- 标题
- 摘要
- 关键词
- 实验方案
- 产品
过滤筛选
- 2018
- electromagnetic pulse
- cell proliferation
- cell membrane permeability
- cell response to electromagnetic stress
- apoptosis
- cancer therapy
- necrosis
- Intelligent Medical Engineering
- V.N. Karazin Kharkiv National University
-
Improvement of Cancer Therapy by TAT Peptide Conjugated Gold Nanoparticles
摘要： Gold nanoparticles (AuNPs) are potent anticancer agent that controls drug delivery to tumors. Here, we describe the identification of TAT-Cell Penetrating Peptide (TAT-CPP) conjugated AuNPs, as a novel delivery system to the cancerous regions. TAT-peptide was modified to BSA-AuNPs [Bovine Serum Albumin (BSA) coated AuNPs] electrostatically. The binding efficiency of TAT-AuNPs was tested using Dynamic Light Scattering, UV–Vis spectrophotometer and Zeta potential. The nano-complex (BSA-AuNPs; with and without TAT-CPP) was applied against Rhabdomyosarcoma and Murine fibroblast (L20B) cancer lines, in vitro. Cytotoxicity effect was evaluated by MTT assay at 0.125, 0.25, 0.5 and 1 mg/ml concentration for 24 and 48 h incubation time. Results demonstrated that TAT-(BSA-AuNPs) exhibits significant toxicity for both cancer cell lines. TAT-CPP has improved cancer cell reduction, where cytotoxicity more than 80% has been achieved. This study was conducted to achieve the simplicity and facility in cancer therapy, where the small-sized TAT-AuNPs acts as a simple therapeutic agent in the specific delivery and targeting the deep, irregular, and complicated cancer regions in the human body. Therefore, it could replace other cancer treatment techniques, even dispense the laser irradiation in the phototherml therapy.
关键词： BSA,CPP,Small sized gold nanoparticles,Cancer treatment,TAT
更新于2025-09-23 15:23:52
-
In Situ Monitoring of Thermal Degradation of CH <sub/>3</sub> NH <sub/>3</sub> PbI <sub/>3</sub> Films by Spectroscopic Ellipsometry
摘要： High mobility group A2 (HMGA2) is an architectural transcription factor that promotes human colorectal cancer (CRC) aggressiveness by modulating the transcription of target genes. The degradation of p53 is mediated by murine double minute 2 (MDM2) in a proteasome-dependent manner. Here we report that HMGA2 promotes cell cycle progression and inhibits apoptosis in CRC cells in vitro. We also developed an intestinal epithelial cell-specific Hmga2 knock-in (KI) mouse model. It revealed that the Hmga2 KI promoted chemical carcinogen-induced tumorigenesis in the intestine in vivo. In studying the underlying molecular mechanism, we found that HMGA2 formed a protein complex with p53. The tetramerization domain of p53 (amino acids 294–393) and the three AT-hook domains (amino acids 1–83) of HMGA2 were responsible for their direct interaction. We also found that HMGA2 directly bound to MDM2 and the central acidic and zinc finger domains of MDM2 (amino acids 111–360) were required for interaction with HMGA2. Furthermore, our results indicated that HMGA2 promoted MDM2-mediated p53 ubiquitination and degradation. Interestingly, Hmga2 overexpression in Hmga2 KI mice resulted in an increase in the accumulation of ubiquitinated p53. In addition, in two large CRC cohorts, it was demonstrated that high HMGA2 expression was predictive of an adverse outcome in the p53-negative subgroup of CRC patients. In summary, our data have established for the first time a novel mechanism by which HMGA2 functions with p53 and MDM2 to promote CRC progression.
