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Hierarchical Core-Shell Structure of 2D VS2@VC@N-Doped Carbon Sheets Decorated by Ultrafine Pd Nanoparticles: Assembled in 3D Rosette-like Array on Carbon Fiber Microelectrode for Electrochemical Sensing
摘要： The development of two-dimension (2D) nanohybrid materials with heterogeneous component in nanoscale and three-dimension (3D) well-ordered assembly in microscale has been regarded as an effective way to improve their overall performances by synergistic coupling of the optimized structure and composition. In this work, we reported the design and synthesis of a new type of hierarchically core-shell structure of 2D VS2@VC@N-doped carbon (NC) sheets decorated by ultrafine Pd nanoparticles (PdNPs), which were vertically grown on carbon fiber (CF) and assembled into a unique 3D rosette-like array. The resultant VS2@VC@NC-PdNPs modified CF microelectrode integrated the structural and electrochemical properties of the heterogeneous hybridization of core-shell VS2@VC@NC-PdNPs sheets with unique rosette-like array structure, and gave rise to a significant improvement in terms of electron transfer ability, electrocatalytic activity, stability and biocompatibility. Under the optimized conditions, the VS2@VC@NC-PdNPs modified CF microelectrode demonstrated excellent electrochemical sensing performance towards biomarker hydrogen peroxide (H2O2) including high sensitivity of 152.7 μA cm-2 mM-1, low detection limit of 50 nM (a signal-to-noise ratio of 3:1), as well as good reproducibility and anti-interference ability, which could be used for real-time in situ electrochemical detection of H2O2 in live cancer cells and cancer tissue. The remarkable performances of the proposed nanohybrid microelectrode will have a profound impact on the design of diverse 2D layered materials as promising candidate for electrochemical biosensing applications.
关键词： electrochemical sensing,hierarchical core-shell structure,three-dimension rosette-like sheets array,Two-dimension layered nanomaterials,cancer biomarker detection
更新于2025-09-23 15:19:57
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Cancer-Specific Biomarker hNQO1-Activatable Fluorescent Probe for Imaging Cancer Cells In Vitro and In Vivo
摘要： Human NAD(P)H quinone oxidoreductase-1 (hNQO1) is an important cancer-related biomarker, which shows significant overexpression in malignant cells. Developing an effective method for detecting NQO1 activity with high sensitivity and selectivity in tumors holds a great potential for cancer diagnosis, treatment, and management. In the present study, we report a new dicyanoisophorone (DCP) based fluorescent probe (NQ-DCP) capable of monitoring hNQO1 activity in vitro and in vivo in both ratiometric and turn-on model. NQ-DCP was prepared by conjugating dicyanoisophorone fluoroprobe with hNQO1 activatable quinone propionic acid (QPA), which remain non-fluorescent until activation by tumor-specific hNQO1. NQ-DCP featured a large Stokes shift (145 nm), excellent biocompatibility, cell permeability, and selectivity towards hNQO1 allowed to differentiate cancer cells from healthy cells. We have successfully employed NQ-DCP to monitor non-invasive endogenous hNQO1 activity in brain tumor cells in vitro and in xenografted tumors developed in nude mice.
关键词： NAD(P)H quinone oxidoreductase 1,cancer biomarker,tumor diagnosis,fluorescent probe,cancer imaging,large Stokes shift,dicyanoisophorone
更新于2025-09-19 17:15:36
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Label-free quantum dot conjugates for human protein IL-2 based on molecularly imprinted polymers
摘要： Herein, the development of a fluorescent-based sensor by combining quantum dots (QDs) with molecularly-imprinted technology (MIP), intensively optimized to generate exceptional operating features is presented. This sensor is designed to target human interleukin-2 (IL-2) in synthetic human serum. IL-2 is a regulatory protein released as a triggered response from the immune system towards an inflammation. For this purpose, cadmium telluride (CdTe) QDs are prepared with 3-mercaptopropionic acid (MPA) and modified afterwards to produce an IL-2 imprinted polymer with methacrylic acid and N,N′-methylenebis(acrylamide), upon removal of the template under optimized conditions. During IL-2 rebinding, the fluorescence intensity of CdTe@MPA QDs is quenched in a concentration dependent manner. Using surface imprinting technology, the optimal fluorescence signals yielded a linear response versus logarithm of IL-2 concentration from 35 fg/ml to 39 pg/ml, in a 1000-fold diluted synthetic human serum. The limit of detection obtained is 5.91 fg/ml, lying below the concentration levels of IL-2 with clinical interest for cancer diagnosis (9.4-19.2 pg/ml). Overall, the method presented herein is a demonstration that the combination of MIP and QDs for protein detection constitutes a powerful tool in clinical analysis, providing low cost, sensitive and quick responses. The same concept may be further extended to other proteins of interest.
