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Viscosity Measurements Using a Two-photon Ratiometric Fluorescent Sensor with Two Rotors
摘要： A new ratiometric two-photon fluorescence sensor, Qca-Cy2, comprised of a hybrid cyanine–carbazole platform with two rotating sites is described. Qca-Cy2 can report on mitochondrial viscosity within live cells, as well as in living tissues at depths of 50-130 μm by ratiometric fluorescence imaging using two-photon microscopy.
关键词： ratiometric,viscosity measurements,fluorescent sensor,mitochondrial viscosity,two-photon
更新于2025-09-23 15:21:01
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[Institution of Engineering and Technology 20th Italian National Conference on Photonic Technologies (Fotonica 2018) - Lecce, Italy (23-25 May 2018)] 20th Italian National Conference on Photonic Technologies (Fotonica 2018) - Detecting the influence of water vapour on the measurements of minor chemical gases with the Differential Absorption LIDAR technique
摘要： The monitoring of pollution is fundamental to guarantee the health and the safety of the environment and humans. In fact, pollution is strictly correlated to climate changes, acid rains and in disease occurrences. The Differential Absorption LIDAR (DIAL) is a remote sensing technique able to identify the chemical substances in the atmosphere. This method is able to give information along long distances (also larger than 1 km) and it is possible to provide the system with equatorial mounts, in order to extract areal or volumetric concentration field. In this work, the authors focus on the interaction of water vapour with minor chemical gas measurements. In fact, the DIAL equation is achieved assuming some hypothesis that could not be acceptable in any conditions. In the present paper is evaluated how water vapour could change the differential extinction coefficients and when influences the final measurements. Both analytical investigation and experimental measurement are provided. These methodologies have been applied to six pollutants: ammonia (NH3), Benzene (C6H6), Nitrogen Dioxide (NO2), Nitrous Oxide (N2O), Ozone (O3) and Sulphur Dioxide (SO2).
关键词： DIAL,LIDAR,Water Vapour,Minor Chemicals,Measurements
更新于2025-09-23 15:21:01
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Online dimensional control of rolled steel profiles using projected fringes
摘要： Fringe projection is a versatile method for mapping the topography of surfaces. In this paper, it is used to measure the defects on the head of railroad rails while the rails are moving. Railroad rails are made by hot rolling. The quality of the finished product is generally good, but surface texture will deteriorate with increasing temperature. A method for online inspection therefore is very desirable. In the present experiment, dimensional inspection of the railroad rails was made online while moving at a speed of 1–2 m/s. Therefore, it is important to minimize the registration time. To achieve this, we apply a method of fringe location with sub-pixel accuracy that requires only a single exposure for each registration.
关键词： Fringe projection,Railroad rails,Optical measurements
更新于2025-09-23 15:21:01
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Wide-Angle, Ultra-Wideband ISAR Imaging of Vehicles and Drones
摘要： In-situ, wide-angle, and ultra-wideband inverse synthetic aperture radar (ISAR) imaging of vehicles and drones is demonstrated using a portable ultra-wideband radar. In order to form well-focused ISAR images, motion compensation is performed before applying the k-space imaging algorithm. While the same basic motion compensation methodology is applied to both types of targets, a more complex motion model is needed to better capture the ?ight path of the drone. The resulting ISAR images clearly show the geometrical outline of the targets and highlight locations of prominent backscattering. The ISAR images are also assessed against images generated through instrumented targets or laboratory measurements, and the image quality is shown to be comparable.
关键词： ultra-wideband,radar imaging,vehicles,radar measurements,drones,inverse synthetic aperture radar
更新于2025-09-23 15:21:01
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Probing Cell Mechanics with Bead-Free Optical Tweezers in the <em>Drosophila</em> Embryo
摘要： Morphogenesis requires coordination between genetic patterning and mechanical forces to robustly shape the cells and tissues. Hence, a challenge to understand morphogenetic processes is to directly measure cellular forces and mechanical properties in vivo during embryogenesis. Here, we present a setup of optical tweezers coupled to a light sheet microscope, which allows to directly apply forces on cell-cell contacts of the early Drosophila embryo, while imaging at a speed of several frames per second. This technique has the advantage that it does not require the injection of beads into the embryo, usually used as intermediate probes on which optical forces are exerted. We detail step by step the implementation of the setup, and propose tools to extract mechanical information from the experiments. By monitoring the displacements of cell-cell contacts in real time, one can perform tension measurements and investigate cell contacts' rheology.
关键词： Drosophila embryo,Developmental Biology,in vivo imaging,optical tweezers,Light sheet microscopy,force measurements,Issue 141,cell mechanics
更新于2025-09-23 15:21:01
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Massively parallel coherent laser ranging using a soliton microcomb
摘要： Coherent ranging, also known as frequency-modulated continuous-wave (FMCW) laser-based light detection and ranging (lidar)1 is used for long-range three-dimensional distance and velocimetry in autonomous driving2,3. FMCW lidar maps distance to frequency4,5 using frequency-chirped waveforms and simultaneously measures the Doppler shift of the reflected laser light, similar to sonar or radar6,7 and coherent detection prevents interference from sunlight and other lidar systems. However, coherent ranging has a lower acquisition speed and requires precisely chirped8 and highly coherent5 laser sources, hindering widespread use of the lidar system and impeding parallelization, compared to modern time-of-flight ranging systems that use arrays of individual lasers. Here we demonstrate a massively parallel coherent lidar scheme using an ultra-low-loss photonic chip-based soliton microcomb9. By fast chirping of the pump laser in the soliton existence range10 of a microcomb with amplitudes of up to several gigahertz and a sweep rate of up to ten megahertz, a rapid frequency change occurs in the underlying carrier waveform of the soliton pulse stream, but the pulse-to-pulse repetition rate of the soliton pulse stream is retained. As a result, the chirp from a single narrow-linewidth pump laser is transferred to all spectral comb teeth of the soliton at once, thus enabling parallelism in the FMCW lidar. Using this approach we generate 30 distinct channels, demonstrating both parallel distance and velocity measurements at an equivalent rate of three megapixels per second, with the potential to improve sampling rates beyond 150 megapixels per second and to increase the image refresh rate of the FMCW lidar by up to two orders of magnitude without deterioration of eye safety. This approach, when combined with photonic phase arrays11 based on nanophotonic gratings12, provides a technological basis for compact, massively parallel and ultrahigh-frame-rate coherent lidar systems.
