研究目的
Investigating the application of silicon photomultipliers (SiPM) in the fluorescence detection of biomarkers, aiming to develop a portable device capable of detecting low concentrations of biomarkers in small sample volumes.
研究成果
The SiPM was successfully applied to fluorescence detection of biomarkers, with the lowest detectable concentrations being 12.7 pg/ml in a stationary system and 1.47 pg/ml in a microfluidic system. For the Anti-NPR1 biomarker, the lowest concentration measured was 0.55 pg/ml. The system's adaptability allows for the detection of a wide range of biomarkers, indicating its potential for portable diagnostic applications.
研究不足
The study was limited by the minimum and maximum concentrations that could be detected, defined by the LOD parameter. The application, while adaptable to multi-detector measurements, initially used only a single photodetector.
1：Experimental Design and Method Selection:
The study utilized a silicon photomultiplier (SiPM) for detecting fluorescence in biomarkers, employing flow cytometry and single molecule detection methods. A microfluidic system was used to minimize sample volume.
2：Sample Selection and Data Sources:
Samples were prepared from base concentrations of fluorophores (fluorescein and CF488) diluted in Tris buffer, with subsequent samples being 10-fold smaller in concentration.
3：List of Experimental Equipment and Materials:
Equipment included a 488 nm laser diode, UV–VIS optic fibre, photometric cuvette, syringe pump, and a 530 nm band-pass optic filter.
4：Experimental Procedures and Operational Workflow:
Fluorescence was triggered by laser pulses delivered via optic fibre to the fluorophore in either stationary or microflow optical systems. The fluorescence intensity was measured over predefined time intervals.
5：Data Analysis Methods:
The limit of detection (LOD) was calculated to define the minimum detectable concentration with a given confidence limit.
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