研究目的
Developing a facile method for iodide ion detection using fluorescence decrease of dihydrolipoic acid/beta-cyclodextrin protected Ag nanoclusters.
研究成果
The DHLA/β-CD-Ag NCs were successfully synthesized and demonstrated as an effective fluorescent probe for I? detection with high sensitivity (LOD of 0.06 μM) and selectivity. The method is facile, cost-effective, and applicable to real samples like milk and drugs, showing promise for practical use in iodide detection.
研究不足
The method may have limitations in terms of interference from other ions, although selectivity tests showed minimal interference. The synthesis requires specific conditions like low temperature (0°C) and long incubation times, which could be optimized for faster processing. Application in complex real samples might require extensive pretreatment.
1：Experimental Design and Method Selection:
The study involves synthesizing Ag nanoclusters (Ag NCs) using dihydrolipoic acid (DHLA) and beta-cyclodextrin (β-CD) as stabilizing agents, with sodium borohydride (NaBH4) as the reducing agent. The method is designed to leverage the host-guest recognition between β-CD cavities and iodide ions (I?) for fluorescence-based detection.
2：Sample Selection and Data Sources:
Reagents such as alpha-, beta-, gamma-cyclodextrin, silver nitrate, sodium borohydride, lipoic acid, potassium iodide, and other salts were purchased from specified suppliers. Real samples include milk and drug samples (cydiodine buccal tablets).
3：List of Experimental Equipment and Materials:
Equipment includes a Shimadzu UV-vis 2450 spectrophotometer, Hitachi F-2700 fluorescence spectrophotometer, JEM-2100 JEOL transmission electron microscope, thermo Fisher Scientific ESCALAB 250Xi system for XPS, thermo Nicolet NEXUS 670 spectrometer for FT-IR, and a PHS-3C pH meter. Materials include various chemicals and ultrapure water.
4：Experimental Procedures and Operational Workflow:
Synthesis of DHLA/β-CD-Ag NCs involves reducing lipoic acid to DHLA, adding β-CD and AgNO3, then NaBH4, with stirring and incubation. Detection of I? involves adding PBS solutions with I? to the Ag NCs solution, incubating, and measuring fluorescence. Sample pretreatment for milk and drug involves centrifugation and other steps.
5：Data Analysis Methods:
Fluorescence intensity measurements, UV-vis absorption spectra, TEM for size distribution, XPS and FT-IR for surface composition, and statistical analysis for LOD and linear range.
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