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Silicon photomultipliers applied to the fluorescence detection of biomarkers
摘要： The silicon photomultiplier (SiPM) has advantages that allow it to detect low levels of light intensity in medical, chemical and biological applications; it can also build integrated, portable microfluidic systems. This paper presents a SIPM application designed for the detection of fluorescence of biomarkers. In order to minimise the volume of the tested sample, the measurements were conducted in a microfluidic system. A data acquisition system for the SiPM has been designed consisting of a dedicated application specific integrated circuit (ASIC) required to amplify and shape signals from the photodetector. The application has been designed with the aim of transforming it into a portable device. The detection method chosen in the research is based on flow cytometry and single molecule detection [1,2]. Although only a single photodetector was used, the presented applications can be adapted to multi-detector measurements. Initially, the application measured the fluorescence of fluorophores. Afterwards, it was used in the detection of Anti-NPR1 to confirm the effectiveness of the method. The application is not dedicated to a single antigen. It can be applied for the detection of a vast range of biomarkers.
关键词： Biomarkers,Fluorescence detection,Silicon photomultiplier
更新于2025-09-09 09:28:46
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A Naphthalimide-Based Fluorescent Turn-On Sensor for the Selective Detection of Diethyl Chlorophosphate
摘要： A naphthalimide derivative, named 4-(2-aminophenoxy)-N-(2-ethylhexyl)-naphthalimide (AEN), was synthesized and characterized by NMR and ESI-MS. The photophysical behaviors of AEN toward diethyl chlorophosphate (DCP) were investigated by fluorescence spectrometry. Moreover, the detection mechanism of AEN for DCP was evaluated by 1H, 31P NMR and ESI-MS spectroscopy. These results show that the fluorescence intensity of AEN significantly increased with the augment of DCP concentration. No obvious interference with the DCP detection was observed in the presence of other common solvents (e. g. tetrahydrofuran, dimethyl sulfoxide, n-hexane, N,N-dimethylformamide, and isopropanol), phosphorus-containing reagents (e. g. dimethyl methylphosphonate, and triethyl phosphate), brominated compounds (1-bromobutane) and chlorinated compounds (e. g. 1,2-dichloroethane, chlorobenzene, carbon tetrachloride), indicating that AEN possesses satisfactory selectivity and sensitivity for the detection of DCP.
关键词： Fluorescence,Detection,Diethyl chlorophosphate,Naphthalimide
更新于2025-09-04 15:30:14