关键词： high mobility group AT-hook 2,colorectal cancer,p53
更新于2025-09-23 15:23:52
-
Development of an Aptamer-Conjugated Polyrotaxane-Based Biodegradable Magnetic Resonance Contrast Agent for Tumor-Targeted Imaging
摘要： Gadolinium-based magnetic resonance imaging (MRI) contrast agents with biodegradability, biosafety, and high efficiency are highly desirable for tumor diagnosis. Herein, a biodegradable, AS1411-conjugated, α-cyclodextrin polyrotaxane-based MRI contrast agent (AS1411-G2(DTPA-Gd)-SS-PR) was developed for targeted imaging of cancer. The polyrotaxane-based contrast agent was achieved by the complexation of α-cyclodextrin (α-CD) and a linear poly(ethylene glycol) (PEG) chain containing disulfide linkages at two terminals. The disulfides enable the de-threading of the polyrotaxane into excretable small units due to cleavage of the disulfide linkages by reducing agents such as intracellular glutathione (GSH). Furthermore, the second-generation lysine dendron conjugated with gadolinium chelates and AS1411, a G-quadruplex oligonucleotide that has high binding affinity to nucleolin generally presenting a high level on the surface of tumor cells, coupled to the α-CD via click chemistry. The longitudinal relaxivity of AS1411-G2(DTPA-Gd)-SS-PR (11.7 mM?1 s?1) was two times higher than the clinically used Gd-DTPA (4.16 mM?1 s?1) at 0.5 T. The in vitro degradability was confirmed by incubating with 10 mM 1,4-Dithiothreitol (DTT). Additionally, the cytotoxicity, histological assessment and gadolinium retention studies showed that the prepared polyrotaxane-based contrast agent had a superior biocompatibility and was predominantly cleared renally without long-term accumulation toxicity. Importantly, AS1411-G2(DTPA-Gd)-SS-PR displayed the enhanced performance in MRI of breast cancer cells in vitro as well as a subcutaneous breast tumor in vivo due to the targeting ability of AS1411 aptamer. The enhanced performance was due to efficient multivalent interactions with tumor cells, producing faster accumulation and longer contrast imaging time at the tumor site. This work clearly confirms that the specially designed and fabricated α-CD-based polyrotaxane is a promising contrast agent with excellent contrast imaging performance and biosafety for tumor MR imaging.
关键词： AS1411 aptamer,biodegradability,polyrotaxanes,magnetic resonance imaging,breast cancer targeting
更新于2025-09-23 15:23:52
-
Intratumorally Injected Photothermal Agent-Loaded Photodynamic Nanocarriers for Ablation of Orthotopic Melanoma and Breast Cancer
摘要： Traditional chemotherapy of cancers may lead to serious adverse reactions due to little drug distribution in tumors. Here, a combination of photothermal therapy (PTT) and photodynamic therapy (PDT) was used for local treatment of orthotopic melanoma and breast cancer via intratumoral (i.t.) injection of photothermal agent-loaded photodynamic nanocarriers. A hydrophobic derivative of indocyanine green, DCC, was synthesized and entrapped into a pH-sensitive photosensitizer-core copolymer, PDCZP, to form DCC@PDCZP. The nanocarriers showed remarkable fluorescence, high singlet oxygen quantum yields, and strong photothermal effect. Flow cytometry suggested that the nanocarriers were efficiently internalized by cancer cells. Near infrared thermal imaging and fluorescence self-imaging showed that the i.t. injected DCC@PDCZP mainly remained in the tumors but the intravenous (i.v.) nanocarriers were distributed a little. One i.t. injection of DCC@PDCZP was enough to ablate the orthotopic B16-F10 and 4T1 mouse tumors under 830 nm and 660 nm irradiation at 4 hours post-injection. More importantly, no local recurrences were found though scabs were formed at 9 days post-treatment. The major anticancer mechanisms included improvement of cancer cell necrosis due to hyperthermia, inhibition of neovascularization, and enhancement of cell apoptosis. The i.t. injection of PTT/PDT nanoformulations is thus a promising local treatment of superficial tumors.
