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<p>A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells</p>
摘要： Quantum dots (QDs) are used as fluorescent probes due to their high fluorescence intensity, longevity of fluorescence, strong light-resistant bleaching ability and high light stability. Therefore, we explore a more precise probe that can target an organelle. Methods: In the current study, a new class of fluorescence probes were developed using QDs capped with 4 different L-cysteine-polyamine-morpholine linked by mercapto groups. Ligands were characterised by Electrospray ionization mass spectrometry (ESI-MS), H-Nuclear Magnetic Resonance (1H NMR) spectroscopy, and 13C NMR spectroscopy. Modified QDs were characterized by Transmission Electron Microscope (TEM), Ultraviolet and visible spectrophotometry (UV–Vis), and fluorescence microscopy. And the biological activity of modified QDs was explored by using MTT assay with HeLa, SMMC-7721 and HepG2 cells. The fluorescence imaging of modified QDs was obtained by confocal laser scanning fluorescence microscopy (CLSM). Results: Synthesized QDs ranged between 4 to 5 nm and had strong optical emission properties. UV–Vis and fluorescence spectra demonstrated that the cysteine-polyamine-morpholine were successfully incorporated into QD nanoparticles. The MTT results demonstrated that modified QDs had lesser cytotoxicity when compared to unmodified QDs. In addition, modified QDs had strong fluorescence intensity in HeLa cells and targeted lysosomes of HeLa cells. Conclusion: This study demonstrates the modified QDs efficiently entered cells and could be used as a potential lysosome-targeting fluorescent probe.
关键词： lysosome-targetable,cells imaging,quantum dots,fluorescence probe
更新于2025-09-23 15:19:57
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A lysosome-targetable fluorescent probe for simultaneously sensing Cys/Hcy, GSH, and H2S from different signal patterns
摘要： Biothiols, a vital branch of reactive sulfur species (RSS) family, which are indispensable in human physiology. However, the exact functional roles of each biothiols involved in complicated physiological activities were still not fully clarified. A critical barrier is a lack of robust molecular tools which can simultaneously visualize different biothiols with distinct emission signals. Herein, the first lysosome-targetable fluorescent probe, Lyso-RC, which could respond to Cys/Hcy, GSH and H2S with different sets of signal patterns was developed. Lyso-RC responds to Cys/Hcy, GSH and H2S with the fluorescence signal patterns of blue-red, green-red, and red, respectively. Significantly, Lyso-RC is capable of discriminating lysosomal Cys/Hcy, GSH, and H2S in HeLa cells.
关键词： imaging,lysosome-targetable,simultaneous detection,fluorescent probe,biothiols
更新于2025-09-10 09:29:36
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A smart approach toward rhodamine spiro-ring derivatives sensing platform for lysosome-targetable imaging applications
摘要： As acidic organelles (pH 4.0-5.5) in living cells, lysosomes play key roles in numerous cellular biological processes, and associate with diverse diseases. Small molecular probes based fluorescence imaging techniques capable of providing information on target analytes in lysosomes are highly appreciated. Herein, for the first time we propose a new strategy of constructing pH-independent rhodamine based spiro-ring platform for lysosomal imaging applications. As a proof of concept, by incorporating lysosome specific moiety morpholine, a thio-lactone rhodamine analogue HN4MS for HOCl with high sensitivity, high selectivity and fast response at lysosomal pH value was developed. Moreover, possessing ability of imaging in lysosome organelles, the new probe was successfully applied to visualize exogenous and endogenous HOCl in living cells and tissues, which verified the present avenue for expansion of organelle targetable imaging tools.
关键词： Spiro-ring,Imaging,Fluorescent probe,Lysosome-targetable,HOCl
更新于2025-09-09 09:28:46