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White-emitting protein-metal nanocluster phosphors for highly performing bio-hybrid light-emitting diodes
摘要： This work presents a simple in-situ synthesis and stabilization of fluorescent gold nanoclusters (AuNCs) with different sizes using engineered protein scaffolds in water. The protein-AuNC hybrids show a dual emission (450 and 700 nm) with a record photoluminescence quantum yield of 20%. These features impelled us to apply them to bio-hybrid light-emitting diodes (Bio-HLEDs) as color down-converting filters or bio-phosphors. Efficient white emission (x/y CIE color coordinates of 0.31/0.29) and stabilities of >800 h were achieved. This represents a two orders of magnitude enhancement compared to the prior art. Besides the outstanding performance, the protein scaffold also infers a unique anisotropic emission character that is considered as a proof-of-concept of high interest for single-point lighting and display.
关键词： bio-phosphors,protein design,Light-emitting diodes,repeat proteins,bio-hybrid materials,metal nanocluster
更新于2025-09-19 17:13:59
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Detection of Various Biomarkers and Enzymes via Nanocluster-Based Fluorescence Turn-On Sensing Platform
摘要： The profiling of disease-related biomarkers is an essential procedure for the accurate diagnosis and intervention of metabolic disorders. Therefore, the development of ultrasensitive and highly selective fluorogenic biosensors for diverse biomarkers is extremely desirable. There is still a considerable challenge to prepare nanocluster-based fluorescence turn-on probes capable of recognizing multiple biomolecules. We herein provide a novel nanocluster-based chemical information processing system (CIPS) for the programmable detecting various metabolites and enzymes. This CIPS consists of the biocatalytic reactions between substrates and their respective oxidases to generate H2O2, which was then employed to synthesize bright fluorescent silver nanoclusters (Ag NCs). Utilizing this system, we are able to accurately probe a series of substrates/ corresponding oxidases with high sensitivity and specificity, including glucose/ glucose oxidase, uric acid/ uric acid oxidase, sarcosine/ sarcosine oxidase, choline/ choline oxidase, xanthine/ xanthine oxidase, and lactic acid/ lactic acid oxidase. Furthermore, this metabolite profiling CIPS can integrate with binary logic operations, which create an intelligently tool for the high throughput screening of various diseases in vitro (e.g., diabetes, gout, prostate cancer, cardiovascular disease, and lactic acidosis).
关键词： nanocluster-based fluorescence turn-on sensing platform,silver nanoclusters,chemical information processing system,biomarkers,enzymes
更新于2025-09-09 09:28:46
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Ether-soluble Cu53 Nanoclusters as an Effective Precursor of High-quality CuI Films for Optoelectronic Applications
摘要： An effective strategy is developed to synthesize high-nuclearity Cu clusters, [Cu53(RCOO)10(C≡CtBu)20Cl2H18]+ (Cu53), which is the largest Cu(I)/Cu(0) cluster reported to date. Cu powder and Ph2SiH2 are employed as the reducing agents in the synthesis. As revealed by single-crystal diffraction, Cu53 is arranged as a four-concentric-shell Cu3@Cu10Cl2@Cu20@Cu20 structure, possessing an atomic arrangement of concentric M12 icosahedral and M20 dodecahedral shells which popularly occurs in Au/Ag nanoclusters. Surprisingly, Cu53 can be dissolved in diethyl ether and spin coated to form uniform nanoclusters film on organo-lead halide perovskite. The cluster film can subsequently be converted into high-quality CuI film via in-situ iodination at room temperature. The as-fabricated CuI film proves to be an excellent hole-transport layer for fabricating highly stable CuI-based perovskite solar cells (PSCs) with 14.3% of efficiency.
关键词： CuI film,Cu nanocluster,Alkynyl,Perovskite solar cells,Superatoms
更新于2025-09-09 09:28:46
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Hybridization induced fluorescence enhanced DNA-Ag nanocluster/aptamer probe for detection of prostate-specific antigen
摘要： In this work, a label-free Ag nanocluster (AgNC)-based fluorescent probe is proposed to detect tumor marker, prostate-specific antigen (PSA). In the experiments, DNA sequences containing segments complemented to different parts of PSA aptamer were used to synthesize DNA-Ag nanoclusters (DNA-AgNC). Some of the obtained specific DNA-AgNC exhibited significant fluorescence increase after hybridization with PSA aptamer. Based on this, a simple DNA-AgNC/aptamer hybridization probe was fabricated for PSA detection using fluorescence quenching, because competitively specific binding between PSA and its aptamer inhibited the fluorescence enhancement effect of PSA aptamer on DNA-AgNC. The sequence of template DNA, pH and salt concentration of binding buffer, and the concentration of aptamer were optimized. Under optimum conditions, the concentration of PSA within the range of 2–150 ng mL?1 with the detection limit of 1.14 ng mL?1 was detected (3σ; n = 7). This approach was also successfully applied to determine PSA in spiked serum samples. As is well known, this was the first report to realize PSA detection using fluorescent AgNC-based probe. This work would provide reference for construction of AgNC-based probes for detecting other proteins.
