研究目的
To develop a lysosome-targetable fluorescent probe for simultaneously sensing Cys/Hcy, GSH, and H2S from different signal patterns.
研究成果
Lyso-RC is the first lysosome-targetable fluorescent probe capable of simultaneously distinguishing lysosomal Cys/Hcy, GSH, and H2S with distinct sets of signal patterns, offering a robust molecular tool for studying the complex interplay networks of biothiols in lysosome.
研究不足
The probe's ability to detect Cys/Hcy/H2S under high background GSH coexist needs further validation.
1：Experimental Design and Method Selection:
The probe Lyso-RC was designed with a coumarin dye and resorufin as fluorophores, connected via an amide bond and an ether bond, and a morpholine moiety as the lysosome targeting group.
2：Sample Selection and Data Sources:
HeLa cells were used to test the probe's capability in living cells.
3：List of Experimental Equipment and Materials:
Fluorescence spectra were recorded to monitor the probe's response to biothiols.
4：Experimental Procedures and Operational Workflow:
Time-dependent absorption and fluorescence spectra of Lyso-RC in the presence of biothiols were investigated.
5：Data Analysis Methods:
Fluorescence intensity changes were analyzed to determine the probe's sensitivity and selectivity towards biothiols.
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