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Fabrication of conductive fibrous scaffold for photoreceptor differentiation of mesenchymal stem cell
摘要： Conductive nanofibrous scaffolds with that can conduct electrical current have a great potential in neural tissue engineering. The purpose of this study was to survey effects of electrical stimulation and polycaprolactone/polypyrrole/multiwall carbon nanotube (PCL/PPY/MWCNTs) fibrous scaffold on photoreceptor differentiation of trabecular meshwork mesenchymal stem cells (TM‐MSCs). PCL/PPY/MWCNTs scaffold was made by electrospinning method. TM‐MSCs were seeded on PCL/PPY/MWCNTs scaffold and stimulated with a potential of 115 V/m. Scanning electron microscopy, transmission electron microscopy, and FT‐IR were used to evaluate the fabricated scaffold. Immunofluorescence and quantitative real‐time polymerase chain reaction were used to examine differentiated cells. Scanning electron microscopy, transmitting electron microscopy, and FT‐IR confirmed the creation of the composite structure of fibers. RT‐qPCR analysis showed that the expression of rhodopsin and peripherin genes in electrically stimulated cells were significantly higher (5.7‐ and 6.23‐fold, respectively; p ≤ 0.05) than those with no electrical stimulation. Collectively, it seems that the combination of PCL/PPY/MWCNTs scaffold, as a suitable conductive scaffold, and electrical stimulation could be an effective approach in the differentiation of stem cells in retinal tissue engineering.
关键词： electrical conductive,trabecular meshwork mesenchymal stem cells,photoreceptor‐like cells,nanostructure
更新于2025-11-14 15:18:02
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Quantification of Focal Outflow Enhancement Using Differential Canalograms
摘要： PURPOSE. To quantify regional changes of conventional out?ow caused by ab interno trabeculectomy (AIT). METHODS. Gonioscopic, plasma-mediated AIT was established in enucleated pig eyes. We developed a program to automatically quantify out?ow changes (R, package eye-canalogram, github.com) using a ?uorescent tracer reperfusion technique. Trabecular meshwork (TM) ablation was demonstrated with ?uorescent spheres in six eyes before formal out?ow quanti?cation with two-dye reperfusion canalograms in six additional eyes. Eyes were perfused with a central, intracameral needle at 15 mm Hg. Canalograms and histology were correlated for each eye. RESULTS. The pig eye provided a model with high similarity to AIT in human patients. Histology indicated ablation of TM and unroo?ng of most Schlemm’s canal segments. Spheres highlighted additional circumferential and radial out?ow beyond the immediate area of ablation. Differential canalograms showed that AIT caused an increase of out?ow of 17 6 5-fold inferonasally, 14 6 3-fold superonasally, and also an increase in the opposite quadrants with a 2 6 1-fold increase superotemporally, and 3 6 3 inferotemporally. Perilimbal speci?c ?ow image analysis showed an accelerated nasal ?lling with an additional perilimbal ?ow direction into adjacent quadrants. CONCLUSIONS. A quantitative, differential canalography technique was developed that allows us to quantify supraphysiological out?ow enhancement by AIT.
关键词： canalogram,glaucoma,trabecular meshwork,segmental out?ow,porcine eyes
更新于2025-09-04 15:30:14