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An ultrasensitive homogeneous aptasensor for carcinoembryonic antigen based on upconversion fluorescence resonance energy transfer
摘要： Carcinoembryonic antigen (CEA) has been recognized as one of the most important tumor markers. Herein, we reported an ultrasensitive homogeneous aptasensor based on fluorescence resonance energy transfer (FRET) between upconversion nanoparticles (UCNPs) and graphene oxide (GO) for CEA detection. The CEA aptamer modified UCNPs can bind to the surface of GO through π-π stacking interaction, resulting in fluorescence quenching due to the energy transfer from UCNPs to GO. After the introduction of CEA, the CEA aptamer preferentially combined with CEA to form three-dimensional structure which made UCNPs-aptamer dissociate from the GO, blocking the energy transfer process. The fluorescence of UCNPs was accordingly restored in a CEA concentration-dependent manner both aqueous solution and human serum samples. The aptasensor could monitor CEA level directly in human serum and the results were strongly correlated with commercial chemiluminescence kits. The excellent detection performance suggested promising prospect of the aptasensor in practical application.
关键词： Upconversion Nanoparticles,Graphene Oxide,Fluorescence Resonance Energy Transfer,CEA,Aptasensor
更新于2025-09-23 15:21:01
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Highly selective and label-free Love-mode surface acoustic wave biosensor for carcinoembryonic antigen detection using a self-assembled monolayer bioreceptor
摘要： A love-mode surface acoustic wave (SAW) biosensor based on ST-cut quartz was developed for highly selective and label-free detection of carcinoembryonic antigen (CEA). The delay line area of an interdigital transducer (IDT) based SAW device was coated with gold and then chemically modified through thioglycolic acid–EDC/NHS reaction mechanism. A self-assembled monolayer of anti-CEA was further immobilized on the bioreceptors through the coupling layer. The biosensing capability of the SAW device was evaluated using solutions of CEA with various concentrations and limit of detection was obtained at 0.31 ng/ml of CEA, which is better than the results reported by the literatures available for CEA detection using SAW device. The real-time detection capability of the biosensor was evaluated using clinical serum samples and selectivity was evaluated using mixed solutions of CEA with other common tumor marking proteins. Long-term stability of the biosensor was also evaluated over a period of 30 days and the immunoassay response has shown only 8% decrease in performance within the whole period. The binding of CEA onto the bioreceptor was evaluated through Langmuir and Freundlich sorption isotherm kinetic studies as well.
关键词： SAW,Piezoelectricity,Biosensing,CEA,Label free,SAM
更新于2025-09-23 15:19:57
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Perylenetetracarboxylic Dianhydride and Aniline Assembled Supramolecular Nanomaterial with Multi-color Electrochemiluminescence for Highly Sensitive Label-free Immunoassay
摘要： Most electrochemiluminescence (ECL) studies were focused on the single emission of luminophore, which was severely limited the development of multi-color ECL fundamental theory and applications. Herein, we prepared a multi-color ECL supramolecular nanomaterial self-assembled by 3,4,9,10-perylenetetracarboxylic dianhydride (PTCDA) and aniline (An) through hydrogen bonding. This PTCDA-An supramolecular nanomaterial simultaneously produced multi-color emission peaked at 486, 692 and 760 nm with K2S2O8 as coreactant. And the multi-emissions were assigned to excitated PTCDA monomer (486 nm), H-dimer (692 nm) and J-dimer (760 nm). The simultaneously increased dual-color ECL intensity significantly enhanced the total ECL intensity of PTCDA-An. And this high efficient ECL nanomaterial was further used as ECL platform to construct label-free immunosensor for tumor markers carcinoembryonic antigen (CEA) detection. The total ECL intensity of immunosensor exhibited a sensitive decrease due to the simultaneously decreased ECL of multi-emissions. And this immunosensor exhibited wide linear range from 1 pg mL-1 to 10 μg mL-1 with low detection limit of 0.23 pg mL-1. Multi-color ECL from the same luminophore PTCDA in this work also provided a new perspective for multi-color ECL biomaterial’s design.
关键词： supramolecular nanomaterial,multi-color ECL,electrochemiluminescence,aniline,PTCDA,CEA detection,label-free immunosensor
更新于2025-09-19 17:13:59
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Gold/WS2 nanocomposites fabricated by in-situ ultrasonication and assembling for photoelectrochemical immunosensing of carcinoembryonic antigen
摘要： Tungsten disulfide (WS2) nanosheets were obtained by exfoliating WS2 bulk crystals in N-methylpyrrolidone by ultrasonication. Gold nanoparticles (GNPs) were synthesized by in-situ ultrasonication of sodium citrate and HAuCl4 while fabricating the WS2 nanosheets. In this way, the GNPs were self-assembled on WS2 nanosheets to form a GNPs/WS2 nanocomposite through interaction between sulfur and gold atoms. The photoelectrochemical response of WS2 nanosheets is significantly enhanced after integration of the GNPs. The GNPs/WS2 nanocomposite was coated onto a glassy carbon electrode (GCE) to construct a sensing interface which then was modified with an antibody against the carcinoembryonic antigen (CEA) to obtain a photoelectrochemical immunosensor for CEA. Under optimized conditions, the decline in relative photocurrent is linearly related to the logarithm of the CEA concentration in the range from 0.001 to 40 ng mL?1. The detection limit is 0.5 pg mL?1 (at S/N = 3). The assay is sensitive, selective, stable and reproducible. It was applied to the determination of CEA in clinical serum samples.
关键词： Gold nanoparticles,CEA,Photoelectrochemistry,Nanosheets,Transition metal dichalcogenides,Immunosensor
更新于2025-09-10 09:29:36