关键词： photonic chip,FMCW lidar,soliton microcomb,parallel distance and velocity measurements,coherent ranging
更新于2025-09-23 15:21:01
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Kinetics of graphitization of thin diamond-like carbon (DLC) films catalyzed by transition metal
摘要： In this paper, we have studied the kinetics of graphitization at 773K of thin diamond-like carbon (DLC) films coated with minute amount of Ni metallic particles. DLC films are deposited at room temperature by pulsed laser deposition (PLD) on a transparent quartz substrate, and Ni is deposited on the surface of DLC using molecular beam epitaxy technique at room temperature. The ultra-high vacuum thermal (range 573-873K with 60 min annealing treatments) and kinetic (range 30-3760 min at 773K) behaviors of the deposited films are investigated. Surface and interface characterizations indicate that the growth of graphitic sp2 clusters starts at temperatures lower than 573K. The kinetics of graphitization is recorded at 773K. Thus, the continuous growth of graphitic clusters leads to a long-range kinetics. These clusters are responsible for the increase in the electrical conductivity and carrier mobility, reaching values of 6.103 Siemens/cm and 20 V/cm2?s, respectively. This continuous change is not only explained by the nucleation and growth of graphitic clusters, but also by some reorientation of them alongside both the surface and the quartz substrate. The obtained results demonstrate that thermally post-treated catalytic metal/DLC films are promising materials for conductive electrodes and sensing applications.
关键词： Raman spectroscopy,thermal treatment,electric transport measurements,optical transmission and absorption,diamond-like carbon,thin graphite films,pulsed laser deposition,metal catalyst
更新于2025-09-23 15:21:01
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Sufficient condition for a quantum state to be genuinely quantum non-Gaussian
摘要： We show that the expectation value of the operator position and momentum operators x? and p? with a positive parameter c can serve as a tool to identify quantum non-Gaussian states, that is states that cannot be represented as a mixture of Gaussian states. Our condition can be readily tested employing a highly efficient homodyne detection which unlike quantum-state tomography requires the measurements of only two orthogonal quadratures. We demonstrate that our method is even able to detect quantum non-Gaussian states with positive–definite Wigner functions. This situation cannot be addressed in terms of the negativity of the phase-space distribution. Moreover, we demonstrate that our condition can characterize quantum non-Gaussianity for the class of superposition states consisting of a vacuum and integer multiples of four photons under more than 50 % signal attenuation.
关键词： Wigner functions,quantum information,continuous variables,non-Gaussian states,quadrature measurements,Gaussian state
更新于2025-09-23 15:21:01
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A Novel Topology for Process Variation-Tolerant Piezoelectric Micromachined Ultrasonic Transducers
摘要： This paper presents a novel architecture for piezoelectric micromachined ultrasonic transducers (PMUT) allowing for a drastic reduction in the impact of process variations on the accuracy of the resonant frequency. At the core of this new topology is a toroidal anchoring technique. Measurement results show that inter- and intra-die resonant frequency standard variations can be reduced from 101 kHz to 23 kHz and from 20 kHz to 5.9 kHz, respectively, by using the method proposed in this paper, showcasing devices fabricated using the PiezoMUMPs commercial fabrication technology. From the 16 chips that have been fabricated, each holding 12 PMUT devices of two different topologies, resonant frequency results are presented and analyzed to assess the effectiveness of the proposed approach. In addition, as a proof of concept, the fabricated PMUT are demonstrated to perform distance ranging measurements.
关键词： frequency,ranging measurements,variability,process variation,MEMS,PMUT,Piezoelectric ultrasonic transducers,microfabrication
更新于2025-09-23 15:21:01
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[IEEE IGARSS 2018 - 2018 IEEE International Geoscience and Remote Sensing Symposium - Valencia, Spain (2018.7.22-2018.7.27)] IGARSS 2018 - 2018 IEEE International Geoscience and Remote Sensing Symposium - Sun Induced Fluorescence Calibration and Validation for Field Phenotyping
摘要： Reliable measurements of Sun Induced Fluorescence (SIF) require a good instrument characterization as well as a complex processing chain. In this paper, we summarize the state of the art SIF retrieval methods and measurements platforms for field phenotyping. Furthermore, we use HyScreen, hyperspectral-imaging system for top of canopy measurements of SIF, as an example of the instrument requirements, data process, and data validation needed to obtain reliable measurements of SIF.
关键词： Sun Induced fluorescence,hyperspectral measurements,retrievals method,field spectrometers,field phenotyping
更新于2025-09-23 15:21:01