研究目的
The development of ultrasensitive and highly selective fluorogenic biosensors for diverse biomarkers to accurately diagnose and intervene metabolic disorders.
研究成果
The nanocluster-based fluorescence turn-on CIPS was successfully established for the programmable detection of various crucial biomarkers/corresponding oxidases. The PL intensity of CIPS shows a linear relationship to the reactive biomolecular amounts. This metabolite profiling has been integrated to binary logic gates, providing a new strategy for future screening of metabolic diseases or medical diagnostics.
研究不足
The technical and application constraints of the experiments, as well as potential areas for optimization, are not explicitly mentioned in the provided text.
1：Experimental Design and Method Selection:
The CIPS consists of biocatalytic reactions between substrates and their respective oxidases to generate H2O2, which is then used to synthesize fluorescent silver nanoclusters (Ag NCs).
2：Sample Selection and Data Sources:
Various substrates and oxidases were used, including glucose/glucose oxidase, uric acid/uric acid oxidase, sarcosine/sarcosine oxidase, choline/choline oxidase, xanthine/xanthine oxidase, and lactic acid/lactic acid oxidase.
3：List of Experimental Equipment and Materials:
Ferrous sulfate heptahydrate, hydrogen peroxide, acetic acid, sodium acetate trihydrate, sodium citrate, citric acid, silver nitrate, uric acid, sarcosine, D-(+)-Glucose, L-histidine, L-serine, L-arginine, L-tryptophan, L-aspartic acid, glycine, L-lysine, terephthalic acid, choline hydroxide solution, xanthine, glucose oxidase, distilled methacrylic acid, sarcosine oxidase, choline oxidase, lactic acid oxidase, xanthine oxidase, acetylcholine esterase, β-galactosidase, lactic acid, artificial urine.
4：Experimental Procedures and Operational Workflow:
The preparation of Ag NCs involved mixing substrates with oxidases to generate H2O2, which then reacted with ferrous ions to generate hydroxyl radicals, initiating the polymerization of MAA to PMAA. Ag NCs were synthesized using PMAA as a stabilizer.
5：Data Analysis Methods:
UV-vis absorption data, photoluminescence experiments, TEM images, zeta potential, XPS data, FT-IR spectroscopy.
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Ferrous sulfate heptahydrate
Beijing Chemical Works
Used in the generation of hydroxyl radicals for the polymerization of MAA to PMAA.
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Hydrogen peroxide
Beijing Chemical Works
Reacted with ferrous ions to generate hydroxyl radicals for the polymerization of MAA to PMAA.
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Silver nitrate
Beijing Chemical Works
Used in the synthesis of fluorescent silver nanoclusters (Ag NCs).
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Glucose oxidase
Aldrich
Catalyzed the oxidation of glucose to release H2O2.
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Sarcosine oxidase
Yuanye Biological Technology Co., Ltd
Catalyzed the oxidation of sarcosine to release H2O2.
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Choline oxidase
Yuanye Biological Technology Co., Ltd
Catalyzed the oxidation of choline to release H2O2.
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Lactic acid oxidase
Yuanye Biological Technology Co., Ltd
Catalyzed the oxidation of lactic acid to release H2O2.
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Xanthine oxidase
Yuanye Biological Technology Co., Ltd
Catalyzed the oxidation of xanthine to release H2O2.
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Acetylcholine esterase
Yuanye Biological Technology Co., Ltd
Used in the OR logic gate operation for the detection of acetylcholine.
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β-galactosidase
Yuanye Biological Technology Co., Ltd
Used in the OR logic gate operation for the detection of lactose.
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