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Cell viability assessments of green synthesized water-soluble AgInS <sub/>2</sub> /ZnS core/shell quantum dots against different cancer cell lines
摘要： Chalcopyrite quantum dots (QDs) have emerged as a safe alternative to cadmium-based QDs for bio-applications. However, the research on AgInS2 chalcopyrite QDs has not been widely explored in terms of their toxicity. Herein, we report a synthesis of biocompatible AgInS2/ZnS QDs via a greener approach. The emission intensity of the as-synthesized AgInS2 core QDs was enhanced 2-fold after the ZnS shell growth. X-ray diffraction revealed the tetragonal crystal structure of QDs, and high-resolution transmission electron microscope images show that the QDs are spherical in shape and crystalline in nature. Cell viability assays conducted on different cell lines, such as HeLa, A549, and BHK-21 cells, indicated that AgInS2/ZnS QDs are least toxic at a QD concentration range of 100 lg/mL. The ?uorescent microscope analysis of A549 cells incubated with AgInS2/ZnS QDs shows that the QDs were accumulated in the cell membranes. The as-synthesized AgInS2/ZnS QDs are less toxic and eco-friendly, and can be used for biolabeling.
关键词： cell viability,AgInS2/ZnS,biolabeling,Chalcopyrite quantum dots,biocompatibility
更新于2025-11-19 16:46:39
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[OSA Optical Fiber Communication Conference - San Francisco, California (2014..-..)] Optical Fiber Communication Conference - Biorthogonal Modulation in 8 Dimensions Experimentally Implemented as 2PPM-PS-QPSK
摘要： The influence of ambient air species especially humidity is an ever-present challenge for atmospheric pressure plasma jet applications. Especially, where the plasma-induced effects are extremely sensitive to humidity, such as in the field of plasma medicine, an understanding of the influence of ambient species diffusion on plasma chemistry and on reactive component composition is crucial. In this paper, we investigate the influence of ambient humidity versus feed gas humidity on the production of reactive components by atmospheric pressure plasma jets. By the use of a shielding gas curtain, we control the surrounding atmosphere around the active effluent region of the investigated argon RF-plasma jet the ambient gas. By quantum cascade laser absorption spectroscopy and by Fourier transformed infrared (IR) absorption spectroscopy, the effect of diffusing surrounding molecular species on the chemistry of the long-living reactive oxygen species is investigated. Mechanisms of H2O2 and O3 production are studied. In this paper, we have quantified the influence that ambient species, namely, water molecules, have on the reactive species’ generation in the gas phase. It is shown that the effect of ambient humidity is important for the long-living species production, feed gas humidity, however, has the much stronger effect. Finally, with the focus of applications in plasma medicine, the cell viability of human skin cells (HaCaT keratinocytes) as a function of feed gas and ambient gas humidity is compared.
关键词： Ambient humidity,plasma medicine,feed gas humidity,atmospheric pressure plasma jet,cell viability,plasma chemistry
更新于2025-09-23 15:19:57
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[IEEE 2019 IEEE International Conference on Power, Intelligent Computing and Systems (ICPICS) - Shenyang, China (2019.7.12-2019.7.14)] 2019 IEEE International Conference on Power, Intelligent Computing and Systems (ICPICS) - Infrared and Visible Image Fusion Based on CLAHE and Sparse Representation
摘要： The influence of ambient air species especially humidity is an ever-present challenge for atmospheric pressure plasma jet applications. Especially, where the plasma-induced effects are extremely sensitive to humidity, such as in the field of plasma medicine, an understanding of the influence of ambient species diffusion on plasma chemistry and on reactive component composition is crucial. In this paper, we investigate the influence of ambient humidity versus feed gas humidity on the production of reactive components by atmospheric pressure plasma jets. By the use of a shielding gas curtain, we control the surrounding atmosphere around the active effluent region of the investigated argon RF-plasma jet the ambient gas. By quantum cascade laser absorption spectroscopy and by Fourier transformed infrared (IR) absorption spectroscopy, the effect of diffusing surrounding molecular species on the chemistry of the long-living reactive oxygen species is investigated. Mechanisms of H2O2 and O3 production are studied. In this paper, we have quantified the influence that ambient species, namely, water molecules, have on the reactive species’ generation in the gas phase. It is shown that the effect of ambient humidity is important for the long-living species production, feed gas humidity, however, has the much stronger effect. Finally, with the focus of applications in plasma medicine, the cell viability of human skin cells (HaCaT keratinocytes) as a function of feed gas and ambient gas humidity is compared.
