- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
Sinergic Effect of Therapeutic Ultrasound and Low-Level Laser Therapy in the Treatment of Hands and Knees Ostheoarthritis
摘要： Osteoarthritis is a degenerative joint disease that affects predominantly hands and knees of the elderly population, being characterized by chronic pain and limitation of joint movements. Therapeutic approaches to ease the pain, as low-intensity pulsed ultrasound and photobiomodulation (low-level laser therapy) have been broadly used as a complement to drug treatment of osteoarthritis. The aim of this study was to evaluate the synergic effect of LIPUS associated to LLLT on osteoarthritis of hands and knees. For this, 69 patients, being 48 affected by knee osteoarthritis and 21 affected by hand osteoarthritis were selected for the study. Patients were evaluated by the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) for knee osteoarthritis and by Australian Canadian Osteoarthritis Hand (AUSCAN), for hands osteoarthritis. All patients filled out the Visual Analogue Scale (VAS) pain questionnaire, which showed statistical improvement of hands (p<0.001) and knees (p<0.001) when comparing before and after treatment. Functional evaluation by AUSCAN showed improvement of hands functionality (p<0.002). Results showed that the synergic therapy of LIPUS and LLLT were efficient in the treatment of hands and knees osteoarthritis, providing a new approach of a non-pharmacological and non-invasive treatment that contributes to better quality of life for the patients with this chronic and degenerative pathology.
关键词： WOMAC,Low-level laser therapy,Osteoarthritis,Therapeutic ultrasound,AUSCAN
更新于2025-09-11 14:15:04
-
Efficacy of High-Intensity Laser Therapy and Silicone Insole in Plantar Fasciitis
摘要： Plantar fasciitis (PF), also known as plantar heel pain syndrome, heel spur syndrome, or painful heel syndrome, is an inflammatory disease of the plantar fascia. Many different methods have been used to treat it, but optimal treatment for this clinical condition is still unknown. 52 patients treated with (High-intensity laser therapy) HILT + insole and only insole was included in the study retrospectively. They had been assessed for the pain in the first steps in the morning, after 10 minute and 60 minute walk with 10 cm visual analogue scale (VAS) and Heel Tenderness Index (HTI), Foot and Ankle Outcome Score (FAOS), and measurement of plantar fascia thickness. Both groups showed a significant improvement regarding all parameters (pain scores, function and quality of life scores, and fat pad thickness) one month after treatment. When the pre and post-treatment percentage changes were compared, a significant difference was found between both groups regarding all VAS score parameters, FAOS foot and ankle related quality of life, FAOS pain, and HTI scores in HILT and insole group. HILT + insole therapy was considered to be more effective than only silicone insole with regard to decrease in pain and increase in quality of life.
关键词： High-intensity laser therapy,Insole,Plantar fasciitis
更新于2025-09-11 14:15:04
-
LOW-LEVEL LIGHT-ASSISTED PHOTODYNAMIC THERAPY USING A WEARABLE CAP-LIKE DEVICE FOR THE TREATMENT OF ACTINIC KERATOSIS OF THE SCALP
摘要： Background: Daylight photodynamic therapy (dlPDT) is a painless and increasingly cost-effective treatment for actinic keratosis (AK). New protocols avoid incubation minimizing pain and adverse events. However, it is time-consuming and dependent on specific weather conditions. In patients with AK of the scalp, we evaluated the efficacy of indoor photodynamic therapy (PDT) using a wearable low-level light therapy (LLLT) device, without pre-incubation with a photosensitizing agent. Methods: In this pilot study, 27 patients with thin and moderately thick AK (Olsen Grades I-II) underwent a single 15-minute session of LLLT using a wearable cap-like device immediately after application of methyl-aminolevulinate (MAL) cream, with no prior preparation of the affected area. Treatment efficacy was quantified by measuring the reduction in AK lesion number and the AK quality of life (AKQoL) score. All AK lesions were mapped at baseline for follow-up 2 months later. Paired pre/post scalp biopsies from 5 patients were analysed using histological and immunohistochemical techniques (p53, p27, cyclin D1, p63, and Ki67 expression). Data were analysed using the Wilcoxon signed-rank test. Results: In all patients we observed a global reduction in the number of AK lesions (71%; p<0.0001) and AKQoL score (from 5.6 to 4.4; p=0.034) 2 months after treatment. Histology and immunohistochemistry of skin biopsies from 5 patients also revealed marked improvements after LLLT. No patients reported any pain during treatment. Conclusion: PDT using LLLT is a rapid, painless, and efficacious modality for the treatment of AK.
