研究目的
To develop a long-wavelength-emitting fluorescent probe for simultaneous discrimination of H2S/Cys/GSH and its application in bio-imaging.
研究成果
The probe NR-CY is a highly sensitive and selective long-wavelength-emitting fluorescent probe for the simultaneous detection of H2S, Cys, and GSH. It exhibits rapid response times and has been successfully applied for live cell imaging, demonstrating its potential for biological applications.
研究不足
The probe's performance in purely aqueous solutions may be limited due to its poor solubility in water. The study primarily focuses on in vitro applications, and further in vivo studies are needed to fully assess its bio-imaging capabilities.
1：Experimental Design and Method Selection:
The probe NR-CY was synthesized by combining the derivative of Nile red with 7-nitrobenzofurazan. The response mechanisms to thiols are regulated by intramolecular charge transfer (ICT) and photoinduced electron transfer (PET) mechanisms.
2：Sample Selection and Data Sources:
The probe's sensitivity and selectivity were tested against various ions and small molecules in acetonitrile/PBS buffer system.
3：List of Experimental Equipment and Materials:
UV-2102 dual beam UV-Vis spectrometer, F-4500 FL spectrophotometer, Bruker DTX-400 spectrometer, HPLC-Q-HR/MS spectrometer, Nikon-80i inverted fluorescence microscope.
4：Experimental Procedures and Operational Workflow:
The probe's absorption and fluorescent properties were measured in different solvents. The selectivity and sensitivity were examined by comparing the fluorescence intensity of the product obtained by the probe reacting with various ions and small molecules.
5：Data Analysis Methods:
The fluorescence intensity was analyzed to determine the probe's response to thiols.
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