关键词： zinc phthalocyanine,intratumoral injection,melanoma,indocyanine green,breast cancer,photodynamic therapy,photothermal therapy
更新于2025-09-23 15:23:52
-
Porphyrin–High-Density Lipoprotein: A Novel Photosensitizing Nanoparticle for Lung Cancer Therapy
摘要： Background. We have developed ultrasmall porphyrin–high-density lipoprotein (HDL) nanoparticles (<20 nm), called “porphyrinHDL,” that have a high density of porphyrin molecules and dissociate rapidly upon tumor cell accumulation to become ?uorescent and photoactive. This is introduced as a novel activatable photosensitizer for image-guided photodynamic therapy (PDT). Here, we report the studies of these nanoparticles targeted to scavenger receptor class B type I (SR-BI) expressed on lung cancer cells as a ?rst step toward development of a minimally invasive treatment for peripheral lung cancer and metastatic lymph nodes of advanced lung cancer. Methods. The in vitro uptake of porphyrinHDL and the corresponding PDT ef?cacy were evaluated in both SR-BI–positive and SR-BI–negative lung cancer cell lines. A clinically relevant orthotopic lung cancer model in mice was used to examine ?uorescence activation and quanti?cation of uptake in tumor. In addition, we investigated the effect of porphyrinHDL-mediated PDT. Results. PorphyrinHDL promoted proper intracellular uptake in the H460 human lung cancer cell line. When irradiated with a 671-nm PDT laser, porphyrinHDL produced signi?cant therapeutic effectiveness in vitro. After systemic administration in mice with orthotopic lung cancer xenografts, porphyrinHDL demonstrated selective accumulation and photoactivation in tumor with signi?cantly enhanced disease-to-normal tissue contrast. Moreover, porphyrinHDL-PDT signi?cantly induced cell apoptosis in lung tumors (73.2%) without toxicity in normal tissues or damage to adjacent critical structures. Conclusions. SR-BI–targeted porphyrinHDL-mediated PDT of lung cancer is selective and effective in vitro and in vivo. These initial proof-of-principle studies suggest the potential of a “smart” PDT approach for highly selective tumor ablation.
关键词： Nanoparticles,PorphyrinHDL,Lung cancer,Scavenger receptor class B type I,Photodynamic therapy
更新于2025-09-23 15:23:52
-
Ultrasensitive detection of miRNA with an antimonene-based surface plasmon resonance sensor
摘要： MicroRNA exhibits differential expression levels in cancer and can affect cellular transformation, carcinogenesis and metastasis. Although fluorescence techniques using dye molecule labels have been studied, label-free molecular-level quantification of miRNA is extremely challenging. We developed a surface plasmon resonance sensor based on two-dimensional nanomaterial of antimonene for the specific label-free detection of clinically relevant biomarkers such as miRNA-21 and miRNA-155. First-principles energetic calculations reveal that antimonene has substantially stronger interaction with ssDNA than the graphene that has been previously used in DNA molecule sensing, due to thanking for more delocalized 5s/5p orbitals in antimonene. The detection limit can reach 10 aM, which is 2.3–10,000 times higher than those of existing miRNA sensors. The combination of not-attempted-before exotic sensing material and SPR architecture represents an approach to unlocking the ultrasensitive detection of miRNA and DNA and provides a promising avenue for the early diagnosis, staging, and monitoring of cancer.
关键词： surface plasmon resonance,biosensor,antimonene,cancer diagnosis,miRNA detection
更新于2025-09-23 15:23:52
-
Therapeutic application of light emitting diode: Photo-oncomic approach
摘要： As a new light source, light emitting diode (LED) with high brightness and lower cost has been rapidly developed in medical application and light therapy. LED phototherapy can activate target cells with appropriate power and adequate energy density. This review provides general information on therapeutic applications of blue, green, yellow, red, and infrared LED in medical treatments for various physical abnormalities and on bio-imaging. The bio-imaging system is improved by decreasing the number of microscopes apparatuses including neutral-density filter, excitation filters and mechanical shutters. The numbers of excitation photons are increased and the fluorescent excitation efficiency is improved at cellular level. In the target tissue, the therapeutic effect of LEDs is dependent on incident photons irrespective of the system used to generate these photons. Photomodulated light from LED device is delivered in pulsed mode with specific pulse sequences and time. Too low or too high dose of energy may be ineffective at all. Clinical applications of LED light depending on different wavelengths are summarized. The author’s photo-oncomic experiments using a specific blue light emitting diode were introduced, showing that blue LED possessed anti-proliferative and anti-metastatic abilities in cancer cells and mice. As a promising light source, photo-oncomic approach of blue LED could be applied to treat cancers and inflammatory diseases.
