Setting Up Surface-Enhanced Raman Scattering Database for Artificial Intelligence-Based Label-Free Discrimination of Tumor Suppressor Genes

摘要： The quality of input data in deep learning is tightly associated with the ultimate performance of machine learner. Taking advantages of unique merits of surface-enhanced Raman scattering (SERS) methodology in the collection and construction of database (e.g., abundant intrinsic fingerprint information, noninvasive data acquisition process, strong anti-interfering ability, etc.), herein we set up SERS-based database of deoxyribonucleic acid (DNA), suitable for artificial intelligence (AI)-based sensing applications. The database is collected and analyzed by silver nanoparticles (Ag NPs)-decorated silicon wafer (Ag NPs@Si) SERS chip, followed by training with a deep neural network (DNN). As proof-of-concept applications, three kinds of representative tumor suppressor genes, i.e., p16, p21 and p53 fragments, are readily discriminated in label-free manners. Prominent and reproducible SERS spectra of these DNA molecules are collected and employed as input data for DNN learning and training, which enables selective discrimination of DNA target(s). The accuracy rate for the recognition of specific DNA target reaches 90.28%.