关键词： plasma chemistry,atmospheric pressure plasma jet,feed gas humidity,Ambient humidity,plasma medicine,cell viability
更新于2025-09-23 15:19:57
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PC 12 Pheochromocytoma Cell Response to Super High Frequency Terahertz Radiation from Synchrotron Source
摘要： High frequency (HF) electromagnetic ?elds (EMFs) have been widely used in many wireless communication devices, yet within the terahertz (THz) range, their effects on biological systems are poorly understood. In this study, electromagnetic radiation in the range of 0.3–19.5 × 1012 Hz, generated using a synchrotron light source, was used to investigate the response of PC 12 neuron-like pheochromocytoma cells to THz irradiation. The PC 12 cells remained viable and physiologically healthy, as con?rmed by a panel of biological assays; however, exposure to THz radiation for 10 min at 25.2 ± 0.4 ?C was suf?cient to induce a temporary increase in their cell membrane permeability. High-resolution transmission electron microscopy (TEM) con?rmed cell membrane permeabilization via visualisation of the translocation of silica nanospheres (d = 23.5 ± 0.2 nm) and their clusters (d = 63 nm) into the PC 12 cells. Analysis of scanning electron microscopy (SEM) micrographs revealed the formation of atypically large (up to 1 μm) blebs on the surface of PC 12 cells when exposed to THz radiation. Long-term analysis showed no substantial differences in metabolic activity between the PC 12 cells exposed to THz radiation and untreated cells; however, a higher population of the THz-treated PC 12 cells responded to the nerve growth factor (NGF) by extending longer neurites (up to 0–20 μm) compared to the untreated PC12 cells (up to 20 μm). These ?ndings present implications for the development of nanoparticle-mediated drug delivery and gene therapy strategies since THz irradiation can promote nanoparticle uptake by cells without causing apoptosis, necrosis or physiological damage, as well as provide a deeper fundamental insight into the biological effects of environmental exposure of cells to electromagnetic radiation of super high frequencies.
关键词： super high frequency,electromagnetic radiation,cell viability,PC 12 neuronal cells,terahertz exposure
更新于2025-09-19 17:15:36
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A Cell Viability Evaluation Method Based on Respiratory Thermodynamic Feature Detected by Microscopic Infrared Thermal Imaging Sensor
摘要： Cell viability is an important indicator while screening drugs. An inaccurate evaluation of cell viability can cause large errors in anti-tumor dose experiments, and this becomes very unfavorable for cancer treatment. In this paper, there is a new cell viability evaluation method based on respiratory thermodynamic feature that includes the respiratory intensity, proliferation rate, and heat released by cells. These three parameters can be directly measured by a microscopic infrared thermal imaging sensor, which is fast and non-invasive and does not require consumables. The three parameters were simultaneously measured based on a micro-infrared thermal imaging sensor and fitted to the mathematical model. Finally, the method was verified by comparing it with traditional counting method and comparing the amount of omethoate with traditional counting method. The results indicated that omethoate is 12.36% lower than in the traditional counting method with the same level of complete cell inactivation. Therefore, this method is more accurate than the conventional cell viability assessment method, and the dosage is more precise when the uniform effect is achieved, which provides a basis for precise doses in tumor treatment and can reduce side effects in the human body. This method has a significant effect on the manufacture of cell activity detecting sensors.
关键词： Micro-infrared thermal imaging,Cell respiration,Yeast,Cell viability evaluation,Metabolic ability
更新于2025-09-19 17:13:59
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[IEEE 2019 IEEE 8th International Conference on Advanced Optoelectronics and Lasers (CAOL) - Sozopol, Bulgaria (2019.9.6-2019.9.8)] 2019 IEEE 8th International Conference on Advanced Optoelectronics and Lasers (CAOL) - The Stabilization of the Bolometera??s Heat-Exchange Coefficient with the Environment
摘要： The influence of ambient air species especially humidity is an ever-present challenge for atmospheric pressure plasma jet applications. Especially, where the plasma-induced effects are extremely sensitive to humidity, such as in the field of plasma medicine, an understanding of the influence of ambient species diffusion on plasma chemistry and on reactive component composition is crucial. In this paper, we investigate the influence of ambient humidity versus feed gas humidity on the production of reactive components by atmospheric pressure plasma jets. By the use of a shielding gas curtain, we control the surrounding atmosphere around the active effluent region of the investigated argon RF-plasma jet the ambient gas. By quantum cascade laser absorption spectroscopy and by Fourier transformed infrared (IR) absorption spectroscopy, the effect of diffusing surrounding molecular species on the chemistry of the long-living reactive oxygen species is investigated. Mechanisms of H2O2 and O3 production are studied. In this paper, we have quantified the influence that ambient species, namely, water molecules, have on the reactive species’ generation in the gas phase. It is shown that the effect of ambient humidity is important for the long-living species production, feed gas humidity, however, has the much stronger effect. Finally, with the focus of applications in plasma medicine, the cell viability of human skin cells (HaCaT keratinocytes) as a function of feed gas and ambient gas humidity is compared.
