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Boron doped carbon dots as a multifunctional fluorescent probe for sorbate and vitamin B12
摘要： Boron doped carbon dots (B-CD) were synthesized by a one-step hydrothermal method using phenylboronic acid as the starting material. They have an average size of about 3.3 nm, with excitation/emission wavelength of 247/323 nm and a quantum yield of 12%. The B-CD is shown to be viable fluorescent probe for sorbate (PS) and vitamin B12 (VB12). The fluorescence (FL) of the B-CD is quenched in the presence of PS or VB12 mainly coming from inner filter effect (IFE), but F?rster resonance energy transfer (FRET) from the B-CD (as a donor) to PS/VB12 (as an acceptor) cannot be excluded. The probe enables PS to be detected by fluorometry with a linear response in the 0.20–24 μM concentration range and a 6.1 nM detection limit (at 3σ/slope). For VB12, the data are 0.20–30 μM and 8.0 nM.
关键词： Fluorescent probe,Sorbic acid,Fluorescence quenching ratios,Intra-day precision,Selectivity,Inter-day precision,Quenching mechanism,Real sample analysis
更新于2025-09-23 15:23:52
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Enhancement strategies of targetability, response and photostability for in vivo bioimaging
摘要： Analyses of the physiology and pathology of active biochemical species in their native contexts are critical for early diagnosis and therapy. Optical imaging has emerged as one of the promising modalities for noninvasive and real-time visualization of important biomolecules or biological events, and it has witnessed major advances in the field of imaging in vitro and in vivo. In this review, we present a survey of common approaches and tactics for enhanced targetability, response rate, and photostability in bioimaging applications. Recently developed and representative examples are illustrated on the cellular and tissue levels.
关键词： targetability,bioimaging,photostability,response rate,fluorescent probe
更新于2025-09-23 15:23:52
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Construction of a Novel Fluorescent Probe for On-site Measuring Hydrogen Sulfide Levels in Food Samples
摘要： Hydrogen sulfide (H2S) has been involved in many foodstuffs and beverages. On-site measuring the H2S level in food samples was beneficial to the concerns about food safety and food quality. Herein, we rationally constructed a novel fluorescent probe to track the H2S levels in various food samples. The optical experiments indicated that the probe displayed highly selective and sensitive response to H2S. In addition, the probe can function well in a wide pH value condition, providing a reliable platform to sense H2S. The practical application demonstrated that the probe was applied for tracking H2S levels in banana, grape, and vinegar for the first time. Importantly, by fabricating test strips, the probe was first served to monitor the released H2S in raw meat without relying on elaborate instrumentation. Thus, the probe could be employed as a simple tool to measure the H2S levels in various food samples, as well as to monitor the fresh-like quality of raw meat under on-site conditions.
关键词： Fluorescent probe,On-site measurement,Hydrogen sulfide,Food sample,Test strip
更新于2025-09-23 15:23:52
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Peptide-based ratiometric fluorescent probe for highly selective detection of Cd (II) and its application in bioimaging
摘要： This work reported a new peptide fluorescent probe L including dansyl group (acceptor) and tryptophan (donor) via imitated the binding sites of natural protein. L exhibited exclusively selective ratiometric fluorescence response towards Cd2+ ions over other metal ions based on fluorescent resonance energy transfer (FRET) effects. As designed, L showed exciting sensitivity to Cd2+ ions in aqueous media, and the detection limits are less than 27.5 nM. In addition, the binding pattern of L and Cd2+ was confirmed by fluorescence titration analysis which was found to be 2:1 and further it was confirmed by Job's plot. What' more, L exhibited very low biotoxicity and exciting cell infiltration, and have been successfully used for fluorescence imaging of Cd2+ ions in living HeLa cells.
关键词： Cell imaging,Fluorescent probe,Peptide,Cadmium ion,Ratiometric
更新于2025-09-23 15:23:52
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Facile method for iodide ion detection via the fluorescence decrease of dihydrolipoic acid/beta-cyclodextrin protected Ag nanoclusters
摘要： In this work, novel photoluminescent Ag nanoclusters (Ag NCs) with red emission are synthesized and successfully used for detecting iodide ion (I?). The dihydrolipoic acid (DHLA) is used as the stabilizing agent and beta-cyclodextrin (β-CD) is used as the auxiliary stabilizing agent. DHLA and β-CD are combined with Ag atoms by the formation of Ag-S bonds and hydrophobic interaction, respectively. Functionalization of β-CD endows good photoluminescent properties and solubility in water to the Ag NCs. The obtained DHLA and β-CD-protected Ag NCs (DHLA/β-CD-Ag NCs) are spherical and display a dispersed state. However, the DHLA/β-CD-Ag NCs are aggregated in the presence of I?, accompanied by the decrease in their fluorescence intensity. Because the integrity of β-CD cavities is retained on the surface of DHLA/β-CD-Ag NCs, which preserves their capability for I? host–guest recognition, the DHLA/β-CD-Ag NCs combine with I? through the formation of inclusion complexes. Based on this phenomenon, the prepared DHLA/β-CD-Ag NCs can be designed as a novel fluorescent probe for I? detection. The limit of detection (LOD) is calculated as 0.06 μM, indicating that it is an ideal probe for I? detection in practical applications.
