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Single Component Self-Assembled Thermally Activated Delayed Fluorescence Nanoprobe
摘要： A novel versatile thermally activated delayed fluorescence (TADF) nanoprobe, AI-Cz-NP, was designed and fabricated through self-assembly of the single-component amphiphilic monomer for potential applications in confocal imaging and time-resolved fluorescence imaging.
关键词： nanoprobe,self-assembly,thermally activated delayed fluorescence,confocal imaging,time-resolved fluorescence imaging
更新于2025-09-16 10:30:52
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Near infrared molybdenum oxide quantum dots with high photoluminescence and photothermal performance
摘要： The synthesized near infrared molybdenum oxide quantum dots perform excellent red fluorescence imaging performance and photothermal performance, which have 600, 650 and 700 nm three unique peaks excited at 540 nm, with a high quantum yield around 20%. Meanwhile, with 808 nm NIR laser excitation, 10 mg/mL modified Molybdenum oxide quantum dots can increase temperature up to 72.2 oC within 150 s and 77.7 oC within 270 s, respectively.
关键词： Fluorescence imaging performance,Molybdenum oxide quantum dots,Photothermal performance
更新于2025-09-16 10:30:52
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Fluorescence imaging analysis of depth‐dependent degradation in photovoltaic laminates: insights to the failure
摘要： Accurate evaluation of the reliability of photovoltaic (PV) packaging materials is critically important for the long‐term safe operation of modules. However, the complexity of the laminated systems due to their multilayered and multicomponent structures and diverse aging mechanisms makes a thorough system evaluation very challenging, especially when the degradation is non‐uniform through the thickness. In such a case, neither surface nor bulk measurements can present a clear picture of the degradation profile. In this study, fluorescence imaging was developed to visualize the degradation depth‐profiles of an aged laminated PV system. A glass/ethylene vinyl acetate (EVA) encapsulant/poly(ethylene terephthalate) (PET)‐PET‐EVA (PPE) backsheet laminate was weathered with the glass‐side facing an ultra-violet (UV) light source for 3840 h. Cross‐sectional fluorescence images revealed a non‐uniform distribution of degradation species across the thickness of the EVA encapsulant, providing greater insight into the mechanisms of degradation, which are unavailable by traditional bulk‐based methods. In addition, strong fluorescence emissions were observed from the two thin adhesive layers of the aged backsheet, indicating severe degradation of the adhesives and a potential for interlayer delamination. This method is further confirmed with other microscale characterization techniques. The changes in optical (yellowness index), chemical (oxidation, UV absorber concentration), mechanical (Derjaguin‐Muller‐Toporov modulus), and thermal (melting enthalpy) properties of the EVA encapsulant were found to be related to fluorescence profiles, following the attenuation of UV light. This study highlights that fluorescence imaging is a spatially‐resolved and sensitive method for rapid failure assessment and in‐depth mechanism study for complex PV‐laminated system.
关键词： modulus,depth profile,UV aging,photovoltaic laminates,yellowness index,fluorescence imaging,degradation
更新于2025-09-16 10:30:52
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In Vivo Fluorescence Visualization of Anterior Chamber Injected Human Corneal Endothelial Cells Labeled With Quantum Dots
摘要： The injection of cultured human corneal endothelial cells (cHCECs) into the anterior chamber (AC) is a newly developed modality for the successful treatment of corneal endothelium dysfunction. Here, we investigated whether or not cHCECs could be labeled using quantum dots (QDs) composed of semiconductor nanoparticle octa-arginine (R8) to trace injected cHCECs and examined the utility of in vivo fluorescence imaging to analyze the dynamics and accumulation of QD-labeled injected cHCECs in a corneal endothelial dysfunction mouse model.
关键词： cultured human corneal endothelial cells (cHCECs),in vivo fluorescence imaging,quantum dots (QDs)
更新于2025-09-16 10:30:52
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Covalent Surface Modification Effects on Singlea??Walled Carbon Nanotubes for Targeted Sensing and Optical Imaging
摘要： Optical nanoscale technologies often implement covalent or noncovalent strategies for the modification of nanoparticles, whereby both functionalizations are leveraged for multimodal applications but can affect the intrinsic fluorescence of nanoparticles. Specifically, single-walled carbon nanotubes (SWCNTs) can enable real-time imaging and cellular delivery; however, the introduction of covalent SWCNT sidewall functionalizations often attenuates SWCNT fluorescence. Recent advances in SWCNT covalent functionalization chemistries preserve the SWCNT’s pristine graphitic lattice and intrinsic fluorescence, and here, such covalently functionalized SWCNTs maintain intrinsic fluorescence-based molecular recognition of neurotransmitter and protein analytes. The covalently modified SWCNT nanosensor preserves its fluorescence response towards its analyte for certain nanosensors, presumably dependent on the intermolecular interactions between SWCNTs or the steric hindrance introduced by the covalent functionalization that hinders noncovalent interactions with the SWCNT surface. These SWCNT nanosensors are further functionalized via their covalent handles with a targeting ligand, biotin, to self-assemble on passivated microscopy slides, and these dual-functionalized SWCNT materials are explored for future use in multiplexed sensing and imaging applications.
