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A long-wavelength-emitting fluorescent probe for simultaneous discrimination of H2S/Cys/GSH and its bio-imaging applications
摘要： A long-wavelength fluorescent probe NR-CY was developed for simultaneous identification of cysteine / glutathione and sulphide by combining the derivative of Nile red with 7-nitrobenzofurazan. The response of NR-CY to thiols is regulated by intramolecular charge transfer and photoinduced electron transfer mechanisms. For sulphide at 560 nm, cysteine at 475 nm and glutathione at 425 nm, different absorbance increases can be observed. NR-CY can detect cysteine at fluorescence emission 543 nm and distinguish sulphide from other analytes by kinetic experiments at 636 nm. The probe showed a rapid response to these thiols (cysteine was 90 s and sulphide was 30 s). In addition, NR-CY has been successfully applied to live MCF-7 cell imaging.
关键词： biothiols,Fluorescent probe,cell imaging,long-wavelength-emitting
更新于2025-09-23 15:19:57
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Cancer-Specific Biomarker hNQO1-Activatable Fluorescent Probe for Imaging Cancer Cells In Vitro and In Vivo
摘要： Human NAD(P)H quinone oxidoreductase-1 (hNQO1) is an important cancer-related biomarker, which shows significant overexpression in malignant cells. Developing an effective method for detecting NQO1 activity with high sensitivity and selectivity in tumors holds a great potential for cancer diagnosis, treatment, and management. In the present study, we report a new dicyanoisophorone (DCP) based fluorescent probe (NQ-DCP) capable of monitoring hNQO1 activity in vitro and in vivo in both ratiometric and turn-on model. NQ-DCP was prepared by conjugating dicyanoisophorone fluoroprobe with hNQO1 activatable quinone propionic acid (QPA), which remain non-fluorescent until activation by tumor-specific hNQO1. NQ-DCP featured a large Stokes shift (145 nm), excellent biocompatibility, cell permeability, and selectivity towards hNQO1 allowed to differentiate cancer cells from healthy cells. We have successfully employed NQ-DCP to monitor non-invasive endogenous hNQO1 activity in brain tumor cells in vitro and in xenografted tumors developed in nude mice.
关键词： NAD(P)H quinone oxidoreductase 1,cancer biomarker,tumor diagnosis,fluorescent probe,cancer imaging,large Stokes shift,dicyanoisophorone
更新于2025-09-19 17:15:36
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Imaging of anti-inflammatory effects of HNO <i>via</i> a near-infrared fluorescent probe in cells and in rat gouty arthritis model
摘要： Nitroxyl (HNO) plays a crucial role in anti-inflammatory effects via the inhibition of inflammatory pathways, but the details of the endogenous generation of HNO still remain challenging owing to the complex biosynthetic pathways, in which the interaction between H2S and NO simultaneously generates HNO and polysulfides (H2Sn) in mitochondria. Moreover, nearly all the available fluorescent probes for HNO are utilized for imaging HNO in cells and tissues, instead of the in situ real-time detection of the simultaneous formation of HNO and H2Sn in mitochondria and animals. Here, we have developed a mitochondria-targeting near-infrared fluorescent probe, namely, Mito-JN, to detect the generation of HNO in cells and a rat model. The probe consists of three moieties: Aza-BODIPY as a fluorescent signal transducer, a triphenylphosphonium cation as a mitochondria-targeting agent, and a diphenylphosphinobenzoyl group as an HNO-responsive unit. The response mechanism is based on an aza-ylide intramolecular ester aminolysis reaction with fluorescence emissions on. Mito-JN displays high selectivity and sensitivity for HNO over various other biologically relevant species. Mito-JN was successfully used for the detection of the endogenous generation of HNO, which is derived from the crosstalk between H2S and NO in living cells. The additional generation of H2Sn was also confirmed using our previous probe Cy-Mito. The anti-inflammatory effect of HNO was examined in a cell model of LPS-induced inflammation and a rat model of gouty arthritis. The results imply that our probe is a good candidate for the assessment of the protective effects of HNO in inflammatory processes.