关键词： helmet,skin cancer,low-level laser therapy,Actinic keratosis,photodynamic therapy,methylaminolevulinate
更新于2025-09-10 09:29:36
-
Photobiomodulation for the treatment of retinal diseases: a review
摘要： Photobiomodulation (PBM), also known as low level laser therapy, has recently risen to the attention of the ophthalmology community as a promising new approach to treat a variety of retinal conditions including age-related macular degeneration, retinopathy of prematurity, diabetic retinopathy, Leber's hereditary optic neuropathy, amblyopia, methanol-induced retinal damage, and possibly others. This review evaluates the existing research pertaining to PBM applications in the retina, with a focus on the mechanisms of action and clinical outcomes. All available literature until April 2015 was reviewed using PubMed and the following keywords: 'photobiomodulation AND retina', 'low level light therapy AND retina', 'low level laser therapy AND retina', and 'FR/NIR therapy AND retina'. In addition, the relevant references listed within the papers identified through PubMed were incorporated. The literature supports the conclusion that the low-cost and non-invasive nature of PBM, coupled with the first promising clinical reports and the numerous preclinical-studies in animal models, make PBM well-poised to become an important player in the treatment of a wide range of retinal disorders. Nevertheless, large-scale clinical trials will be necessary to establish the PBM therapeutic ranges for the various retinal diseases, as well as to gain a deeper understanding of its mechanisms of action.
关键词： low level laser therapy,age-related macular degeneration,retinopathy of prematurity,photobiomodulation,retinal degeneration,amblyopia,far-red to near-infrared,methanol toxicity,retinitis pigmentosa
更新于2025-09-10 09:29:36
-
The Possible Limitation of the Intra-Operative PDT Dosimetry: On Wounds Healing, Following Tumor Resection
摘要： In the past three decades, laser research in Saudi Arabia had provided methodical findings for the advancement of laser application in medicine. At low power emission, lasers incite photochemical effects, which diverge into two inevitable therapeutic phenomena Photo Dynamic Therapy (PDT) and Low Power Laser Therapy (LPLT). The term PDT refers to the combined use of a Photo Sensitizer (PS) (Dye) plus Laser to treat and diagnose malignant cells. In the doses usually used for treatments, the dye alone or the laser alone has no effect. PDT, a laser treatment for cancer, involves light and PS, (i.e. a light-sensitive drug, for instance – Hemato Porphyrin Derivative (HPD), Photofrin, and 5-Aminolevulinic Acid etc.) for the selective destruction of Neoplastic cells. Rigorous PDT studies in our laboratory are ongoing since 1985 aiming to determine the most suitable laser wavelength and PS, in thyroid tumor using nude mice as animal models. Previously we have established the Clonogenic Survival Rates, and tumor growths following PDT as a function of tumor volume for RIF-1 (murine fibro sarcoma) in C3H mice. We were able to completely eradicate small tumors (i.e. tumor volume < 50 mm3), and decrease the growth rates of larger tumors by executing superficial PDT followed by interstitial PDT. Thus, unlike ionizing radiation, PDT may be repeated if necessary without yielding cumulative toxicity [1-4]. The high laser doses required for the presents widely used dye {Photofrin @ I and II (HPD)}, may result, as our recent experiments show, in inhibiting Wound Healing of up to 8%. Where if the PDT laser dose decreased it could stimulate acceleration of wound healing up to 30%.Where the Incident dose for PDT with Photofrin of 80 J/cm2 give zero bio activation for wound healing. With regard to LPLT, we study its effects on wounds and burns in Sprague-Dawley rats. We noticed healing acceleration, zero-bio activation, and inhibition that depended on the wavelength, dose and treatment schedule, but not on dose rate and laser-skin transmission. We further observed that LPLT had higher wound healing acceleration than Light Emitting Diode (LED), or pharmaceutical treatments. We found 29% healing acceleration with HeNe (632.8 nm) laser at 5 J/cm2 (human dose) 40 J/cm2 (animal dose), applied three times a week. 42% of the animals had better cosmos’s .When wound healing was impaired by diabetes, LPLT accelerated healing by +8.6% relative to diabetic controls (-42%) [5-8]. This support the suggestion of using other dyes (new PS, developed to overcome some of other problems, like unacceptable prolongation of the procedure if large area treated and poor absorption of light By HPD). Since then several classes of PS were developed. The main classes are: porphyrin derivatives, Chlorins, Phthalocyanines and Porphycenes etc., Must have the properties of high absorption of laser, and deep tissue penetrations. Also these dyes should use a simulative laser dose for the wound healing, as the PDT optimum dose when combined with surgery, beside its efficacy for selectively destroying malignant tissue. The laser incident dose For PDT suggested from our experiments is up to 40 J/cm2 to give also wound healing acceleration in the animal, (which should converted to human),and up to 140 J/cm2 for deceleration in the animal. We advocate the Intraoperative Photo Dynamic Therapy (IPDT). Initially, surgical lasers excise/ablate visible tumors sealing the lymph vessels in the process thereby preventing further metastasis. Highly selective PDT follows removing microscopic residual cancer cells that remain after the surgery. Finally, LPLT is applied to accelerate healing of postoperative normal wounds (and /or Diabetic wounds). In time, medical lasers shall supersede some conventional therapies simply because of being overwhelmingly effective in various clinical applications.