关键词： DNA-AgNC fluorescent probe,Ag nanocluster,Prostate-specific antigen,Aptamer
更新于2025-09-04 15:30:14
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Unexpected Observation of Heavy Monomeric Motifs in a Basket-like Au <sub/>26</sub> Ag <sub/>22</sub> Nanocluster
摘要： Herein, a new basket-like Au26Ag22(TBBT)30 nanocluster (where TBBT = 4-tert-butylthiophenol) was synthesized by a coreduction method. Also, the precise structure was characterized by single-crystal X-ray diffraction and displays a shell-by-shell construction with a Au12@AuAg19 kernel protected by a Au13S26@Ag3S4 surface motif. Interestingly, a handle-like Ag3S4 motif was observed that composes the outermost shell. Except for this Ag3S4 motif, the other motifs are all monomeric “?S?Au?S?” motifs, which are rarely reported in thiolate-capped metal (Au or Ag) nanoclusters with small size (n < 60). This work will provide new insight into the growth pattern of thiolate-capped metal nanoclusters.
关键词： thiolate-capped,Au26Ag22,monomeric motifs,nanocluster,shell-by-shell construction
更新于2025-09-04 15:30:14
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Atomic structure of a fluorescent Ag <sub/>8</sub> cluster templated by a multi-stranded DNA scaffold
摘要： Multinuclear silver clusters encapsulated by DNA exhibit size-tunable emission spectra and rich photophysics, but their atomic organization is poorly understood. Herein, we describe the structure of one such hybrid chromophore, a green-emitting Ag8 cluster arranged in a Big Dipper shape bound to the oligonucleotide A2C4. Three 3’ cytosine metallo-base pairs stabilize a parallel A-form-like duplex with a 5’ adenine-rich pocket, which binds a metallic, trapezoidal-shaped Ag5 moiety via Ag-N bonds to endo- and exo-cyclic nitrogens of cytosine and adenine. The unique DNA configuration, constrained coordination environment, and templated Ag8 cluster arrangement highlight the reciprocity between the silvers and DNA in adopting this structure. These first atomic details of a DNA-encapsulated Ag cluster fluorophore illuminate many aspects of biological assembly, nanoscience, and medical imaging.
关键词： crystallography,photophysics,silver,crystals,DNA,materials,nanocluster,nanomaterials,emissive cluster
更新于2025-09-04 15:30:14
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High Visible Response of Vertical TiO2 Nanoclusters-CdSe Linked by Cysteine
摘要： Vertically oriented single-crystal TiO2 nanoclusters in tetramethylammonium hydroxide solution were synthesized hydrothermally directly on Ti substrate. N-Type semiconducting CdSe nanoparticles were then introduced deep coating TiO2 nanocrystalss via a two-step procedure using cystein as a surface coupling agent, followed by a subsequent electrodeposition process from selenosulfite to form orderly interconnecting heterojunctions. A key result achievement is that although the TiO2 nanocrystalss are less than 1 μm long, under the help of the bifunctional 2-amino-3-sulfhydrylpropanoic acid molecule linking the acceptor and donor materials, the virtue of strong visible absorption of single crystal TiO2 nanoclusters were fully developed in addition of ultraviolet, so that the heterojunction electrode exhibits excellent photoresponse in photoelectrochemical cells to visible lights, whose contribution is rather significant compared with that of ultraviolet lights. Vertical nanoclusters have advantages of leading electrons directly into the metal Ti substrate while maintaining the large surface area towards the electrolyte. These highly ordered nanohybrids are expected to have potential applications in photochemical solar cells with high efficiency.
关键词： TiO2 nanocluster,Visible lights,Sensitization,CdSe,Vertically oriented
更新于2025-09-04 15:30:14