关键词： H2S,fluorescent probe,HNO,polysulfides,gouty arthritis,anti-inflammatory,near-infrared,NO,mitochondria-targeting
更新于2025-09-19 17:15:36
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A Turn-On Fluorescent Probe for Sensitive Detection of Cysteine in a Fully Aqueous Environment and in Living Cells
摘要： We reported here a turn-on fluorescent probe (1) for the detection of cysteine (Cys) by incorporating the recognition unit of 2,4-dinitrobenzenesulfonyl ester (DNBS) to a coumarin derivative. The structure of the obtained probe was confirmed by NMR and HRMS techniques. The probe shows a remarkable fluorescence off-on response (～52-fold) by the reaction with Cys in 100% aqueous buffer. The sensing mechanism was verified by the HPLC test. Probe 1 also displays high selectivity towards Cys. The detection limit was calculated to be 23 nM. Moreover, cellular experiments demonstrated that the probe is highly biocompatible and can be used for monitoring intracellular Cys.
关键词： living cells,aqueous environment,fluorescent probe,turn-on,cysteine
更新于2025-09-19 17:15:36
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A highly selective and sensitive fluorescent probe for simultaneously distinguishing and sequentially detecting H <sub/>2</sub> S and various thiol species in solution and in live cells
摘要： A novel dual-channel fluorescent probe (NCR) based on differences in reactivity among H2S, Cys/Hcy, and GSH was rationally designed for simultaneously distinguishing and sequentially sensing H2S, Cys/Hcy, and GSH using two emission channels, which also demonstrated that NCR can be used for targeting mitochondria in mammalian cells.
关键词： live cells,thiol species,fluorescent probe,mitochondria-targeted,H2S
更新于2025-09-19 17:15:36
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Towards Detection of Glycoproteins Using Molecularly Imprinted Nanoparticles and Boronic Acid-Modified Fluorescent Probe
摘要： Glycoproteins represent a group of important biomarkers for cancer and other life-threatening diseases. Selective detection of specific glycoproteins is an important step for early diagnosis. Traditional glycoprotein assays are mostly based on lectins, antibodies, and enzymes, biochemical reagents that are costly and require special cold chain storage and distribution. To address the shortcomings of the existing glycoprotein assays, we propose a new approach using protein-imprinted nanoparticles to replace the traditional lectins and antibodies. Protein-imprinted binding sites were created on the surface of silica nanoparticles by copolymerization of dopamine and aminophenylboronic acid. The imprinted nanoparticles were systematically characterized by dynamic light scattering, scanning and transmission electron microscopy, thermogravimetric analysis, Fourier transform infrared spectroscopy, and elemental analysis. A boronic acid-modified fluorescent probe was used to detect the target glycoprotein captured by the imprinted nanoparticles. Using horseradish peroxidase as a model glycoprotein, we demonstrated that the proposed method can be applied to detect target protein containing multiple glycosylation sites. Because of their outstanding stability and low cost, imprinted nanoparticles and synthetic probes are attractive replacements of traditional biochemical reagents to develop simpler, faster, and more cost-effective analytical methods for glycoproteins.
关键词： glycoprotein,molecular imprinting,boronic acid,fluorescent probe
更新于2025-09-19 17:15:36
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A selective and sensitive azido near-infrared fluorescent probe for tris(2-carboxyethyl)phosphine quantitative detection and its application for <i>E. coli</i> determination
摘要： We developed a sensitive near-infrared fluorescent probe, azido hemicyanine (HC-N3), for the detection of tris(2-carboxyethyl)phosphine (TCEP) with the lowest LOD (92 nM) so far. HC-N3 has good selectivity for TCEP among thiols and 20 kinds of natural amino acid. The quantification of E. coli (101–103 cfu mL?1) demonstrated its practicability in complicated biosystems.
