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Influence of osmolytes on the stability of thylakoid‐based dye‐sensitized solar cells
摘要： In recent years, there has been considerable interest in incorporating naturally occurring components of the photosynthetic apparatus into man‐made solar cells, because of the high quantum efficiency of photosynthetic reaction centers. One hurdle to overcome regarding the use of native membranes in these devices is their limited lifespans. In this study, we used stabilizers to increase the long‐term viability of biomolecules in vitro, thereby alleviating this challenge. In this regard, it is known that osmolytes, such as glycine betaine (GB) and sucrose, preserve photosynthetic activity in isolated photosystems. Upon investigation of the thermal protection properties of GB and sucrose in thylakoid‐based dye‐sensitized solar cells, we report that the addition of GB and sucrose to the thylakoid photosensitizer maintains nonzero photocurrent in the thylakoid‐based solar cell upon heating to 50°C. At 50°C, the GB‐containing cell displayed about a fourfold increase in photocurrent than the control cell, in which the photocurrent was decreased to nearly zero. The addition of 0.5M and 1M sucrose has respectively caused nearly 40% and 70% increases in photoinduced electron transfer activity over the control at 35°С. Similarly, though to a lesser extent, 1M GB caused an approximate 40% increase in electron transfer activity as well. Moving forward, this approach will be extended to alternative membrane protein isolation strategies, allowing for an accurate comparison with traditional detergent‐isolated complexes, with the ultimate goal of developing a cost‐effective and sustainable solar cell.
关键词： solar cell,stabilization,thylakoid membrane,sucrose,glycine betaine
更新于2025-09-19 17:13:59
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Response of Synechocystis sp. PCC 6803 to UV radiations by alteration of polyamines associated with thylakoid membrane proteins
摘要： The responses of Synechocystis sp. PCC 6803 exposed to UVA, UVB and UVC for at least 3 h were investigated with the emphasis on the changes of polyamines (PAs) levels in whole cells, thylakoid membrane fraction, and thylakoid membrane-associated proteins fraction. All UV radiations caused a slight decrease on cell growth but a drastic reduction of photosynthetic efficiency of Synechocystis cells. UV radiations, especially UVB and UVC, severely decreased the levels of PAs associated with thylakoid membrane proteins. The decreased PAs levels as affected by UV radiation correlated well with the decrease of photosynthetic efficiency, suggesting the role of PAs for the maintenance of photosynthetic activity of Synechocystis. PAs, especially spermidine (Spd) and putrescine (Put), were found abundantly in the thylakoid membrane fraction, and these PAs were associated mainly with the PSI trimer complex. Importantly, the exposure of Synechocystis cells to all UV radiations for 3 h resulted in the increase of Spd associated with the PSII monomer and dimer complex, suggesting its protective role against UV radiations despite the overall decrease of PAs.
关键词： Polyamine association,Thylakoid membrane protein complexes,Stress response,UV radiations,Synechocystis sp. PCC 6803
更新于2025-09-04 15:30:14