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Simple Lithography-Free Single Cell Micropatterning using Laser-Cut Stencils
摘要： Micropatterning techniques have been widely used in cell biology to study effects of controlling cell shape and size on cell fate determination at single cell resolution. Current state-of-the-art single cell micropatterning techniques involve soft lithography and micro-contact printing, which is a powerful technology, but requires trained engineering skills and certain facility support in microfabrication. These limitations require a more accessible technique. Here, we describe a simple alternative lithography-free method: stencil-based single cell patterning. We provide step-by-step procedures including stencil design, polyacrylamide hydrogel fabrication, stencil-based protein incorporation, and cell plating and culture. This simple method can be used to pattern an array of as many as 2,000 cells. We demonstrate the patterning of cardiomyocytes derived from single human induced pluripotent stem cells (hiPSC) with distinct cell shapes, from a 1:1 square to a 7:1 adult cardiomyocyte-like rectangle. This stencil-based single cell patterning is lithography-free, technically robust, convenient, inexpensive, and most importantly accessible to those with a limited bioengineering background.
关键词： iPSC-CM,Developmental Biology,hydrogel,lithography-free,cell morphology,stencil,single cell micropatterning
更新于2025-09-23 15:21:01
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[IEEE 2019 International Symposium on Electromagnetic Compatibility - EMC EUROPE - Barcelona, Spain (2019.9.2-2019.9.6)] 2019 International Symposium on Electromagnetic Compatibility - EMC EUROPE - (a??Oops! Had the silly thing in reversea??)a??Optical injection attacks in through LED status indicators
摘要： Color breakup is a lethal issue to conventional field-sequential-color LCDs. Several methods have been proposed to solve this problem. However, these solutions are either more than 180 Hz—the currently used LC mode is not sufficiently fast for this field rate—or they degrade the image quality while using a field rate of 120 Hz. To overcome these limitations, a 120 Hz Stencil Local Primary Desaturation, Stencil-LPD, is presented in this work. The 120 Hz Stencil-LPD method provides reduced but sufficient saturated backlight color to precisely present an image with less color distortion or color breakup. After optimizing a number of backlight segments, the experimental results demonstrate that the image fidelity is significantly improved and color breakup is greatly suppressed compared to the existing methods.
关键词： stencil,local-primary-desaturation,field-sequential-color,LPD,FSC,color breakup
更新于2025-09-23 15:21:01
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Transparent, flexible MAPbI3 perovskite microwire array passivated with ultra-hydrophobic supramolecular assembly for stable and high-performance photodetectors
摘要： The emergence of organic-inorganic hybrid perovskites (OHPs) has revolutionised the potential performance of optoelectronic devices, most perovskites are opaque and hence incompatible with transparent optoelectronics, and sensitive to environmental degradation. Here, a single-step fabrication of ultra-long MAPbI3 perovskite microwire array over a large-area using stencil lithography based on a sequential vacuum sublimation. The environmental stability of MAPbI3 is empowered a newly designed and synthesized transparent supramolecular self-assembly, based on a mixture of two tripodal L-Phe-C11H23/C7F15 molecules, which showed a contact angle of 105° and served as ultra-hydrophobic passivation layer for more than 45 days in ambient atmosphere. The MAPbI3 microwire array passivated with supramolecular self-assembly demonstrate for the first time both excellent transparency of ~89% at 550 nm and remarkable photoresponse with photo-switching ratio of ~104, responsivity of 789 A/W, detectivity of 1014 Jones, linear dynamic range of ~ 122 dB, and rise time of 432 μs. Furthermore, the photodetector fabricated on flexible PET substrate demonstrated robust mechanical flexibility even beyond 1200 bending cycles. Therefore, the scalable stencil lithography and supramolecular passivation approaches have the potential to deliver next-generation transparent, flexible, and stable optoelectronics.
关键词： transparency,stencil lithography,photodetectors,MAPbI3,supramolecular self-assembly,flexibility,organic-inorganic hybrid perovskites,environmental stability
更新于2025-09-23 15:21:01