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Microwave-Assisted Synthesis of Quasi-Pyramidal CuInS <sub/>2</sub> -ZnS Nanocrystals for Enhanced Near-Infrared Targeted Fluorescent Imaging of Subcutaneous Melanoma
摘要： Near-infrared (NIR) fluorescent CuInS2–ZnS nanocrystals (CIZS NCs) are synthesized via an ultra-fast, non-injection microwave (MW)-assisted nanoalloying process at 230 oC within 5 min using 1-dodecanethiol (DDT) as both the sulfur source and solvent under solvothermal (ST) condition. The structural and surface analyses reveal that DDT-functionalized CIZS NCs exhibit quasi-pyramids of tetragonal-phase with well-defined facets. The DDT-functionalized CIZS NCs present a photoluminescence quantum yield (PLQY) of 76% and a long-lived fluorescence lifetime of ≈0.6 μs in organic-phase. Subsequently, DDT-functionalized CIZS NCs are phase-transferred via ligand-exchange using 11-mercaptoundecanoic acid (MUA) into water-soluble MUA–CIZS NCs that exhibit a substantial PLQY of 55%. In addition, the NIR-fluorescent MUA-functionalized CIZS NCs in conjugation with folic acid (FA), as a tumor-targeting ligand, demonstrates enhanced tumor-targeted imaging ability. The FA–MUA–CIZS NC conjugates exhibit a cell viability of ≈75% even at the highest concentration of 1 mg mL–1 and a labeling efficiency of 95.4%. The in vivo imaging results corroborate that FA–MUA–CIZS NCs conjugates are actively targeted to folate receptor-positive B16F10 tumor-bearing C57BL/6 mice in 2 h. The histopathological and hematological studies confirm no significant changes in tissue architecture and blood biochemical parameters. The confocal microscopy studies reveal deep penetration and uniform distribution of FA–MUA–CIZS NCs conjugates in subcutaneous melanoma.
关键词： CuInS2–ZnS nanocrystals,nano-bioprobe,nanoalloying approach,targeted bioimaging,microwave-solvothermal method
更新于2025-11-21 11:08:12
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Functionalized fluorescent carbon nanoparticles for sensitively targeted of folate-receptor-positive cancer cells
摘要： The folic acid-functionalized fluorescent carbon dots (FA-CDs) was synthesized via the assembly of FA to the surface of CDs. A facile hydrothermal method with proline and ethylenediamine as precursors was used to fabricate CDs. The as-prepared CDs possessed active amino groups where the CDs could be further engineered for the conjugation with FA. The uptake of the as-synthesized FA-CDs by FR positive MCF-7 cells (FR++) and HepG-2 cells (FR+) via receptor-mediated endocytosis was demonstrated by confocal laser scanning microscopy, which is further verified by a comparative study with FR-negative PC-12 cells (FR-). The bright fluorescence can be observed in FR positive MCF-7 cells while negligible fluorescence was observed in PC-12 cells with low-expressed FR, demonstrating that FA-CDs could accurately identify FR-positive cancer cells from normal cells. The FA-CDs shared favorable biocompatibility, excellent optical properties and ultra-low toxicity etc. Holding these superior properties, the FA-CDs was implemented as a highly effective platform for biological labeling and imaging, which may provide a innovative vision for cancer diagnosis and succeeding personalized therapy.
关键词： Folic acid-functionalized,Targeted bioimaging,Cancer cells,Carbon dots
更新于2025-09-23 15:22:29