关键词： Stimulation,HPD,Photo-bio modulation,Photo dynamic therapy,Low power laser therapy,Tumors,Photo sensitizer,Inhibition,Wound healing
更新于2025-09-04 15:30:14
-
Improvement in infected wound healing in type 1 diabetic rat by the synergistic effect of photobiomodulation therapy and conditioned medium
摘要： We investigated the effects of photobiomodulation therapy (PBMT) and conditioned medium (CM) of human bone marrow mesenchymal stem cells (hBM‐MSC) individually and/or in combination on the stereological parameters and the expression of basic fibroblast growth factor (bFGF), hypoxia‐inducible factor (HIF‐1α), and stromal cell–derived factor‐1α (SDF‐1α) in a wound model infected with methicillin‐resistant Staphylococcus aureus (MRSA) in diabetic rats. CM was provided by culturing hBM‐MSCs. Type 1 diabetes mellitus (T1DM) was induced in 72 rats, divided into four groups, harboring 18 rats each: group 1 served as a control group, group 2 received PBMT, group 3 received CM, and group 4 received CM + PBMT. On days 4, 7, and 15, six animals from each group were euthanized and the skin samples were separated for stereology examination and gene expression analysis by real‐time polymerase chain reaction. In the CM + PBMT, CM, and PBMT groups, significant decreases were induced in the number of neutrophils (1460 ± 93, 1854 ± 138, 1719 ± 248) and macrophages (539 ± 69, 804 ± 63, 912 ± 41), and significant increases in the number of fibroblasts (1073 ± 116, 836 ± 75, 912 ± 41) and angiogenesis (15 230 ± 516, 13 318 ± 1116, 14 041 ± 867), compared with those of the control group (2690 ± 371, 1139 ± 145, 566 ± 90, 12 585 ± 1219). Interestingly, the findings of the stereological examination in the CM + PBMT group were statistically more significant than those in the other groups. In the PBMT group, in most cases, the expression of bFGF, HIF‐1α, and SDF‐1α, on day 4 (27.7 ± 0.14, 28.8 ± 0.52, 27.5 ± 0.54) and day 7 (26.8 ± 1.4, 29.6 ± 1.4, 28.3 ± 1.2) were more significant than those in the control (day 4, 19.3 ± 0.42, 25.5 ± 0.08, 22.6 ± 0.04; day 7, 22.3 ± 0.22, 28.3 ± 0.59, 24.3 ± 0.19) and other treatment groups. The application of PBMT + CM induced anti-inflammatory and angiogenic activities, and hastened wound healing process in a T1 DM model of MRSA infected wound.
关键词： stereology,diabetes mellitus,diabetic foot ulcer,methicillin‐resistant staphylococcal aureus,low‐level laser therapy,phobiomodulation therapy,human bone marrow mesenchymal stem cell‐conditioned medium,real‐time PCR,rat
更新于2025-09-04 15:30:14
-
Photobiomodulation at Multiple Wavelengths Differentially Modulates Oxidative Stress <i>In Vitro</i> and <i>In Vivo</i>
摘要： Photobiomodulation (PBM) is emerging as an effective strategy for the management of multiple inflammatory conditions, including oral mucositis (OM) in cancer patients who receive chemotherapy or radiotherapy. Still, the poor understanding of the mechanisms by which the light interacts with biological tissues and the heterogeneity of light sources and protocols employed worldwide significantly limits its applicability. Reactive oxygen species (ROS) are massively generated during the early phases of OM and play a major role in the pathogenesis of inflammation in general. Here, we report the results of a clinical and experimental study, aimed at evaluating the effect of laser light at different wavelengths on oxidative stress in vivo in oncologic patients suffering from OM and in vitro in two cell types abundantly present within the inflamed oral mucosa, neutrophil polymorphonuclear (PMN) granulocytes, and keratinocytes. In addition to standard ROS detection methods, we exploited a roGFP2-Orp1 genetically encoded sensor, allowing specific, quantitative, and dynamic imaging of redox events in living cells in response to oxidative stress and PBM. We found that the various wavelengths differentially modulate ROS production. In particular, the 660 nm laser light increases ROS production when applied either before or after an oxidative stimulus. In contrast, the 970 nm laser light exerted a moderate antioxidant activity both in the saliva of OM patients and in both cell types. The most marked reduction in the levels of ROS was detected in cells exposed either to the 800 nm laser light or to the combination of the three wavelengths. Overall, our study demonstrates that PBM exerts different effects on the redox state of both PMNs and keratinocytes depending on the used wavelength and prompts the validation of a multiwavelength protocol in the clinical settings.
关键词： Reactive oxygen species,Laser therapy,Photobiomodulation,Oxidative stress,Oral mucositis
更新于2025-09-04 15:30:14