关键词： cancer cell migration,photo-oncomic approach,Light emitting diode,growth
更新于2025-09-23 15:23:52
-
[IEEE 2018 IEEE Biomedical Circuits and Systems Conference (BioCAS) - Cleveland, OH, USA (2018.10.17-2018.10.19)] 2018 IEEE Biomedical Circuits and Systems Conference (BioCAS) - Development of a Portable Intraoral Camera and a Smartphone Application for Oral Cancer PDT Treatment Guidance and Monitoring
摘要： Oral cancer presents a public health crisis, particularly in regions where widespread use of tobacco, betel quid and acacia extract lead to disproportionately high incidences of oral malignancies. Photodynamic therapy (PDT) has shown to work efficiently in early-stage oral cancer lesions; however, physicians require a method capable of assisting them during therapy delivery, so treatments can be more effective. Several studies explore how to take advantage of photosensitized fluorescent lesions to perform imaging with the purpose of guiding and monitoring patients undergoing PDT; however, these streamlined systems for intraoral PDT treatment guidance and monitoring are needed in order for this approach to achieve optimal outcomes. This paper proposes the development of a smartphone application capable of processing images taken with an endoscopy camera directly connected to the device. PDT guidance and monitoring can be simplified, and treatment could be delivered more efficiently, increasing the chance of achieving a complete tumor response.
关键词： intraoral camera,smartphone application,image processing,android development,photodynamic therapy,Oral cancer,fluorescence
更新于2025-09-23 15:23:52
-
Nonrigid reconstruction of 3D breast surfaces with a low-cost RGBD camera for surgical planning and aesthetic evaluation
摘要： Accounting for 26% of all new cancer cases worldwide, breast cancer remains the most common form of cancer in women. Although early breast cancer has a favourable long-term prognosis, roughly a third of patients suffer from a suboptimal aesthetic outcome despite breast conserving cancer treatment. Clinical-quality 3D modelling of the breast surface therefore assumes an increasingly important role in advancing treatment planning, prediction and evaluation of breast cosmesis. Yet, existing 3D torso scanners are expensive and either infrastructure-heavy or subject to motion artefacts. In this paper we employ a single consumer-grade RGBD camera with an ICP-based registration approach to jointly align all points from a sequence of depth images non-rigidly. Subtle body deformation due to postural sway and respiration is successfully mitigated leading to a higher geometric accuracy through regularised locally affine transformations. We present results from 6 clinical cases where our method compares well with the gold standard and outperforms a previous approach. We show that our method produces better reconstructions qualitatively by visual assessment and quantitatively by consistently obtaining lower landmark error scores and yielding more accurate breast volume estimates.
关键词： Breast cancer treatment,Nonrigid registration,Depth camera,3D surface reconstruction,Aesthetic evaluation
更新于2025-09-23 15:23:52
-
Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation
摘要： DNA-fluorescence in situ hybridisation (DNA-FISH) allows visualisation of chromosome organisation and fluorescently labelled DNA fragments that are often produced from rearrangement. FISH probes are pools of short template plasmids that contain large genomic inserts. For effective sample penetration and target hybridisation it is critical that probe fragments are between 200 and 500bp. Production of these short probes requires significant optimisation and can be confounded access to expensive sonication equipment or inherent sequence features that influence enzymatic fragmentation or amplification. Here we demonstrate that effective FISH probes can be prepared without the need for optimisation of fragmentation using a cocktail of two the 4bp recognition sequence restriction enzymes CviQI and AluI.
关键词： Cancer,Fluorescence in situ hybridization,Translocation,FISH,Probe
更新于2025-09-23 15:23:52