研究目的
To develop a multiplexed graphene oxide (GO) fluorescent nanoprobe for quantification and imaging of messenger RNAs (mRNAs) in living cells, enabling specific distinction of intracellular mRNA expression levels in cancerous and normal cells.
研究成果
The GO-based nanoprobe provides a new method for analyzing the spatial and dynamic distribution and expression of specific mRNAs, enabling simultaneous relative-quantification and imaging of multiplex target mRNAs in living cells with high biostability and effective fluorescence quenching.
研究不足
The GO-based nanoprobe uses only fluorescently labeled dyes in the absence of signal amplification to detect intracellular mRNA expression, making it unsuitable for analysis and imaging of extracellular RNA biomarkers in body fluids.
1：Experimental Design and Method Selection:
The study utilized a GO-based fluorescent nanoprobe for detecting mRNAs in living cells, leveraging the fluorescence quenching and recovery mechanism upon binding to target mRNAs.
2：Sample Selection and Data Sources:
HepG2 human hepatocellular liver carcinoma cell line and LO2 human normal liver cell line were used.
3：List of Experimental Equipment and Materials:
Included GO nanomaterial, recognizing oligonucleotides labeled with fluorescent dyes, and various instruments for characterization and analysis.
4：Experimental Procedures and Operational Workflow:
Involved nanoprobe preparation, intracellular mRNA imaging, and relative quantification of intracellular mRNAs.
5：Data Analysis Methods:
Fluorescence intensities were measured and normalized to Actin mRNA levels for relative quantification.
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