关键词： plasma medicine,cell viability,feed gas humidity,plasma chemistry,Ambient humidity,atmospheric pressure plasma jet
更新于2025-09-19 17:13:59
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APPLICATION OF LASER-INDUCED BREAKDOWN CAVITATION BUBBLES FOR CELL LYSIS IN VITRO
摘要： Objective: Understanding the basic mechanism of the cavitation bubble action on living cells as a crucial step of development and application of sophisticated methods based on controlled cavitation in cell behaviour manipulation. Optimisation of parameters in order to expand cell lysis region created by a single bubble. Methods: The cavitation bubbles are generated by the laser-induced breakdown method. The impact of controlled cavitation bubble on the biological system is synchronously monitored under a microscope and recorded. Visualization of the cavitation bubble course is monitored by a high-speed camera. The impact of technology on the healthy confluent cell layer is verified. Evaluation of the cavitation bubbles′ effect on cells in real time and by subsequent analysis of the cell lysis region and impact of the cavitation bubble on cell viability is carried out by optical visualization and life/dead fluorescence staining. Results: Cavitation bubble induced in distance of 1.5 mm from the cell surface overcomes properties of sessile bubble and enables to create cell lysis region over 1000 μm in diameter due to transient shear stress produced by liquid displaced by the bubble expansion. Conclusion: Cell lysis region is strongly dependent on the spot laser energy (SLE) and the bubble induction distance from cells. This knowledge is crucial for application in chemical free cell lysis in vitro, wound induction for experimental purposes and cell layers patterning in desired scale.
关键词： Laser-induced breakdown,Cavitation bubble,Cell viability,Cell lysis
更新于2025-09-12 10:27:22
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Facile, large scale synthesis of water soluble AgInSe2/ZnSe quantum dots and its cell viability assessment on different cell lines
摘要： I-III-VI chalcopyrite ternary quantum dots have emerged as a good alternative over the conventional II-VI and IV-VI chalcogenide binary QDs that usually consist of heavy metals such as Cd and Pb which has limited their bioapplications. Among the chalcopyrite QDs, AgInSe2 QDs has been the least developed due to the imbalanced cation reactivity, unwanted impurities, broad size distribution and resultant large particle sizes. In addition, the cell viability of these QDs still needs to be investigated on different cell lines both normal and cancerous ones. Herein, large-scale synthesis of water-soluble thioglycolic acid (TGA) capped and gelatin-stabilized AgInSe2 (AISe) core and AgInSe2/ZnSe (AISe/ZnSe) core/shell QDs in the absence of an inert atmosphere and their cell viability against different cell lines are reported. The optical and structural characteristics of the as-synthesized QDs were investigated by UV–visible (vis) absorption, photoluminescence (PL) and Fourier-transmission infrared (FTIR) spectroscopies, dynamic light scattering (DLS), X-ray diffraction (XRD), and high-resolution transmission electron microscope (HRTEM) techniques. Growth of ZnSe shell on the core AISe resulted in the blue shifting of the emission maximum position with the increased PL intensity. The QDs are small and spherical in shape with an average particle diameter of 2.8 nm and 3.2 nm for AISe and AISe/ZnSe QDs respectively. The in vitro cell viability assay revealed that the as-synthesized AISe/ZnSe QDs are not toxic towards cancerous (HeLa -cervical cancer and A549-lung cancer) and normal (BHK21 –Kidney) cell lines.
关键词： I-III-VI quantum dots,Cell viability,AgInSe2/ZnSe,Biocompatibility
更新于2025-09-12 10:27:22
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Influence of size and surface capping on photoluminescence and cytotoxicity of gold nanoparticles
摘要： Hydrophilic and homogeneous sub-10 nm blue light-emitting gold nanoparticles (NPs) functionalized with different capping agents have been prepared by simple chemical routes. Structure, average, size, and surface characteristics of these NPs have been widely studied, and the stability of colloidal NP solutions at different pH values has been evaluated. Au NPs show blue PL emission, particularly in the GSH capped NPs, in which the thiol-metal core transference transitions considerably enhance the fluorescent emission. The influence of capping agent and NP size on cytotoxicity and on the fluorescent emission are analyzed and discussed in order to obtain Au NPs with suitable features for biomedical applications. Cytotoxicity of different types of gold NPs has been determined using NPs at high concentrations in both tumor cell lines and primary cells. All NPs used show high biocompatibility with low cytotoxicity even at high concentration, while Au-GSH NPs decrease viability and proliferation of both a tumor cell line and primary lymphocytes.
关键词： Semiconfocal microscopy,Photoluminescence,Capping,Cell viability,GSH,Gold nanoparticles,Agent,Proliferation,Cytotoxicity
更新于2025-09-10 09:29:36