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Analysis and Design of High Frequency 1-D CMUT Imaging Arrays in Non-collapsed Mode
摘要： High frequency ultrasound imaging arrays are important for a broad range of applications, from small animal imaging to photoacoustics. CMUT arrays are particularly attractive for these applications as low noise receiver electronics can be integrated for an overall improved performance. In this paper we present a comprehensive analysis of high frequency CMUT arrays based on an experimentally verified CMUT array simulation tool. The results obtained on an example, a 40 MHz 1-D CMUT array for intravascular imaging, are used to obtain key design insights and tradeoffs for receive only and pulse-echo imaging. For the receiver side, thermal mechanical current noise, plane wave pressure sensitivity, and pressure noise spectrum are extracted from simulations. Using these parameters, we find that the receiver performance of CMUT arrays can be close to an ideal piston, independent of gap thickness and applied DC bias, when coupled to low noise electronics with arrays utilizing smaller membranes performing better. For pulse-echo imaging, thermal mechanical current noise limited SNR is observed to be dependent on the maximum available voltage and gap thickness. In terms of bandwidth, we find that Bragg resonance of the array, related to the fill factor, is a significant determinant of the high frequency limit and the fluid loaded single membrane resonance determines the lower limit. Based on these results, we present design guidelines requiring only fluid loaded single membrane simulations and membrane pitch to achieve a desired pulse-echo response. We also provide a design example and discuss limitations of the approach.
关键词： CMUT,Bragg’s resonance,CMUT design,High frequency arrays,Phased array,Photoacoustic receiver
更新于2025-09-04 15:30:14
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Measurement of the thermal expansion coefficient of Guadua angustifolia-Kunth using the photoacoustic technique
摘要： In this paper, the Linear Thermal Expansion Coefficient of Guadua angustifolia–Kunth samples was measured using the Photoacoustic (PA) technique in a heat transmission configuration and considering the thermoelastic bending as a PA signal generation mechanism in addition to the thermodiffusion ones. The obtained value of (27±7)×10-6K-1 is a reasonable value compared with that reported for similar materials such as wood.
关键词： photoacoustic technique,thermal expansion coefficient,Guadua angustifolia-Kunth
更新于2025-09-04 15:30:14
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Evaluation of renal oxygen saturation using photoacoustic imaging for the early prediction of chronic renal function in a model of ischemia-induced acute kidney injury
摘要： To evaluate the utility of photoacoustic imaging in measuring changes in renal oxygen saturation after ischemia-induced acute kidney injury, and to compare these measurements with histological findings and serum levels of kidney function.
关键词： chronic renal function,ischemia-reperfusion,renal oxygen saturation,acute kidney injury,photoacoustic imaging
更新于2025-09-04 15:30:14
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Gadolinium Doping Enhances the Photoacoustic Signal of Synthetic Melanin Nanoparticles: A Dual Modality Contrast Agent for Stem Cell Imaging.
摘要： In this paper we show that gadolinium-loaded synthetic melanin nanoparticles (Gd(III)-SMNPs) exhibit up to a 40-fold enhanced photoacoustic signal intensity relative to synthetic melanin alone and higher than other metal-chelated SMNPs. This property makes these materials useful as dual labelling agents because Gd(III)-SMNPs also behave as magnetic resonance imaging (MRI) contrast agents. As a proof-of-concept, we used these nanoparticles to label human mesenchymal stem cells (hMSCs). Cellular uptake was confirmed with bright field optical and transmission electron microscopy. The Gd(III)-SMNP labeled stem cells continued to express the stem cell surface markers CD73, CD90, and CD105 and proliferate. The labeled stem cells were subsequently injected intramyocardially in mice, and the tissue was observed by photoacoustic and MR imaging. We found the photoacoustic signal increased as cell number increased (R2 = 0.96) indicating that such an approach could be employed to discriminate between stem cells populations with a limit of detection of 2.3 x 104 cells in in vitro tests. This multimodal photoacoustic/MRI approach combines the excellent temporal resolution of photoacoustics with the anatomic resolution of MRI.
关键词： Photoacoustic Signal,Stem Cell Imaging,Gadolinium,Dual Modality Contrast Agent,Synthetic Melanin Nanoparticles
更新于2025-09-04 15:30:14
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Exosome-like Nanozyme Vesicles for H2O2-responsive Catalytic Photoacoustic Imaging of Xenograft Nasopharyngeal Carcinoma
摘要： Photoacoustic imaging (PAI) is an attractive imaging modality, which is promising for clinical cancer diagnosis due to its advantages on deep tissue penetration and fine spatial resolution. However, few tumor catalytic/responsive PAI strategies are developed. Here, we design an exosome-like nanozyme vesicle for in vivo H2O2-responsive PAI of nasopharyngeal carcinoma (NPC). The intrinsic peroxidase-like activity of graphene quantum dot nanozyme (GQDzyme) effectively converts the 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) into its oxidized form in the presence of H2O2. The oxidized ABTS exhibits strong near-infrared (NIR) absorbance, rendering it to be an ideal contrast agent for PAI. Thus, GQDzyme/ABTS nanoparticle is a novel type of catalytic PAI contrast agent which is sensitive to H2O2 produced from NPC cells. Furthermore, we develop an approach to construct exosome-like nanozyme vesicle via biomimetic functionalization of GQDzyme/ABTS nanoparticle with natural erythrocyte membrane modified with folate acid. In vivo animal experiments demonstrated that, this exosome-like nanozyme vesicle effectively accumulated in NPC and selectively triggered catalytic PAI for NPC. In addition, our nanozyme vesicle exhibits excellent biocompatibility and stealth ability for long blood circulation. Together, we demonstrate that GQDzyme/ABTS based exosome-like nanozyme vesicle is an ideal nanoplatform for developing deep-tissue tumor-targeted catalytic PAI in vivo.
