研究目的
To design and fabricate a novel versatile thermally activated delayed fluorescence (TADF) nanoprobe for potential applications in confocal imaging and time-resolved fluorescence imaging.
研究成果
The study successfully designed and constructed a novel single-component TADF nanoprobe through self-assembly of an amphiphilic aromatic-imide-based TADF monomer. The nanoprobe exhibited excellent dispersibility in aqueous solution, low oxygen sensitivity, and preserved long lifetime emission in any environment, making it desirable for confocal imaging and TRFI.
研究不足
The sensitivity of the excited triplet state in TADF emitters to surrounding oxygen presents challenges for wide application in time-resolved fluorescence imaging (TRFI).
1：Experimental Design and Method Selection:
The study involved the design and synthesis of an amphiphilic monomer (AI-Cz-AM) that self-assembles into nanoparticles (AI-Cz-NP) in aqueous solutions. The methodology included the use of aggregation-induced emission (AIE) behavior to enhance fluorescence intensity.
2：Sample Selection and Data Sources:
The samples included AI-Cz-AM and its self-assembled nanoparticles AI-Cz-NP, characterized using various spectroscopic and microscopic techniques.
3：List of Experimental Equipment and Materials:
Equipment included scanning electron microscope (SEM), transmission electron microscopy (TEM), Dynamic light scattering (DLS), and confocal laser scanning microscopy (CLSM). Materials included THF, water, and the synthesized amphiphilic monomer.
4：Experimental Procedures and Operational Workflow:
The nanoprobe was prepared by reprecipitation method, and its properties were characterized through fluorescence spectroscopy, SEM, TEM, DLS, and CLSM.
5：Data Analysis Methods:
Data analysis involved fitting photoluminescence decay curves to biexponential decay models and calculating singlet-triplet energy differences (ΔEST).
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