关键词： Ag nanoclusters,Fluorescent probe,Beta-cyclodextrin,Dihydrolipoic acid,Iodide ion
更新于2025-09-23 15:23:52
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A coumarin-based colorimetric fluorescent probe for rapid response and highly sensitive detection of hydrogen sulfide in living cells
摘要： Hydrogen sulfide (H2S) plays a vital role in numerous biological processes in living organisms. To better understand its functions, a fluorescent probe to fast and sensitively detect H2S is imminently needed. Keep this in mind, we reasonably designed probe DHC for detecting H2S based on α, β-unsaturated ethanoylcoumarin fluorophore. The limit of detection (LOD) is found to be as low as 5 × 10?8 M, which is superior to most reported fluorescent probes to detect H2S. Furthermore, the wide pH range of 4–11 makes it capable of application in biological systems. Most importantly, MTT assays and cell imaging experiments indicate that probe DHC has hypotoxicity and outstanding membrane permeability, which makes DHC successful imaging of H2S in Baby Hamster Syrian Kidney (BHK) cells.
关键词： Cell-imaging,Fluorescent probe,Rapid response,H2S,Coumarin
更新于2025-09-23 15:23:52
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A turn-on reactive fluorescent probe for Hg2+ in 100% aqueous solution
摘要： It is an effective and precise strategy to design the fluorescence probe for Hg2+ based on a new specific reaction. A novel turn-on fluorescent probe, 4-{2-[4-(2-Mercapto-benzoyloxy)-phenyl]-vinyl}-1-methyl-pyridinium[e]iodide (MBPVP) for Hg2+ was developed. The mechanism was based on Hg2+-mediated ester hydrolysis to release the fluorophore, 4-[2-(4-Hydroxy-phenyl)-vinyl]-1-methyl-pyridinium[e]iodide, which showed outstanding intramolecular charge transfer (ICT) and the yellow-green fluorescence were observed. The turn-on probe showed rapid response time, outstanding selectivity and sensitivity for Hg2+ over other metal ions in 100% PBS buffer (pH = 7.4). The linear range was 2-16 μM and the detection limit was 6.5 nM. Furthermore, MBPVP was used to determine Hg2+ in real samples and in the solid state.
关键词： Hg2+,ester hydrolysis,MBPVP,100% aqueous solution,fluorescent probe
更新于2025-09-23 15:23:52
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Rational Design of Near-Infrared AIE-Active Probes: In Situ Mapping of Amyloid-β Plaques with Ultra-Sensitivity and High-Fidelity
摘要： High-fidelity mapping of amyloid-β (Aβ) plaques is critical for the early detection of Alzheimer’s disease. However, in vivo probing of Aβ plaques by commercially available Thioflavin derivatives (ThT or ThS) has proven to be extremely limited, as evident by the restriction of enrichment quenching effect, low signal-to-noise (S/N) ratio and poor blood-brain barrier (BBB) penetrability. Herein, we demonstrate a rational design strategy of near-infrared (NIR) aggregation-induced emission (AIE)-active probes for Aβ plaques, through introducing lipophilic π-conjugated thiophene-bridge for extending to NIR wavelength with enhancement of BBB penetrability, and tuning the substituted position of sulfonate group for guaranteeing specific hydrophilicity to keep fluorescence-off state before binding to Aβ deposition. Probe QM-FN-SO3 has well settled the AIE dilemma between lipophilic requirement for longer emission and aggregation behavior from water to protein fibrillogenesis, thus making a breakthrough in high-fidelity feedback on in vivo detection of Aβ plaques with remarkable binding affinity, and serving as an efficient alternative to the commercial probe ThT or ThS.
关键词： Fluorescent probe,In vivo imaging,Aggregation-induced emission,Amyloid-β plaques,Near-infrared
更新于2025-09-23 15:23:52
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A new lysosome-targetable fluorescent probe with a large Stokes shift for detection of endogenous hydrogen polysulfides in living cells
摘要： Hydrogen polysulfides (H2Sn, n>1) influence a variety of important biological functions and activities associated with hydrogen sulfide (H2S). The development of probes for rapid, selective, and sensitive detection of H2Sn still remains a great challenge. Lysosome plays crucial roles in various physiological processes among living cells, which arouse high interest in detecting endogenous lysosome-targetable H2Sn. To the best of our knowledge, there is no lysosome-targetable probe for H2Sn has been reported. In this work, a new lysosome-targetable probe NIPY-NF, based on the scaffold of imidazo[1,5-α]pyridine, with a large Stokes shift (215 nm), low detection limit (84 nM), fast response time (6 min) and low cytotoxicity was designed and synthesized. Furthermore, NIPY-NF was successfully applied into the cell imaging for detection of endogenous lysosome-targetable H2Sn.
关键词： Hydrogen polysulfides,Fluorescent probe,Lysosome,Cell imaging,Imidazo[1,5-α]pyridine
更新于2025-09-23 15:23:52
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Ratiometric fluorescence imaging for sodium selenite in living cells
摘要： Sodium selenite as a common selenium-containing agent has been extensively studied for human health, especially cancer prevention and treatment. However, precisely evaluating the dynamic changes of sodium selenite at cellular and molecular level in these pharmacological and pathophysiological studies is still challenging. Developing the chemical tools that enable the quantification of sodium selenite in vivo with high spatial and temporal fidelity thus can provide an efficient solution to this challenge. Here, we report the design, synthesis and biological evaluation of ratiometric sodium selenite fluorescent probe (HBTN-Se) for quantitative ratiometric fluorescence imaging of sodium selenite concentration fluctuations in living cells. A strategy for sodium selenite quantification has been achieved effectively through using a highly selective, rapid and biocompatible hydroxyl-deprotecting reaction. The ratiometric fluorescence imaging with HBTN-Se is constructed to precisely monitor changes in sodium selenite levels in living cells. Moreover, HBTN-Se is capable of visualizing differences in sodium selenite levels between normal cells and cancer cells, establishing the utility of this ratiometric detection platform for assessing the correlation between physiological effects of sodium selenite and its levels in living cells.
关键词： Quantitative determination,Fluorescent probe,Sodium selenite,Ratiometric fluorescence imaging
更新于2025-09-23 15:22:29