关键词： interleukin-2,conjugated-QDs,protein,molecularly imprinted polymer,Quantum dots,cancer biomarker
更新于2025-09-16 10:30:52
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Electrochemiluminescence Resonance Energy Transfer between Ru(bpy) <sub/>3</sub><sup>2+</sup> and CdZnSe@ZnSe Quantum Dots for Ovarian Cancer Biomarker Detection
摘要： Herein, an enhanced electrochemiluminescence resonance energy transfer (ECL-RET) from Ru(bpy)3 2+ to the core/shell CdZnSe@ZnSe quantum dots (CdZnSe@ZnSe QDs) was first designed for ovarian cancer biomarker analysis. The TiO2 metal-organic frameworks (TiO2 MOFs) was used as promoter because of its unique semiconductor structure and high loading ability for Ru(bpy)3 2+. Additionally, Envision complex with numerous horseradish peroxidase (HRP) was employed to immobilize CdZnSe@ZnSe QDs, the acceptor of ECL-RET, which further improved the ECL emission of CdZnSe@ZnSe QDs. Concretely, reactive oxygen species (ROS), for instance, O2 ?? and OH?, were firstly yielded due to the catalytic ability of HRP to H2O2, and then the electron from O2 ?? or hole from OH? injection into the CdZnSe@ZnSe QDs, triggering an extremely strong ECL response of CdZnSe@ZnSe QDs. On the basis of all above features, a highly effective ECL-RET biosensor was elaborately established to detect the targets in the range of 1.00×10-6?1.00×102 ng/mL with low detection limit of 3.30×10-1 fg/mL (S/N=3). This work opened up a new avenue for developing high-performance ECL-RET biosensors and demonstrated the high potential of the new biomarker in clinical ovarian cancer screening.
关键词： CdZnSe@ZnSe QDs,TiO2 MOFs,Envision complex,ECL-RET,ovarian cancer biomarker
更新于2025-09-12 10:27:22
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Biosynthesized Quantum Dot for Facile and Ultrasensitive Electrochemical and Electrochemiluminescence Immunoassay
摘要： Nanomaterials are commonly utilized for amplified immunoassay of biomarkers. However, traditional nanomaterial-based immunoassay usually requires time-consuming and labor-intensive nanoparticle modification and conjugation process, which impedes their practical applications. Here, a new immunoassay method based on biosynthesized nanomaterials is developed with versatile functions for facile and ultrasensitive detection of cancer biomarker. In this method, the utilized biosynthesized quantum dots (BQDs) allow convenient antibody conjugation and electrode modification, and demonstrate excellent electrochemical and electrochemiluminescent responses. The differential pulse voltammetric, impedance spectroscopy, and electrochemiluminescent measurements with the BQD-modified electrode show detection limits at picomolar levels as well as good specificity towards human prostate-specific antigen (PSA) detection. The inherent recognization capability as well as the inherent electrochemical and electrochemiluminescence features thus enable BQDs good candidates for facile immunosensors with high sensitivity. Such a biosynthesized nanomaterial-based approach opens up the possibility of using innovative designs for nanoparticle-based assays, and developing reliable and practical methods for early disease diagnosis.
关键词： Biosynthesized quantum dots,Immunoassay,Electrochemical,Electrochemiluminescence,Cancer biomarker
更新于2025-09-11 14:15:04
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Multiplexed determination of intracellular messenger RNA by using a graphene oxide nanoprobe modified with target-recognizing fluorescent oligonucleotides
摘要： A multiplexed graphene oxide (GO) fluorescent nanoprobe is described for quantification and imaging of messenger RNAs (mRNAs) in living cells. The recognizing oligonucleotides (with sequences complementary to those of target the fluorescence of the recognizing mRNAs) were labeled with different fluorescent dyes. If adsorbed on GO, oligonucleotides is quenched. After having penetrated living cells, the oligonucleotides bind to target mRNAs and dissociate from GO. This leads to the recovery of fluorescence. Using different fluorescent dyes, various intracellular mRNAs can be simultaneously imaged and quantified by a high content analysis within a short period of time. Actin mRNA acts as the internal control. This GO-based nanoprobe allows mRNA mimics to be determined within an analytical range from 1 to 400 nM and a detection limit as low as 0.26 nM. Up to 3 intracellular mRNAs (C-myc, TK1, and actin) can be detected simultaneously in a single living cell. Hence, this nanoprobe enables specific distinction of intracellular mRNA expression levels in cancerous and normal cells. It can be potentially applied as a tool for detection of cancer progression and diagnosis.
关键词： Fluorescence resonance energy transfer (FRET),Cancer biomarker,Actin mRNA,Fluorometric detection,High content analysis,Cancer diagnosis
更新于2025-09-10 09:29:36
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SERS-Based Quantification of PSMA in Tissue Microarrays Allows Effective Stratification of Patients with Prostate Cancer
摘要： Prostate specific membrane antigen (PSMA), a type II membrane protein, is an attractive biomarker that has been validated clinically for the diagnosis of prostate cancer. In this study, we developed surface-enhanced Raman scattering (SERS) nanoprobes for PSMA detection and quantification at the single-cell level on prostate cancer cells. The cells were targeted employing SERS nanoprobes that consisted of gold nanostars functionalized with PSMA aptamer molecules. We were able to quantify picomolar concentrations of soluble PSMA protein and used the resulting calibration curve to estimate the expression of PSMA on the surface of the prostate cancer cell, LNCaP, at the single-cell level. Importantly, we employed these SERS tags to stratify prostate cancer patients by assessing PSMA expression in tissues contained in a prostate tissue microarray. The stratification results clearly correlated PSMA expression to recommended therapy groups, rendering the described method as an effective tool to aid in designing personalized therapeutic protocols. Benchmarking detection sensitivity against immunofluorescence staining and comparing stratification results obtained with the two methods allowed us to validate our novel approach against standard practices. On the basis of these results, we confirm the validity of PSMA as an effective biomarker for prostate cancer patient evaluation and propose SERS-based diagnostic techniques as integrative methods for the assessment of disease stage and the identification of effective therapeutic protocols.
关键词： aptamer,tissue microarray,surface-enhanced Raman scattering,PSMA,Prostate specific membrane antigen,SERS,nanoprobes,prostate cancer,biomarker,gold nanostars
更新于2025-09-04 15:30:14