- 标题
- 摘要
- 关键词
- 实验方案
- 产品
-
NanoJ: a high-performance open-source super-resolution microscopy toolbox
摘要： Super-resolution microscopy has become essential for the study of nanoscale biological processes. This type of imaging often requires the use of specialised image analysis tools to process a large volume of recorded data and extract quantitative information. In recent years, our team has built an open-source image analysis framework for super-resolution microscopy designed to combine high performance and ease of use. We named it NanoJ - a reference to the popular ImageJ software it was developed for. In this paper, we highlight the current capabilities of NanoJ for several essential processing steps: spatio-temporal alignment of raw data (NanoJ-Core), super-resolution image reconstruction (NanoJ-SRRF), image quality assessment (NanoJSQUIRREL), structural modelling (NanoJ-VirusMapper) and control of the sample environment (NanoJ-Fluidics). We expect to expand NanoJ in the future through the development of new tools designed to improve quantitative data analysis and measure the reliability of fluorescent microscopy studies.
关键词： Virus,Vaccinia,Archaea,Quantitative imaging,Sulfolobus acidocaldarius,Super-resolution microscopy,Fluidics,Modelling,Resolution,Image quality assessment,Pox,Image analysis,ImageJ,Fiji
更新于2025-09-19 17:15:36
-
[IEEE 2018 Digital Image Computing: Techniques and Applications (DICTA) - Canberra, Australia (2018.12.10-2018.12.13)] 2018 Digital Image Computing: Techniques and Applications (DICTA) - Classifier-Free Extraction of Power Line Wires from Point Cloud Data
摘要： This paper proposes a classi?er-free method for extraction of power line wires from aerial point cloud data. It combines the advantages of both grid- and point-based processing of the input data. In addition to the non-ground point cloud data, the input to the proposed method includes the pylon locations, which are automatically extracted by a previous method. The proposed method ?rst counts the number of wires in a span between the two successive pylons using two masks: vertical and horizontal. Then, the initial wire segments are obtained and re?ned iteratively. Finally, the initial segments are extended on both ends and each individual wire points are modelled as a 3D polynomial curve. Experimental results show both the object-based completeness and correctness are 97%, while the point-based completeness and correctness are 99% and 88%, respectively.
关键词： power line,point cloud,extraction,wire,modelling
更新于2025-09-19 17:15:36
-
[IEEE 2018 IEEE SENSORS - New Delhi, India (2018.10.28-2018.10.31)] 2018 IEEE SENSORS - Rectangular Array Current Transducer with Integrated Microfluxgate Sensors
摘要： Novel rectangular yokeless current transducer with the range 400 A using 16 microfluxgate sensors around the busbar conductor is presented in this paper. Compared to yokeless transducers utilizing the differential pair of magnetic sensors, our solution has much better suppression of external currents (lower crosstalk). Compared to industrial transducers with yoke, the new transducer has 10-times lower noise, 10-times better temperature stability, and same crosstalk. Sensor design, different methods for calculating the current and temperature dependence are presented in this paper. Crosstalk error is examined in dependence on the number of the operating sensors and external current position.
关键词： Finite element modelling,current sensor,microfluxgate sensors,rectangular sensor array
更新于2025-09-19 17:15:36
-
[IEEE 2018 International Joint Conference on Neural Networks (IJCNN) - Rio de Janeiro (2018.7.8-2018.7.13)] 2018 International Joint Conference on Neural Networks (IJCNN) - Investigation into Sub-Receptive Fields of Retinal Ganglion Cells with Natural Images
摘要： Determining the receptive field of a retinal ganglion cell is critically important when formulating a computational model that maps the relationship between the stimulus and response. This process is traditionally undertaken using reverse correlation to estimate the receptive field. By stimulating the retina with artificial stimuli, such as alternating checkerboards, bars or gratings and recording the neural response it is possible to estimate the cell’s receptive field by analysing the stimuli that produced the response. Artificial stimuli such as white noise is known to not stimulate the full range of the cell’s responses. By using natural image stimuli, it is possible to estimate the receptive field and obtain a resulting model that more accurately mimics the cells’ responses to natural stimuli. This paper extends on previous work to seek further improvements in estimating a ganglion cell’s receptive field by considering that the receptive field can be divided into subunits. It is thought that these subunits may relate to receptive fields which are associated with bipolar retinal cells. The findings of this preliminary study show that by using subunits to define the receptive field we achieve a significant improvement over existing approaches when deriving computational models of the cell’s response.