关键词： single-walled carbon nanotubes,biosensing,fluorescence,imaging,surface functionalization
更新于2025-09-16 10:30:52
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Biodegradable and Photostable Nb2C MXene Quantum Dots as Promising Nanofluorophores for Metal Ions Sensing and Fluorescence Imaging
摘要： Conventional fluorescent probes are either easily photobleached or non-biodegradable, which often leads to the unstable fluorescence signal output and long-term biological toxicity. Therefore, the development of novel fluorescent materials with both excellent photostability and biodegradability is of great significance for further broadening their application in numerous research fields. In this work, Nb2C quantum dots (Nb2C QDs) with pristine crystallographic structures of Nb2C MXene phases and surface oxygen-containing species are synthesized by an ultrasound assisted physicochemical exfoliation in tetrapropylammonium hydroxide. Detailed analyses indicate that the Nb2C QDs not only possess excellent chemical- and photo-stable fluorescence emission but also achieve successful application in fluorescence sensing of heavy metal ions and fluorescence imaging. More importantly, it is confirmed that the Nb2C QDs present high biocompatibility and unique biodegradation property responsive to human myeloperoxidase, implying the application safety of Nb2C QDs in vivo. Under the background of ever-growing and stringent requirements for biosafety and technical stability, the biocompatibility, biodegradability, and most importantly the promising fluorescence sensing/imaging characteristic of the obtained Nb2C QDs may argue well for their future applications in environmental monitoring, biomedical diagnosis, visual display, and anti-counterfeiting.
关键词： MXene quantum dots,biodegradation,fluorescent probes,fluorescence imaging,photobleaching resistance
更新于2025-09-16 10:30:52
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Laser-induced forward transfer of soft material nanolayers with millisecond pulses shows contact-based material deposition
摘要： In this work, we present a qualitative and quantitative experimental analysis, as well as a numerical model, of a novel variant of the laser-induced forward transfer, which uses millisecond laser pulses. In this process, soft material nanolayer spots are transferred from a donor slide, which is coated with the soft material layer, to an acceptor slide via laser irradiation. This method offers a highly flexible material transfer to perform high-throughput combinatorial chemistry for the generation of biomolecule arrays. For the first time, we show visual evidence that the main transfer mechanism is contact-based, due to thermal surface expansion of the donor layer. Thus, the process is different from the many known variants of laser-induced forward transfer. We will characterize the maximum axial surface expansion in relation to laser power and pulse duration. On this basis, we derive a numerical model that approximates the axial surface expansion within measurement tolerances. Finally, we analyze the topology of the transferred soft material nanolayer spots by fluorescence imaging and vertical scanning interferometry to determine width, height, and shape of the transferred material. Concluding from this experimental and numerical data, we can now predict the amount of transferred material in this process.
关键词： high-speed imaging,fluorescence imaging,experimental and numerical prediction,vertical scanning interferometry,OpenFOAM
更新于2025-09-12 10:27:22
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Shining Light on the Coiled-Flow Inverter—Continuous-Flow Photochemistry in a Static Mixer
摘要： We present the use of a coiled-flow inverter (CFI) for continuous-flow photochemistry at competitive photon efficiencies. The static mixer is placed inside a reaction chamber, while a dark adjacent chamber allows for orthogonal online reaction monitoring via fluorescence spectroscopy. The study of the aqueous visible-light induced degradation of fluorescein with ZnO-APTMS-Au photocatalyst showcases the challenge of uniformly irradiating photoreactors with non-planar surfaces. Fluorescence imaging is introduced as a simple method to visualize spatial gradients in the irradiance at the outer surface of such complex photoreactor geometries, allowing the analysis of photoreactor efficiency as a function of lighting configuration. We compared uniaxial and multiaxial lighting configurations and discuss the challenges associated with attaining uniform irradiance distribution of incident light on coiled-flow inverters, where chaotic advection combats light attenuation. A first calculation of the photochemical space-time yield (PSTY) for a “photo-CFI” is presented and contrasted with other photoreactor designs.
关键词： continuous-flow photochemistry,coiled-flow inverter,fluorescence imaging,ZnO-APTMS-Au photocatalyst,photochemical space-time yield
更新于2025-09-12 10:27:22
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Noninvasive In Vivo Imaging in the Second Near-Infrared Window by Inorganic Nanoparticle-Based Fluorescent Probes
摘要： The fluorescence imaging in the second near-infrared window (NIR-II, 1000-1700 nm) has emerged as a new method for in vivo imaging and attracted considerable attention in the past decade. Owing to the suppressed photon scattering and diminished autofluorescence, in vivo fluorescence imaging in NIR-II window can afford deep tissue penetration depth with high clarity. Inorganic nanoparticle-based fluorescent probes in NIR-II window have greatly prospered the field into a development stage because of their superior traits, including adjustable emission covering the whole NIR-II window, and abundant surface functional groups that facilitate chemical modification and bioconjugation, etc. In this feature article, we introduce the unique imaging performance of the NIR-II optical window, and highlight the latest development of noninvasive biological fluorescent imaging in NIR-II window using inorganic nanoparticle-based probes. A perspective on the challenge and future direction of inorganic nanoparticle-based NIR-II probes is also discussed.
关键词： noninvasive imaging,NIR-II,inorganic nanoparticles,fluorescence imaging,in vivo imaging
更新于2025-09-11 14:15:04
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An Organelle-redirected Chameleon Sensor Enabled Live Cell Imaging of Mitochondrial DNA
摘要： Mitochondrial DNA (mtDNA) plays important roles in diverse physiological processes and myriad diseases. We herein report mtDNA imaging with a chameleon sensor containing a cationic rhodamine B (RB) entity for mitochondria targeting and a fluorogenic SYBR Green-I (SG) entity for DNA sensing. SG-RB selectively binds to mtDNA and gives green SG fluorescence in mitochondria of living cells but gives red RB fluorescence upon delivery of mitochondria into lysosomes in mitophagy. With the dual color imaging, mtDNA aggregation and elevated mitophagy were identified in HeLa cells stressed with anticancer doxorubicin. These results suggest the utility of organelle-redirected DNA sensors for live cell imaging of mtDNA involved in myriad pathological disorders.
关键词： Mitochondrial DNA,Fluorescence imaging,Color switch,Autophagy,Organelle targeting
更新于2025-09-11 14:15:04