blocking agents) for printing massive number of cells on a substrate and using optoporation methods (nano-second pulsed radiation) to perforate cell membranes. With combining these two methods, a stand-alone micro-device capable of performing localized intracellular delivery will be developed. In this paper, we confirmed the generation of shock wave upon optoporation, tried intra-cellular delivery using Calcein, develop the stamp for micro-contact printing and performed the printing.
关键词： optoporation,laser ablation,uniform transfection,shock wave generation,microcontact printing
更新于2025-09-19 17:13:59
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[IEEE 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Munich, Germany (2019.6.23-2019.6.27)] 2019 Conference on Lasers and Electro-Optics Europe & European Quantum Electronics Conference (CLEO/Europe-EQEC) - Anisotropic Dispersive Shock Waves in Highly Nonlinear M-Cresol/Nylon
摘要： M-Cresol/Nylon is a chemical solution, which is made up of an organic solvent (m-cresol) and a synthetic polymeric solute (nylon). Recent experiments showed that this solution exhibits an isotropic giant self-defocusing nonlinearity, tunable by varying the nylon concentration [1]. When it is enlightened by a continuous-wave laser beam, light absorption induces heat gain, which reduces the refractive index: the material experiences a nonlinear thermo-optical effect, made nonlocal by the heat diffusion. Since the temperature changes with the beam intensity, this nonlinearity follows a nonlocal Kerr model, expressed by the nonlinear Schroedinger equation (NLSE) [2]. In particular, V. Smith et al. measured the m-cresol/nylon nonlinear Kerr coef?cient n2 and found that, if for pure m-cresol it is ?9 × 10?8cm2/W , for a nylon mass concentration of 3.5% it is ?1.6 × 10?5cm2/W , a magnitude higher than most of the other thermal nonlinear materials reported in literature. In optics, many phenomena are ruled by NLSE, such as soliton propagation and dispersive shock wave (DSW) generation [1,2]. We report experimental evidence of two-dimensional optical DSWs with an anisotropic zero-singularity (Fig. 1). Fixing z as the longitudinal and x, y as the transverse directions, we start with a beam that has a Gaussian pro?le along y but a Gaussian ?rst derivative along x, thus the intensity is null for x = 0. This speci?c initial condition causes a new phenomenon, reported here for the ?rst time: the shock develops undular bores on the beam external borders (as already known in literature [2]), but around the singularity it presents an abrupt intensity discontinuity. We theoretically analyze the wave breaking along all the propagation. By a hydrodynamic model, we study the beam before the shock point: the WKB approach and the characteristic method let us predict the wave breaking both in phase and in intensity [2]. In order to model the beam propagation beyond the shock point, we use mathematical tools of time asymmetric quantum mechanics and uncover the mechanism how such an abrupt intensity discontinuity is generated [3]. We numerically simulate these results and ?nd remarkable agreement both with experiments and with theoretical predictions. Our outcome not only con?rms previous studies on the giant nonlinear response of m-cresol/nylon, but also discloses fundamental insights on propagation of DSWs with a singular initial intensity pro?le.
关键词： Dispersive shock wave generation,Anisotropic Dispersive Shock Waves,Highly Nonlinear M-Cresol/Nylon,Nonlinear Schroedinger equation,Thermo-optical effect
更新于2025-09-16 10:30:52