关键词： computational modelling,visual neuroscience,receptive field,retinal ganglion cell
更新于2025-09-19 17:15:36
-
3D Printed Floating Photocatalysts for Wastewater Treatment
摘要： Organic contaminants, specifically contaminants of emerging concern (CECs), have a great environmental impact, since the removal of these pollutants is of great difficulty by conventional treatments and the presence of these pollutants in the aquatic medium, even at low concentrations, is extremely hazardous to human health. Advanced oxidation processes and, specifically, TiO2-photocatalytic process is considered an option with positive results for an efficient treatment. However, the photocatalyst must be accessible to the UV radiation, for the activation of the TiO2. For this reason, it is recommendable to use a floating photocatalyst (with lower density than water) if the UV light comes from the solar radiation, because it will be on the water surface. In addition, this characteristic of the catalyst can entail an increase of the process efficiency if the pollutant is mainly located on the surface of water. In this context, the goal of this work is the preparation of floating photocatalysts for the removal of CECs from wastewater. TiO2 is deposited in low-density-polyethylene (LDPE), support with lower density than water and high stability and resistance to degradation. LDPE-TiO2 mixtures were prepared by different methods: mixing TiO2 and LDPE in a hot-cylinder-mixer or using o-xylene or an anionic surfactant as dispersing agent, in order to increase the dispersion of TiO2 before extrusion. Filaments obtained were printed as meshes in a Fused-Deposition-Modelling 3D-printer. The printed photocatalysts improved the activity in comparison with the plate obtained in the cylinder, used as benchmark. Thus, this study opens the doors to the in-situ treatment of CECs, using floating photocatalysts and solar radiation as the sole reagent, a very economical, efficient, easily implantable and environmentally compatible process.
关键词： photocatalysis,polyethylene mesh,wastewater treatment,3D printing.,Fused Deposition Modelling
更新于2025-09-19 17:15:36
-
Modelling and Terrestrial Laser Scanning Methodology (2009a??2018) on Debris Cones in Temperate High Mountains
摘要： Debris cones are a very common landform in temperate high mountains. They are the most representative examples of the periglacial and nival processes. This work studies the dynamic behavior of two debris cones (Cone A and Cone B) in the Picos de Europa, in the north of the Iberian Peninsula. Their evolution was measured uninterruptedly throughout each August for 10 years (2009–2018) using the Terrestrial Laser Scanning (TLS) technique. The observations and calculations of the two debris cones were treated independently, but both showed the same behavior. Therefore, if these results are extrapolated to other debris cones in similar environments (temperate high mountain), they should show behavior similar to that of the two debris cones analyzed. Material falls onto the cones from the walls, and transfer of sediments follows linear trajectories according to the maximum slope. In order to understand the linear evolution of the two debris cones, pro?les were created along the maximum slope lines of the Digital Elevation Model (DEM) of 2009, and these pro?le lines were extrapolated to the remaining years of measurement. In order to determine volumetric surface behavior in the DEMs, each year for the period 2009–2018 was compared. In addition, the statistical predictive value for position (Z) in year 2018 was calculated for the same planimetric position (X,Y) throughout the pro?les of maximum slopes. To do so, the real ?eld data from 2009–2017 were interpolated and used to form a sample of curves. These curves are interpreted as the realization of a functional random variable that can be predicted using statistical techniques. The predictive curve obtained was compared with the 2018 ?eld data. The results of both coordinates (Z), the real ?eld data, and the statistical data are coherent within the margin of error of the data collection.
关键词： mathematical modelling,surface dynamic,debris cones,terrestrial laser scanning,Picos de Europa
更新于2025-09-19 17:13:59
-
N-glycosylation state of TRPM8 protein revealed by terahertz spectroscopy and molecular modelling
摘要： TRPM8 member of the TRP superfamily of membrane proteins participates to various cellular processes ranging from Ca2+ uptake and cold sensation to cellular proliferation and migration. TRPM8 is a large tetrameric protein with more than 70% of its residues located in the cytoplasm. TRPM8 is N-glycosylated, with a single site per subunit. This work focuses on the N-glycosylation of TRPM8 channel that was previously studied by our group in relation to proliferation and migration of tumoral cells. Here, experimental data performed with deglycosylating agents assess that the sole glycosylation site contains complex glycans with a molecular weight of 2.5 kDa. The glycosylation state of TRPM8 in cells untreated and treated with a deglycosylating agent was addressed with Terahertz (THz) spectroscopy. Results show a clear difference between cells comprising glycosylated and deglycosylated TRPM8, the first presenting an increased THz absorption. Human TRPM8 was modelled using as templates the available TRPM8 and other TRPM channels structures. Glycosylations were modelled by considering two glycan structures with molecular weight close to the experiment: shorter and branched at the first sugar unit (glc1) and longer and unbranched (glc2). Simulation of THz spectra based on the molecular dynamics of unglycosylated and the two glycosylated TRPM8 models in lipid membrane and solvation box showed that glycan structure strongly influences the THz spectrum of the channel and of other components from the simulation system. Only spectra of TRPM8 with glc1 glycans were in agreement with the experiment, leading to the validation of glc1 glycan structure.