关键词： photoacoustic imaging,nasopharyngeal carcinoma,H2O2-responsive,graphene quantum dot nanozyme,exosome-like vesicle,erythrocyte membranes
更新于2025-09-04 15:30:14
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Dual Path Lock-In System for Elimination of Residual Amplitude Modulation and SNR Enhancement in Photoacoustic Spectroscopy
摘要： A technique for elimination of residual amplitude modulation (ERAM) in photoacoustic spectroscopy based on dual path lock-in was proposed and experimentally demonstrated. There are two lock-in amplifiers, one is for gas concentration demodulation and another for residual amplitude modulation (RAM) measurement by tuning the reference signal in different phases, and then a dual path lock-in technique based on subtraction is applied to RAM removal, improving the second harmonic profile significantly. In this system, the signal to noise ratio (SNR) increases about two times based on our dual path lock-in technique compared to one distributed feedback laser diode (DFB-LD). The system achieved a good linear response (R-square = 0.99887) in a concentration range from 100 ppmv to 2400 ppmv and a minimum detection limit (MDL) of 1.47 ppmv.
关键词： photoacoustic spectroscopy,RAM,dual path lock-in
更新于2025-09-04 15:30:14
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Auto-fluorescent polymer nanotheranostics for self-monitoring of cancer therapy via triple-collaborative strategy
摘要： Aberrant regulation of angiogenesis supply sufficient oxygen and nutrients to exacerbate tumor progression and metastasis. Taking this hallmark of cancer into account, reported here is a self-monitoring and triple-collaborative therapy system by auto-fluorescent polymer nanotheranostics which could be concurrently against angiogenesis and tumor cell growth by combining the benefits of anti-angiogenesis, RNA interfere and photothermal therapy (PTT). Auto-fluorescent amphiphilic polymer polyethyleneimine-polylactide (PEI-PLA) with positive charge can simultaneously load hydrophobic antiangiogenesis agent combretastatin A4 (CA4), NIR dye IR825 and absorb negatively charged heat shock protein 70 (HSP70) inhibitor (siRNA against HSP70) to construct self-monitoring nanotheranostics (NPICS). NPICS can effectively restrain the expression of HSP70 to reduce their endurance to the IR825-mediated PTT, leading to an enhanced photocytotoxicity. In a xenograft mouse tumor model, NPICS show an effect of inhibition of tumor angiogenesis and also display a highly synergistic anticancer efficacy with NIR laser irradiation. Significantly, based on its inherent auto-fluorescence, PEI-PLA not only serves as the drug carrier, but also as the self-monitor to real-time track NPICS biodistribution and tumor accumulation via fluorescence imaging. Moreover, IR825 endows NPICS could also be used as photoacoustic (PA) agents for in vivo PA imaging. This nanoplatform shows enormous potentials in cancer theranostics.
关键词： photothermal therapy,gene silencing,self-monitoring,fluorescence/photoacoustic imaging,anti-angiogenesis therapy
更新于2025-09-04 15:30:14
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Quantitative Evaluation of Broadband Photoacoustic Spectroscopy in the Infrared with an Optical Parametric Oscillator
摘要： We evaluate the spectral resolution and the detection thresholds achievable for a photoacoustic spectroscopy (PAS) system in the broadband infrared wavelength region 3270 nm to 3530 nm driven by a continuous wave optical parametric oscillator (OPO) with P ≈ 1.26 W. The absorption spectra, IPAS(λi), for diluted propane, ethane and methane test gases at low concentrations (c ～ 100 ppm) were measured for ～1350 discrete wavelengths λi. The IPAS(λi) spectra were then compared to the high resolution cross section data, σFTIR, obtained by Fourier Transform Infrared Spectroscopy published in the HITRAN database. Deviations of 7.1(6)% for propane, 8.7(11)% for ethane and 15.0(14)% for methane with regard to the average uncertainty between IPAS(λi) and the expected reference values based on σFTIR were recorded. The characteristic absorption wavelengths λres can be resolved with an average resolution of δλres ～ 0.08 nm. Detection limits range between 7.1 ppb (ethane) to 13.6 ppb (methane). In an additional step, EUREQA, an artificial intelligence (AI) program, was successfully applied to deconvolute simulated PAS spectra of mixed gas samples at low limits of detection. The results justify a further development of PAS technology to support e.g., biomedical research.
关键词： photoacoustic spectroscopy,optical-parametric oscillator,PAS,gas sampling,hydrocarbons,spectral deconvolution,EUREQA,OPO
更新于2025-09-04 15:30:14