关键词： E. coli determination,near-infrared fluorescent probe,selectivity,sensitivity,TCEP detection
更新于2025-09-19 17:15:36
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Design and synthesis of a novel colorimetric fluorescent probe for selective detection of sulfur dioxide in SH-SY5Y neuroblastoma cells and its application in Traditional Chinese Medicines
摘要： Sulfur fumigation has attracted more and more attention as one of the important post-harvest processing methods for some Traditional Chinese Medicines (TCMs) in the last decade. However, sulfur-fumigated TCMs have recently emerged as a controversial topic due to their potential detrimental effect on the safety and efficacy, as some sulfur-fumigated TCMs contain lots of sulfur dioxide derivatives. Additionally, high levels of the sulfur dioxide derivatives could cause some diseases and dangerous environmental pollutant. In this work, a fast response time, low limit of detection and high fluorescence quantum yield probe DTCC was designed and synthesized to detect SO2 derivatives based on coumarin-thiophene dye which was fused with a coumarin moiety and 2-thiophenecarboxaldehyde. Probe DTCC exhibited fast response time (less than 10 s), satisfactory selectivity for SO2 derivatives in the presence of other ROS and excellent sensitivity for SO2 derivatives with low limit of detection (0.23 μM) and widely linear range (0～100 μM). Furthermore, probe DTCC was successfully applied in fluorescent imaging in SH-SY5Y neurobalstoma cells with excellent membrane permeability and stability. It was also employed for monitoring the total SO2 derivatives in several real TCMs sample. These results illustrated that probe DTCC has an excellent capability for monitoring SO2 derivatives in living cells and real TCMs sample.
关键词： Fast responsive,Fluorescent probe,Coumarin scaffold,Bioimaging,Sulfur dioxide,Traditional Chinese Medicines
更新于2025-09-19 17:15:36
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A novel and simple imidazo[1,2-a]pyridin fluorescent probe for the sensitive and selective imaging of cysteine in living cells and zebrafish
摘要： Cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) play many crucial physiological roles in organisms. Their abnormal levels can cause and indicate various diseases. In the present study, a small-molecule fluorescent probe 2-(imidazo[1,2-a]pyridin-2-yl)phenyl acrylate (IPPA) was designed, synthesized and characterized by NMR, FT-IR and HRMS. IPPA can selectively detect Cys over other analytes because of an approximately 76 times enhancement in fluorescence intensity. The limit of detection of IPPA for Cys was 0.33 μM. The pseudo-first-order rate constant of the reaction between IPPA and Cys was approximately 10 times that of the reaction between IPPA and Hcy (KCys 3.18 × 10?? S?1 vs KHcy 4.92 × 10?? S?1), indicating that Cys can be distinguished from Hcy. In addition, IPPA exhibits strong anti-interference ability, small molecular weight, high efficiency, low toxicity and good cell permeability. It was successfully used in imaging HepG2 cells and zebrafish. The fluorescence response of IPPA for calf serum are powerful proofs for practical application. Therefore, IPPA has high potential for bioassay applications.
关键词： Fluorescent probe,Imaging,Cysteine,Cellular detection,Density functional theory calculations
更新于2025-09-19 17:15:36
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A novel coumarin-based fluorescent probe for sensitive detection of copper(II) in wine
摘要： A novel coumarin-based fluorescent probe (probe 1) for the detection of copper(II) was developed. The fluorescence intensity of probe 1 showed a linear relationship with the concentration of copper(II) in the range 0–16 μM (0–1.02 mg/L) and the limit of detection was 62 nM (3.94 μg/L). The luminescence of probe 1 at the maximum allowable amounts of copper(II) in wine and water could be observed with the naked eye under a 365-nm ultraviolet lamp. Moreover, probe 1 was successfully used for the qualitative and quantitative detection of copper(II) in wine.
关键词： fluorescent probe,red wine,Copper(II)
更新于2025-09-19 17:15:36