关键词： N-glycosylation,Terahertz spectroscopy,TRPM8,Molecular modelling,Membrane proteins
更新于2025-09-19 17:13:59
-
Reduction of electrical power loss in a photovoltaic chain in conditions of partial shading
摘要： The paper presents the problem of losses of electrical power in photovoltaic chains during partial shading. Photovoltaic modules work best when the illumination is uniform over the entire light-sensitive surface. If a part of this surface is illuminated to a lesser extent, it often ceases to be a part of the electricity generator, but constitutes a resistance. To minimize this phenomenon, PV modules use bypass diodes, e.g. covering several dozen cells in a photovoltaic chain. In addition, parts of the photovoltaic module surface with lower exposure to sunlight may be additionally illuminated with a mirror positioned to reflect light. The analysis uses a photovoltaic chain model implemented in MATLAB/Simulink. The model describes the processes of electrical power generation in photovoltaic modules. The model corresponds to a laboratory photovoltaic system located in AGH in Cracow. In the presented model, each individual part may be illuminated with different light intensity. The light incident on the cells is a direct sunlight or light reflected from the mirror. The model allows for the analysis of the electrical power generated depending on the light profile of the photovoltaic system. The results of the model analysis may be applied in optimization of the PV modules’ configuration.
关键词： Partial shading,Modelling,Photovoltaic system,Sunlight reflection,Maximum power point tracking (MPPT)
更新于2025-09-19 17:13:59
-
Performance assessment of a hybrid photovoltaic-thermal and heat pump system for solar heating and electricity
摘要： This work investigates a solar combined heat and power systems based on hybrid photovoltaic-thermal heat pump systems for the simultaneous provision of space heating and electricity to residential homes. The analysed system connects a photovoltaic-thermal (PVT) panel, through a PVT water tank, to a heat pump. The study is based on quasi-steady state heat transfer and thermodynamic analysis that takes incremental time steps to solve for the fluids temperature changes from the heat pump and the solar PVT panels. The effects of solar irradiance, size of the water tank and the water flow rate in the PVT pipes (laminar and turbulent) on the performance of the system are analysed. Particular focus is made towards the efficiency (electrical and thermal) of the PVT and the COP of the heat pump. Results show that the minimum COP of the heat pump is 4.2, showing the high performance of the proposed hybrid system. Increasing the water flowrate through the PVT panel from 3L/min (laminar) to 17L/min (turbulent) increases the PVT’s total efficiency (electrical + thermal) from 61% to 64.5%. Increasing the size of the PVT water tank from 1L to 100L, increases the total efficiency of the PVT panel by 6.5%.
关键词： quasi-steady state modelling,heat pump,solar photovoltaic-thermal,hybrid system
更新于2025-09-19 17:13:59
-
Deciphering the role of key defects in Sb2Se3, a promising candidate for chalcogenide based solar cells
摘要： Herein we report a thorough investigation on Sb2Se3, a promising absorber material for photovoltaic applications, using state of the art quantum methods to understand the impact of defects on its electrical properties. The results show that despite a rather small stability domain, Sb2Se3 is easy to synthetize because there is no other possible stable competing binary phase in the Sb/Se system. Our calculations prove that formation of intrinsic n-type defects is unlikely, because Sb vacancies restrain the Fermi level from reaching the CBM vicinity. In contrast, intrinsic p-type semiconductor behavior is expected due to SbSe antisite defects. Doping is a commonly used technique to impact on the charge carrier concentration as well as the charge carrier nature. In that context, several extrinsic defects were considered, based on tin and copper to enhance the native p-typeness, and halogenides (Cl, Br, I) to induce n-type doping in Sb2Se3. Our results tend to prove that Sb2Se3:Cu(p)/Sb2Se3:I(n) might be a viable homojunction for photovoltaic devices.
关键词： point defects,chalcogenides,absorber,photovoltaics,Sb2Se3,modelling,DFT
更新于2025-09-19 17:13:59